Evaluating an indirect rMPSP enzyme-linked immunosorbent assay for the detection of bovine Theileria infection in China

文献类型： 外文期刊

第一作者： Zhao, Shuaiyang

作者： Zhao, Shuaiyang;Liu, Junlong;Zhao, Hongxi;Li, Youquan;Xie, Junren;Liu, Aihong;Hassan, Muhammad-Adeel;Yin, Hong;Guan, Guiquan;Luo, Jianxun;Zhao, Hongxi;Yin, Hong

作者机构：

关键词： Bovine theileriosis;MPSP;ELISA;Theileria sinensis;Prevalence

期刊名称：PARASITOLOGY RESEARCH （ 影响因子：2.289； 五年影响因子：2.403 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Bovine theileriosis, a tick-borne protozoan disease caused by Theileria annulata, Theileria orientalis and Theileria sinensis, is widespread in China and is a serious economic problem for the Chinese livestock industry. In this study, recombinant major piroplasma surface proteins (MPSP) of T. annulata, T. orientalis and T. sinensis based on MPSP genes were expressed in Escherichia coli BL21(DE3). The immunogenicity and specificity of the three purified recombinant MPSP proteins were evaluated with the reference positive sera of T. annulata, T. orientalis, T. sinensis, Babesia bovis, B abesia bigemina, Babesia major, Babesia motasi, Theileria luwenshuni, Theileria uilenbergi and Anaplasma ovis using an enzyme-linked immunosorbent assay (ELISA) or western blotting. The results showed that all three of the rMPSP proteins had a strong reaction with the sera from cattle infected with T. annulata, T. orientalis and T. sinensis via western blotting but not with other piroplasma and Anaplasma species. Then, the rMPSP protein of T. sinensis was used to develop an iELISA for detecting the three Theileria species infections. The specificity and sensitivity were 95.7 and 95.5 %, respectively, with a threshold of 28.8 % of the specific mean antibody rate (AbR). Finally, 2473 field-collected bovine sera, from 42 prefectures of 17 provinces in China, were tested using the ELISA to evaluate the prevalence of bovine theileriosis, and the average positive rate was 43.6 %. The developed iELISA could be a suitable tool to detect the three bovine Theileria species, and the data also provided important information regarding the current prevalence of bovine theileriosis in China.

分类号： R53

  • 相关文献

[1]Seroprevalence of bovine theileriosis in northern China. Li, Yaqiong,Liu, Zhijie,Liu, Junlong,Yang, Jifei,Li, Qian,Guo, Pengfei,Guan, Guiquan,Liu, Guangyuan,Luo, Jianxun,Yin, Hong,Li, Youquan,Yin, Hong. 2016

[2]Merozoite proteins from Babesia sp BQ1 (Lintan) as potential antigens for serodiagnosis by ELISA. Guan, G. Q.,Chauvin, A.,Moreau, E.,Guan, G. Q.,Luo, J. X.,Yin, H.,Rogniaux, H.. 2010

[3]Detecting and differentiating Theileria sergenti and Theileria sinensis in cattle and yaks by PCR based on major piroplasm surface protein (MPSP). Liu, Aihong,Guan, Guiquan,Liu, Zhijie,Liu, Junlong,Li, Youquan,Gao, Jinliang,Ma, Milin,Niu, Qinli,Ren, Qiaoyun,Bai, Qi,Yin, Hong,Luo, Jianxun,Leblanc, Neil. 2010

[4]Additional data for a new Theileria sp from China based on the sequences of ribosomal RNA internal transcribed spacers. Liu, Junlong,Guan, Guiquan,Liu, Zhijie,Liu, Aihong,Ma, Miling,Bai, Qi,Yin, Hong,Luo, Jianxun. 2013

[5]A novel zoonotic Anaplasma species is prevalent in small ruminants: potential public health implications. Yang, Jifei,Liu, Zhijie,Niu, Qingli,Liu, Junlong,Han, Rong,Guan, Guiquan,Hassan, Muhammad Adeel,Liu, Guangyuan,Luo, Jianxun,Yin, Hong,Yin, Hong. 2017

[6]Porcine circovirus type 2 in China: an update on and insights to its prevalence and control. Zhai, Shao-Lun,Sun, Bei-Bei,Deng, Su-Fang,Hu, Jun,Luo, Man-Lin,Zhai, Shao-Lun,Xu, Zhi-Hong,Lv, Dian-Hong,Wen, Xiao-Hui,Yuan, Jie,Wei, Wen-Kang,Chen, Sheng-Nan,Tang, Man-Hua,Wang, Feng-Guo,Li, Xiao-Jing. 2014

[7]Stray dogs as indicators of Toxoplasma gondii distributed in the environment: the first report across an urban-rural gradient in China. Yan, Chao,Yue, Cai-Ling,Wang, Dong-Hui,Zhou, Dong-Hui,Yan, Chao,Fu, Lin-Lin,Yue, Cai-Ling,Tang, Ren-Xian,Liu, Yi-Sheng,Zhang, Peng,Wang, Dong-Hui,Zheng, Kui-Yang,Lv, Liang,Shi, Na,Zeng, Ping,Zhu, Xing-Quan,Zhu, Xing-Quan. 2012

[8]Toxoplasma gondii Infection in Immunocompromised Patients: A Systematic Review and Meta-Analysis. Wang, Ze-Dong,Wei, Feng,Wang, Ze-Dong,Zhu, Xing-Quan,Wang, Ze-Dong,Liu, Huan-Huan,Ma, Hong-Yu,Li, Zhong-Yu,Liu, Quan,Ma, Zhan-Xi,Yang, Zhi-Bin,Xu, Bin,Liu, Quan. 2017

[9]Prevalence of porcine circovirus-like agent P1 in Jiangsu, China. Wen, Libin,He, Kongwang,Yu, Zhengyu,Mao, Aihua,Zhong, Shulin,Ni, Yanxiu,Zhang, Xuehan,Li, Bin,Wang, Xiaomin,Zhou, Junming,Guo, Rongli,Lv, Lixin,Jiang, Jieyuan,Yang, Hanchun. 2011

[10]Prevalence of Hydatid Cysts in Livestock Animals in Xinjiang, China. Meng Qingling,Qiao Jun,Liu Tianli,Song Xuemei,Chen Chuangfu,Zhu Xinquan,Wang Guanglei,Zhang Jinsheng,Wang Huisheng,Cai Kuojun.

[11]Prevalence, intensity and associated factor analysis of Tropilaelaps mercedesae infesting Apis mellifera in China. Luo, Qi-Hua,Zhou, Ting,Dai, Ping-Li,Song, Huai-Lei,Wu, Yan-Yan,Wang, Qiang,Luo, Qi-Hua,Zhou, Ting,Dai, Ping-Li,Song, Huai-Lei,Wu, Yan-Yan,Wang, Qiang.

[12]Prevalence and genetic characterization of Toxoplasma gondii infection in bats in southern China. Jiang, H. H.,Qin, S. Y.,Liu, Q.,Zhu, X. Q.,Wang, W.,He, B.,Tu, C. C.,Liu, Q.,Hu, T. S.,Fan, Q. S.,Wu, J. M.,Qin, S. Y.,Zhu, X. Q..

[13]Prevalence and molecular characterization of Cryptosporidium spp. in dairy cattle, northwest China. Zhang, Xiao-Xuan,Tan, Qi-Dong,Zhou, Dong-Hui,Ni, Xiao-Ting,Liu, Guang-Xue,Yang, Yan-Chuan,Zhu, Xing-Quan,Zhang, Xiao-Xuan,Tan, Qi-Dong.

[14]Molecular characterization of Giardia duodenalis from white yaks in China. Song, Guang-Yao,Song, Ming-Xin,Qin, Si-Yuan,Zhu, Xing-Quan,Zhou, Dong-Hui,Zhao, Guang-Hui,Zhu, Xing-Quan.

[15]Prevalence, risk factors and molecular characterization of Enterocytozoon bieneusi in raccoon dogs (Nyctereutes procyonoides) in five provinces of Northern China. Xu, Chao,Zhang, Xiao-Xuan,Rui-Du,Wang, Quan-Kai,Zhao, Quan,Xu, Chao,Zhang, Hao,Zhao, Jia-Ping,Ba, Heng-Xing,Xing, Xiu Mei,Ma, Xusheng. 2016

[16]Occurrence and multilocus genotyping of Giardia intestinalis assemblage C and D in farmed raccoon dogs, Nyctereutes procyonoides, in China. Zhang, Xiao-Xuan,Zheng, Wen-Bin,Ma, Jian-Gang,Yao, Qiu-Xia,Zou, Yang,Bubu, Cai-Jia,Zhu, Xing-Quan,Zhang, Xiao-Xuan,Zheng, Wen-Bin,Ma, Jian-Gang,Zou, Yang,Bubu, Cai-Jia,Zhao, Quan,Zhu, Xing-Quan,Yao, Qiu-Xia,Zhu, Xing-Quan. 2016

[17]Detection prevalence of H5N1 avian influenza virus among stray cats in eastern China. Zhao, Fu-Rong,Zhou, Dong-Hui,Zhang, Yong-Guang,Shao, Jun-Jun,Lin, Tong,Li, Yang-Fan,Wei, Ping,Chang, Hui-Yun,Zhao, Fu-Rong,Zhou, Dong-Hui,Zhang, Yong-Guang,Shao, Jun-Jun,Lin, Tong,Li, Yang-Fan,Wei, Ping,Chang, Hui-Yun,Wei, Ping.

[18]Prevalence, risk factors and multilocus genotyping of Enterocytozoon bieneusi in farmed foxes (Vulpes lagopus), Northern China. Zhang, Xiao-Xuan,Cong, Wei,Lou, Zhi-Long,Ma, Jian-Gang,Zheng, Wen-Bin,Yao, Qiu-Xia,Zhu, Xing-Quan,Zhang, Xiao-Xuan,Cong, Wei,Lou, Zhi-Long,Ma, Jian-Gang,Zheng, Wen-Bin,Zhao, Quan,Zhu, Xing-Quan,Yao, Qiu-Xia,Zhu, Xing-Quan. 2016

[19]Evaluation of different nested PCRs for detection of Anaplasma phagocytophilum in ruminants and ticks. Yang, Jifei,Liu, Zhijie,Niu, Qingli,Liu, Junlong,Xie, Jingying,Chen, Qiuyu,Chen, Ze,Guan, Guiquan,Liu, Guangyuan,Luo, Jianxun,Yin, Hong,Yin, Hong. 2016

[20]Prevalence of Toxoplasma gondii in Dogs in Zhanjiang, Southern China. Jiang, Hai-Hai,Li, Ming-Wei,Xu, Min-Jun,Cong, Wei,Zhu, Xing-Quan,Li, Ming-Wei,Cong, Wei,Zhu, Xing-Quan. 2015

作者其他论文 更多>>

微信小程序