Aptamer based photometric assay for the antibiotic sulfadimethoxine based on the inhibition and reactivation of the peroxidase-like activity of gold nanoparticles

文献类型： 外文期刊

第一作者： Yan, Jiao

作者： Yan, Jiao;Huang, Yafei;Zhang, Chenghui;Fang, Zongzhuang;Yan, Jiao;Huang, Yafei;Bai, Wenhui;Yan, Mengmeng;Zhu, Chao;Chen, Ailiang;Yan, Jiao;Huang, Yafei;Bai, Wenhui;Yan, Mengmeng;Zhu, Chao;Chen, Ailiang

作者机构：

关键词： Colorimetry;Enzyme mimetic;Catalysis;Food safety;Aptasensor;Biosensor;H2O2;Tetramethylbenzidine;Food analysis;Rapid screening

期刊名称：MICROCHIMICA ACTA （ 影响因子：5.833； 五年影响因子：5.357 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： It is known that gold nanoparticles (AuNPs) possess peroxidase-like activity. They can catalyze the oxidation of 3,3,5,5-tetramethylbenzidine by H2O2 which leads to a color change from red to blue. It is shown here that the peroxidase-like activity of AuNPs can be inhibited by passivating its surface passivation with a ssDNA aptamer against sulfadimethoxine. If, however, the target molecule (sulfadimethoxine) is present, the aptamer is desorbed from the AuNPs surface, and this results in the reactivation of the catalytic property of the AuNPs. The color change of the solution (from purple to blue) is related to the analyte concentration, and this can be judged visually or by UV-visible absorptiometry at 650 nm. The assay, under optimized conditions, has a detection limit of 10 ng center dot mL(-1) of sulfadimethoxine, and the calibration plot is linear over a rather wide concentration range (0.01-1000 mu g center dot mL(-1)). The assay can be performed within < 15 min, is sensitive, and therefore is well suited for fast screening in food analysis. Conceivably, it can be extended to many other small analytes for which aptamers are available.

分类号： O65`O655

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