The upregulation of pro-inflammatory cytokines in the rabbit uterus under the lipopolysaccaride-induced reversible immunoresponse state

文献类型： 外文期刊

第一作者： Liu, S. J.

作者： Liu, S. J.;Shi, Y.;Liu, C.;Zhang, M.;Zeng, C. J.;Zhou, G. B.;Zuo, Z. C.;Xian, H.;Song, T. Z.

作者机构：

关键词： Pro-inflammatory cytokines;Uterus;Rabbit;Lipopolysaccharide (LPS)

期刊名称：ANIMAL REPRODUCTION SCIENCE （ 影响因子：2.145； 五年影响因子：2.281 ）

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收录情况： SCI

摘要： The reproductive organs are more likely to develop gram-negative bacterial infection than other internal organs because of direct access to the body surface. The objective of this study was (1) to provide a suitable intravenous injection dose of lipopolysaccharides (LPS) instead of gram-negative bacterial infection in order to induce a reversible immunoresponse state and (2) to examine the expression levels of pro-inflammatory cytokines in the uterus of rabbits while in an immunoresponse state. Two series of experiments were performed to accomplish these objectives. In the first series, 20 healthy New Zealand White female rabbits were divided into 5 homogeneous groups (n=4), and intravenously injected with 0, 0.5, 1, 2, or 4 mg/kg body weight (BW) of LPS derived from Escherichia coli dissolved in 2 ml of sterile saline (LPS carrier). The control group received only saline. The concentrations of IL-1 beta, IL-6, and TNF-alpha in serum and the white blood cell count changed with time after LPS stimulation, and certain doses of LPS led to the death of some rabbits. The results suggested that a dose of 0.5 mg/kg of LPS induced a reversible immunoresponse state. In the second series, 4 rabbits were not injected (0 h), 16 rabbits were injected with 0.5 mg/kg LPS, and 16 rabbits in the control group were injected with 2 ml of sterile saline. Tissues of the uterine horn, uterine body, and cervix from the 36 rabbits were collected at 0, 1.5, 3, 6, and 12h (n=4) postinjection for examination of the expression levels of IL-1 beta, IL-6, and TNF-alpha by quantitative real-time PCR (qRT-PCR). The results suggested that 0.5 mg/kg of LPS upregulated the expression levels of IL-1 beta, IL-6 and TNF-alpha. in the uterine body and uterine horn, and IL-6 in the cervix. In conclusion, the expression levels of IL-1 beta, IL-6 and TNFwere upregulated in the uterus of rabbits under the reversible immunoresponse state induced by 0.5 mg/kg of LPS-injection. (C) 2016 Published by Elsevier B.V.

分类号： Q95

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