In vitro propagation of the endangered tree fern Cibotium barometz through formation of green globular bodies

文献类型： 外文期刊

第一作者： Yu, Rongpei

作者： Yu, Rongpei;Li, Han;Cao, Hua;Mo, Xijun;Gui, Min;Zhou, Xuhong;Jiang, Yalian;Li, Shenchong;Wang, Jihua;Yu, Rongpei;Li, Han;Cao, Hua;Mo, Xijun;Gui, Min;Zhou, Xuhong;Jiang, Yalian;Li, Shenchong;Wang, Jihua;Zhang, Guangfei

作者机构：

关键词： Cibotium barometz;In vitro propagation;Green globular bodies;Thidiazuron

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.711； 五年影响因子：2.73 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Cibotium barometz is an endangered tree fern, used both as ornamental plant and traditional Chinese medicinal plant. In this study, an effective in vitro propagation protocol was obtained through formation of green globular bodies (GGBs) from in vitro juvenile sporophytes. The effect of plant growth regulators (PGRs) on GGB induction and multiplication, as well as mineral salt concentration and active charcoal (AC) on plantlet regeneration from GGBs was evaluated. Thidiazuron (TDZ; 1-phenyl-3-(1,2,3-thiadiazol-5-yl) urea) had a significant effect on GGB induction and multiplication (P < 0.001), while a-naphthaleneacetic acid (NAA) did not (P > 0.05). GGB induction rate was above 80 % on 1/2 Murashige and Skoog (MS) media supplemented with TDZ (1.0 mg L- 1) and NAA (0.1, 0.3 or 0.5 mg L- 1). The same media were also optimal for GGB multiplication. GGBs cultured on 1/4 MS media supplemented with 0.1 or 0.2 % (w/v) AC showed a high rate of GGB development into plantlets above 90 %. 1/2 MS media supplemented with 0.1 or 0.2 % AC were the most effective for plantlet growth. Regenerated plantlets were successfully acclimatized (80 %) in greenhouse conditions. Morphological and histological analysis revealed that C. barometz GGBs was a yellow-green globular structure composed of the single GGB with meristems and hair-like structures, and new single GGBs were initiated from the epidermal cells of meristem zone.

分类号： Q942

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