A novel fluorometric turn-on assay for lipase activity based on an aggregation-induced emission (AIE) luminogen

文献类型： 外文期刊

第一作者： Shi, Jie

作者： Shi, Jie;Zhang, Shan;Zheng, Mingming;Deng, Qianchun;Zheng, Chang;Huang, Fenghong;Li, Jing

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关键词： Aggregation-induced emission;Lipase activity assay;Tetraphenylethene;Hydrolysis reaction;Fluorescence "turn-on"

期刊名称：SENSORS AND ACTUATORS B-CHEMICAL （ 影响因子：7.46； 五年影响因子：6.743 ）

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收录情况： SCI

摘要： Lipase plays critical roles in the industrial catalysis, drug discovery and medical diagnosis of diseases. In this paper, based on the consideration that a tetraphenylethylene (TPE) derivative P1 with a-COOC6H13 group can be hydrolyzed into TPE-COOH which shows low solubility in same solutions and thus aggregation can occur easily, a novel fluorescence turn-on probe bearing aggregation-induced emission (AIE) characteristics to rapidly visualize lipase activities has been constructed with compound P1. Lipase of concentrations as low as 0.1 mg mL(-1) can be detected and the assay range of lipase was located in 0.1-1.3 mg mL(-1). On the other hand, the selectivity of probe P1, dynamic monitoring of enzymatic reaction and preliminary commercially enzyme activity screening was also summarized. (C) 2016 Elsevier B.V. All rights reserved.

分类号： TP212.2

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