Overexpression of OsMYB1R1-VP64 fusion protein increases grain yield in rice by delaying flowering time
文献类型: 外文期刊
第一作者: Wang, Jiachang
作者: Wang, Jiachang;Xu, Yang;Zhang, Huan;Cai, Maohong;Wang, Chunming;Wan, Jianmin;Wu, Fuqing;Zhu, Shanshan;Cheng, Zhijun;Wang, Jiulin;Li, Chaonan;Sheng, Peike;Guo, Xiuping;Zhang, Xin;Wan, Jianmin
作者机构:
关键词: flowering time;grain yield;VP64
期刊名称:FEBS LETTERS ( 影响因子:4.124; 五年影响因子:3.814 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: VP64 is widely used as a transcriptional activator to investigate the biological function of genes, but its potential for application in genetic improvement of crops has not been fully investigated. Here, we characterized an OsMYB1R1-VP64 fusion protein that enhanced the grain yield of rice cultivar Kita-ake' by 35%. OsMYB1R1-VP64 regulated grain yield of transgenic plants mainly by extending the vegetative growth stage. Further analysis indicated that OsMYB1R1-VP64 delayed flowering by down-regulating expression of Heading date 3a (Hd3a) and RICE FLOWERING LOCUST 1 (RFT1) through repression of Early heading date 1 (Ehd1). The expression pattern of OsMYB1R1 was constitutive and rhythmically controlled. OsMYB1R1 protein had transcriptional activation activity and was localized to the nucleus. Our findings suggest that the OsMYB1R1-VP64 construct can be used to increase grain yield in rice.
分类号: Q5
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