Toxoplasma gondii immune mapped protein 1 is anchored to the inner leaflet of the plasma membrane and adopts a novel protein fold

文献类型： 外文期刊

第一作者： Jia, Yonggen

作者： Jia, Yonggen;Dogga, Sunil Kumar;Soldati-Favre, Dominique;Benjamin, Stefi;Xu, Yingqi;Matthews, Stephen;Liu, Qun;Liu, Jing

作者机构：

关键词： Toxoplasma gondii;Acylation;Invasion;Vaccine;NMR;Protein structure

期刊名称：BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS （ 影响因子：3.036； 五年影响因子：2.969 ）

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收录情况： SCI

摘要： The immune mapped protein 1 (IMP1) was first identified as a protective antigen in Eimeria maxima and described as vaccine candidate and invasion factor in Toxoplasma gondii. We show here that TgIMP1 localizes to the inner leaflet of plasma membrane (PM) via dual acylation. Mutations either in the N-terminal myristoylation or palmitoylation sites (G2 and C5) cause relocalization of TgIMP1 to the cytosol. The first 11 amino acids are sufficient for PM targeting and the presence of lysine (1(7) is critical. Disruption of TgIMP1 gene by double homologous recombination revealed no invasion defect or any measurable alteration in the lytic cycle of tachyzoites. Following immunization with TgIMP1 DNA vaccine, mice challenged with either wild type or IMP1-ko parasites showed no significant difference in protection. The sequence analysis identified a structured C-terminal domain that is present in a broader family of IMP1-like proteins conserved across the members of Apicomplexa. We present the solution structure of this domain determined from NMR data and describe a new protein fold not seen before. (C) 2016 Elsevier B.V. All rights reserved.

分类号： Q5

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