Label-free Imaging and Characterization of Cancer Cell Responses to Polymethoxyflavones Using Raman Microscopy

文献类型： 外文期刊

第一作者： Zhang, Hua

作者： Zhang, Hua;Xiao, Hang;He, Lili;Zheng, Jinkai;Liu, Anna

作者机构：

关键词： Raman microscopy;cell imaging;polymethoxyflavones;colon cancer cells;flow cytometry

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

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收录情况： SCI

摘要： We determined the cellular responses of human colon cancer HT29 and HCT116 cells to the treatments of nobiletin (NBT) and 5-demethylnobiletin (SDN) using Raman microscopy. Evaluation at both single cell and cell population levels revealed found that NBT induced more changes in the peak intensity of nucleic acid than SDN, whereas SDN induced more changes in the peak intensity of localized lipid than NBT. This result indicates the different modes of inhibitory action of these two PMFs against colon cancer cells. Between the two colon cancer cells tested, HCT116 cells were more sensitive to both PMFs than HT29 cells. The Raman data were generally in a good agreement with the flow cytometry data. Our results demonstrate that Raman microscopy is able to provide macromolecular information on cellular responses to anticancer treatments.

分类号： R15`S

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