gga-miR-2127 downregulates the translation of chicken p53 and attenuates chp53-mediated innate immune response against IBDV infection

文献类型： 外文期刊

第一作者： Ouyang, Wei

作者： Ouyang, Wei;Wang, Yong-shan;Meng, Kai;Pan, Qun-xing;Wang, Xiao-li;Xia, Xing-xia;Zhu, Yu-mei;Bi, Zhen-wei;Zhang, Hai-bin;Ouyang, Wei;Wang, Yong-shan;Meng, Kai;Pan, Qun-xing;Wang, Xiao-li;Xia, Xing-xia;Zhu, Yu-mei;Bi, Zhen-wei;Ouyang, Wei;Luo, Kai;Zhang, Hai-bin

作者机构：

关键词： Infectious bursal disease;chp53;miRNA;gga-miR-2127;Antiviral innate immunity

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Infectious bursal disease (IBD) is characterized by the immune suppression of infected birds. The molecular mechanism by which IBD virus (IBDV) suppresses the host immune system remains to be elucidated. The tumor suppressor protein p53 can inhibit the replication of various viruses, but its effect on IBDV remains unknown. This study established an in vitro infection model based on DF-1 cells (chicken embryo fibroblast cell line) to investigate the antiviral effects of chicken p53 (chp53) on IBDV infection. The expression level and activity of chp53 remarkably increased in IBDV-infected DF-1 cells. The overexpression of chp53 inhibited IBDV replication and upregulated the expression of multiple chicken antiviral innate immunity genes (IPS-1, IRF3, PKR, OAS, and Mx), whereas the suppression of chp53 led to the opposite effect. This result indicates that chp53 activates the antiviral innate immune response of chickens to IBDV infection. Bioinformatics analysis and dual-luciferase reporter assay showed that gga-miR-2127 targeted the 3'UTR of chp53. qRT-PCR and western blot revealed that gga-miR-2127 overexpression in DF-1 cells not only downregulated the expression levels of chp53 and of the antiviral innate immunity genes in chickens but also promoted IBDV replication. Our results suggest that gga-miR-2127 downregulates chp53 mRNA translation by targeting its 3'UTR and attenuates chp53-mediated antiviral innate immune response against IBDV. (C) 2016 Elsevier B.V. All rights reserved.

分类号： S8

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