A protease-resistant alpha-galactosidase from Pleurotus djamor with broad pH stability and good hydrolytic activity toward raffinose family oligosaccharides

文献类型: 外文期刊

第一作者: Hu, Yujing

作者: Hu, Yujing;Wang, Hexiang;Hu, Yujing;Wang, Hexiang;Tian, Guoting;Zhao, Liyan;Tzi Bun Ng

作者机构:

关键词: alpha-Galactosidase;Pleurotus djamor;Protease-resistant;Raffinose family oligosaccharides

期刊名称:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES ( 影响因子:6.953; 五年影响因子:6.737 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: An acidic alpha-galactosidase designated as PDGI (Pleurotus djamor alpha-galactosidase) was purified to homogeneity with 290-fold purification and a specific activity of 52.18 units/mg by means of ion exchange chromatography and gel filtration chromatography. PDGI is a monomeric protein exhibiting a molecular mass of 60 kDa in SDS-PAGE and gel filtration. The optimum pH and temperature of the enzyme with pNPGal as substrate were 5.0 and 53.5 degrees C, respectively. It displayed great pH stability within the pH range 3.0-10.0. Besides, the enzyme harbored remarkable resistance to acid protease and varying degrees of tolerance to other proteases: trypsin > collagenase Type-I> alpha-chymotrypsin neutral protease > proteinase K. It was strongly inhibited by K-+,K- Cd2+, Cu2+, Hg2+, Al3+, Fe3+ and Ag+ ions. The chemical modification reagents diethypyrocarbonate (DEPC), 2,3-butanedione (DIC) and trinitrophenol (TNBS) increased the activity of PDGI 1.5-fold whereas N-bromosuccinimide (NBS) and parachloro-mercuri-benzoate (PCMB) drastically suppressed its activity. PDGI displayed activity toward stachyose and raffinose. The Km values for hydrolysis of pNPGal, stachyose and raffinose were 0.76, 7.63 and 6.29 mM, respectively. Furthermore, PDGI degraded raffinose and sthachyose. These results suggest that PDGI has great potential for elimination of the non-digestible and flatulence-causing oligosaccharides stachyose and raffinose from legumes. (C) 2016 Elsevier B.V. All rights reserved.

分类号: Q71

  • 相关文献

[1]A protease-resistant alpha-galactosidase from Pleurotus citrinopileatus with broad substrate specificity and good hydrolytic activity on raffinose family oligosaccharides. Hu, Yujing,Geng, Xueran,Zhang, Weiwei,Wang, Hexiang,Hu, Yujing,Geng, Xueran,Zhang, Weiwei,Wang, Hexiang,Tian, Guoting,Zhao, Liyan,Ng, Tzi Bun. 2016

[2]Isolation of a protease-resistant and pH-stable alpha-galactosidase displaying hydrolytic efficacy toward raffinose family oligosaccharides from the button mushroom Agaricus bisporus. Hu, Yujing,Hu, Yujing,Wang, Hexiang,Hu, Yujing,Wang, Hexiang,Zhu, Mengjuan,Tian, Guoting,Zhao, Liyan,Ng, Tzi Bun.

[3]Molecular Cloning and Expression of a Novel Protease-resistant GH-36 alpha-Galactosidase from Rhizopus sp F78 ACCC 30795. Yanan Cao,Wang, Yaru,Luo, Huiying,Shi, Pengjun,Meng, Kun,Zhou, Zhigang,Yao, Bin,Zhang, Zhifang. 2009

[4]Regulation of Seed Vigor by Manipulation of Raffinose Family Oligosaccharides in Maize and Arabidopsis thaliana. Li, Tao,Zhang, Yumin,Wang, Dong,Liu, Ying,Zhao, Tianyong,Li, Tao,Zhang, Yumin,Wang, Dong,Liu, Ying,Zhao, Tianyong,Dirk, Lynnette M. A.,Downie, A. Bruce,Goodman, Jack,Wang, Jianmin,Wang, Guoying. 2017

[5]Preparation and characterization of alpha-galactosidase-loaded chitosan nanoparticles for use in foods. Liu, Yong,Li, Yanli,Xu, Shaochun,Tang, Jiangwu,Xu, Yaoxing,Sun, Yan,Ding, Juntao. 2011

[6]Molecular cloning and characterization of a novel alpha-galactosidase gene from Penicillium sp F63 CGMCC 1669 and expression in Pichia pastoris. Mi, Shijun,Meng, Kun,Wang, Yaru,Bai, Yingguo,Yuan, Tiezheng,Luo, Huiying,Yao, Bin. 2007

[7]Gene cloning, expression and characterization of an alpha-galactosidase from Pedobacter nyackensis MJ11 CGMCC 2503 with potential as an aquatic feed additive. Liu, Xiaodan,Meng, Kun,Wang, Yaru,Shi, Pengjun,Yuan, Tiezheng,Yang, Peilong,Luo, Huiying,Bai, Yingguo,Yao, Bin,Liu, Xiaodan. 2009

[8]Biochemical characterization of a novel thermophilic alpha-galactosidase from Talaromyces leycettanus JCM12802 with significant transglycosylation activity. Wang, Caihong,Wang, Huimin,Ma, Rui,Shi, Pengjun,Niu, Canfang,Luo, Huiying,Yang, Peilong,Yao, Bin,Ma, Rui,Yang, Peilong.

[9]A Fungal alpha-Galactosidase from Tricholoma matsutake with Broad Substrate Specificity and Good Hydrolytic Activity on Raffinose Family Oligosaccharides. Geng, Xueran,Wang, Hexiang,Tian, Guoting,Zhao, Yongchang,Zhao, Liyan,Ng, Tzi Bun.

[10]Insights into the substrate specificity and synergy with mannanase of family 27 alpha-galactosidases from Neosartorya fischeri P1. Wang, Huimin,Ma, Rui,Shi, Pengjun,Huang, Huoqing,Yang, Peilong,Wang, Yaru,Yao, Bin,Ma, Rui,Fan, Yunliu,Yang, Peilong.

[11]Properties of a novel alpha-galactosidase from Streptomyces sp. S27 and its potential for soybean processing. Cao, Yanan,Yuan, Tiezheng,Shi, Pengjun,Luo, Huiying,Li, Ning,Meng, Kun,Bai, Yingguo,Yang, Peilong,Zhou, Zhigang,Yao, Bin,Zhang, Zhifang. 2010

[12]A new alpha-galactosidase from symbiotic Flavobacterium sp. TN17 reveals four residues essential for alpha-galactosidase activity of gastrointestinal bacteria. Zhou, Junpei,Shi, Pengjun,Huang, Huoqing,Cao, Yanan,Meng, Kun,Yang, Peilong,Zhang, Rui,Chen, Xiaoyan,Yao, Bin,Zhou, Junpei. 2010

[13]A novel protease-resistant alpha-galactosidase with high hydrolytic activity from Gibberella sp F75: gene cloning, expression, and enzymatic characterization. Cao, Yanan,Wang, Yaru,Meng, Kun,Bai, Yingguo,Shi, Pengjun,Luo, Huiying,Yang, Peilong,Zhou, Zhigang,Yao, Bin,Zhang, Zhifang. 2009

[14]A thermophilic alpha-galactosidase from Neosartorya fischeri P1 with high specific activity, broad substrate specificity and significant hydrolysis ability of soymilk. Wang, Huimin,Shi, Pengjun,Luo, Huiying,Huang, Huoqing,Yang, Peilong,Yao, Bin. 2014

[15]Cloning and functional expression of an alpha-galactosidase from Yersinia pestis biovar Microtus str. 91001. Cao, Yanan,Huang, Huoqing,Meng, Kun,Yang, Peilong,Shi, Pengjun,Wang, Yaru,Luo, Huiying,Yao, Bin,Zhang, Zhifang,Wu, Ningfeng. 2008

[16]A New alpha-Galactosidase from Thermoacidophilic Alicyclobacillus sp A4 with Wide Acceptor Specificity for Transglycosylation. Wang, Huimin,Ma, Rui,Shi, Pengjun,Xue, Xianli,Luo, Huiying,Huang, Huoqing,Bai, Yingguo,Yang, Peilong,Yao, Bin. 2014

[17]Purification and characterization of a novel protease-resistant alpha-galactosidase from Rhizopus sp. F78 ACCC 30795. Cao, Yanan,Yang, Peilong,Shi, Pengjun,Wang, Yaru,Luo, Huiying,Meng, Kun,Zhang, Zhifang,Wu, Ningfeng,Yao, Bin,Fan, Yunliu.

[18]A Fungal Alpha-Galactosidase from Pseudobalsamia microspora Capable of Degrading Raffinose Family Oligosaccharides. Yang, Dongxue,Du, Fang,Wang, Hexiang,Yang, Dongxue,Du, Fang,Wang, Hexiang,Tian, Guoting,Zhao, Yongchang,Zhao, Liyan,Tzi Bun Ng.

[19]Dynamic characteristics of sugar accumulation and related enzyme activities in sweet and non-sweet watermelon fruits. Liu, Jingan,Guo, Shaogui,He, Hongju,Zhang, Haiying,Gong, Guoyi,Ren, Yi,Xu, Yong,Liu, Jingan,Guo, Shaogui,He, Hongju,Zhang, Haiying,Gong, Guoyi,Ren, Yi,Xu, Yong.

作者其他论文 更多>>