Nuclear export signal of PRRSV NSP1 alpha is necessary for type I IFN inhibition

文献类型: 外文期刊

第一作者: Chen, Zhi

作者: Chen, Zhi;Liu, Shaoning;Sun, Wenbo;Chen, Lei;Ren, Sufang;Guo, Lihui;Cong, Xiaoyan;Li, Jun;Wu, Jiaqiang;Du, Yijun;Wang, Jinbao;Liu, Shaoning;Yoo, Dongwan;Li, Feng;Zhou, Shun

作者机构:

关键词: PRRSV;NSP1 alpha;Type I IFN;Nuclear export signal (NES)

期刊名称:VIROLOGY ( 影响因子:3.616; 五年影响因子:3.967 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: The nonstructural protein 1 alpha (NSP1 alpha) of porcine reproductive and respiratory syndrome virus (PRRSV) is a nucleo-cytoplasmic protein that suppresses the production of type I interferon (IFN). In this study, we investigated the relationship between the subcellular distribution of NSP1 alpha and its inhibition of type I IFN. NSP1 alpha was found to contain the classical nuclear export signal (NES) and NSP1 alpha nuclear export was CRM-1-mediated. NSP1 alpha was shuttling between the nucleus and cytoplasm. We also showed that the nuclear export of NSP1 alpha was necessary for its ability for type I IFN inhibition. NSP1 alpha was also found to interact with CBP, which implies a possible mechanism of CBP degradation by NSP1 alpha. Taken together, our results describe a novel mechanism of PRRSV NSP1 alpha for type I IFN inhibition and suppression of the host innate antiviral response. (C) 2016 Published by Elsevier Inc.

分类号: R37

  • 相关文献

[1]Small interfering RNA targeting nonstructural protein1 alpha (nsp1 alpha) of porcine reproductive and respiratory syndrome virus (PRRSV) can reduce the replication of PRRSV in MARC-145 cells. Shi, Xibao,Zhang, Xiaozhuan,Li, Chunxi,Shi, Xibao,Chang, Yongzhe,Zhang, Gaiping,Shi, Xibao,Chang, Yongzhe,Wang, Li,Chen, Jing,Wang, Chao,Deng, Ruiguang,Zhang, Gaiping,Zhang, Xiaozhuan,Jiang, Kai,Fan, Jianming.

[2]Molecular cloning, subcelluar location and expression profile of signal transducer and activator of transcription 2 (STAT2) from turbot, Scophthalmus maximus. Wang, Na,Chen, Song-Lin,Wang, Xian-Li,Yang, Chang-Geng.

[3]Involvement of interferon regulatory factor 3 from the barbel chub Squaliobarbus curriculus in the immune response against grass carp reovirus. Wang, Ronghua,Li, Yaoguo,Zhou, Zhiyu,Liu, Qiaolin,Zeng, Lingbing,Xiao, Tiaoyi,Wang, Ronghua,Li, Yaoguo,Liu, Qiaolin,Xiao, Tiaoyi,Zeng, Lingbing. 2018

[4]The function of feline stimulator of interferon gene (STING) is evolutionarily conserved. Zhang, Xiaozhan,Wu, Hongxia,Liu, Chunguo,Sun, Xue,Zu, Shaopo,Tian, Jin,Qu, Liandong,Li, Shoujun,Sun, Xue,Zu, Shaopo,Li, Shoujun.

[5]Interferon-omega: Current status in clinical applications. Li, Shi-fang,Zhao, Fu-rong,Shao, Jun-jun,Xie, Yin-li,Chang, Hui-yun,Zhang, Yong-guang.

[6]Identification of Type I IFN in Chinese giant salamander (Andrias davidianus) and the response to an iridovirus infection. Chen, Qian,Chen, Qian,Ma, Jie,Fan, Yuding,Meng, Yan,Xu, Jin,Zhou, Yong,Liu, Wenzhi,Zeng, Xianhui,Zeng, Lingbing.

[7]Localization, Expression Change in PRRSV Infection and Association Analysis of the Porcine TAP1 Gene. Sun, Nunu,Liu, Dewu,Meng, Fanming,Zhang, Xianwei,Wu, Zhenfang,Sun, Nunu,Liu, Xiangdong,Xie, Shengsong,Li, Xinyun,Sun, Nunu,Liu, Xiangdong,Xie, Shengsong,Li, Xinyun,Chen, Hongbo,Chen, Huiyong. 2012

[8]Porcine reproductive and respiratory syndrome virus as a vector: Immunogenicity of green fluorescent protein and porcine circovirus type 2 capsid expressed from dedicated subgenomic RNAs. Du, Yijun,Song, Cheng,Yoo, Dongwan,Pei, Yanlong,Hodgins, Douglas C.,Wu, Jiaqiang,Song, Cheng,Welch, Siao-Kun W.,Calvert, Jay G.,Li, Gang. 2009

[9]Identification of immunodominant T-cell epitopes in membrane protein of highly pathogenic porcine reproductive and respiratory syndrome virus. Tong, Guang-zhi. 2011

[10]Antigenic structure analysis of glycosylated protein 3 of porcine reproductive and respiratory syndrome virus. Zhou, YJ,An, TQ,He, YX,Liu, JX,Qiu, HJ,Wang, YF,Tong, G. 2006

[11]Generation of an infectious clone of HuN4-F112, an attenuated live vaccine strain of porcine reproductive and respiratory syndrome virus. Zhang, Shanrui,Zhou, Yanjun,Jiang, Yifeng,Li, Guoxin,Yan, Liping,Yu, Hai,Tong, Guangzhi. 2011

[12]Interference of porcine reproductive and respiratory syndrome virus replication on MARC-145 cells using DNA-based short interfering RNAs. He, Yun-xia,Hua, Rong-hong,Zhou, Yan-jun,Qiu, Hua-ji,Tong, Guang-zhi. 2007

[13]Mapping codon usage of the translation initiation region in porcine reproductive and respiratory syndrome virus genome. Su, Jun-hong,Li, Ji-dong,Ma, Xu-sheng,Dou, Yong-xi,Luo, Xue-nong,Cai, Xue-peng,Ma, Xiao-xia,He, Ya-li,Li, Ji-dong. 2011

[14]Enhanced immunogenicity induced by an alphavirus replicon-based pseudotyped baculovirus vaccine against porcine reproductive and respiratory syndrome virus. Wu, Qunfeng,Xu, Fengqin,Fang, Liurong,Xu, Jinfang,Li, Bin,Jiang, Yunbo,Chen, Huanchun,Xiao, Shaobo,Li, Bin. 2013

[15]The integrity of PRRSV nucleocapsid protein is necessary for up-regulation of optimal interleukin-10 through NF-kappa B and p38 MAPK pathways in porcine alveolar macrophages. Liu, Yanyan,Zhang, Yuyu,Zhu, Xiwang,Ren, Sufang,Guo, Lihui,Sun, Wenbo,Chen, Zhi,Cong, Xiaoyan,Chen, Lei,Du, Yijun,Li, Jun,Wu, Jiaqiang,Wang, Jinbao,Yu, Jiang,Liu, Yanyan,Zhang, Yuyu,Zhu, Xiwang,Ren, Sufang,Guo, Lihui,Sun, Wenbo,Chen, Zhi,Cong, Xiaoyan,Chen, Lei,Shi, Jianli,Du, Yijun,Li, Jun,Wu, Jiaqiang,Wang, Jinbao,Liu, Yanyan,Zhu, Xiwang,Wu, Jiaqiang,Wang, Jinbao,Liu, Xing. 2017

[16]Stable expression of foreign gene in nonessential region of nonstructural protein 2 (nsp2) of porcine reproductive and respiratory syndrome virus: Applications for marker vaccine design. Xu, Yan-Zhao,Zhou, Yan-Jun,Zhang, Shan-Rui,Jiang, Yi-Feng,Tong, Wu,Yu, Hai,Tong, Guang-Zhi.

[17]Influence of N-linked glycosylation of minor proteins of porcine reproductive and respiratory syndrome virus on infectious virus recovery and receptor interaction. Wei, Zuzhang,Tian, Debin,Sun, Lichang,Lin, Tao,Gao, Fei,Liu, Runxia,Tong, Guangzhi,Yuan, Shishan.

[18]Hemagglutinating virus of Japan envelope (HVJ-E) can enhance the immune responses of swine immunized with killed PRRSV vaccine. Dai, Zhihong,Zhang, Zhongqiu,Zhao, Deming,Dai, Zhihong,Wang, Zaishi,Zhang, Quan,Zhang, Zhongqiu,Guo, Pengju. 2011

[19]Production of porcine TNF alpha by ADAM17-mediated cleavage negatively regulates porcine reproductive and respiratory syndrome virus infection. Li, Ren,Guo, Longjun,Gu, Weihong,Luo, Xiaolei,Zhang, Jian,Xu, Yunfei,Tian, Zhijun,Feng, Li,Wang, Yue.

[20]Cloning and Sequence Analysis of ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus of the ZJ Strain. Hao, Baocheng,Niu, Dengyuan,Xing, Xiaoyong,Xiang, Haitao,Wen, Fengqin,Fu, Xiaoping,Liang, Jianping,Hu, Yonghao,Niu, Dengyuan,Liang, Jianping. 2014

作者其他论文 更多>>