Effective synthesis of theaflavin-3,3 '-digallate with epigallocatechin-3-O-gallate and epicatechin gallate as substrates by using immobilized pear polyphenol oxidase

文献类型： 外文期刊

第一作者： Lei, Shicheng

作者： Lei, Shicheng;Xie, Minhao;Hu, Bing;Zhou, Li;Sun, Yi;Saeeduddin, Muhammad;Zeng, Xiaoxiong;Lei, Shicheng;Zhang, Hongcheng

作者机构：

关键词： Polyphenol oxidase;Huangguan pear;Fe3O4/chitosan nanoparticles;Immobilization;Theaflavin-3;3 '-digallate;Synthesis

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： In the present study, pear polyphenol oxidase (PPO) was purified, immobilized and applied for the synthesis of theaflavin-3,3'-digallate (TF3). Firstly, PPO of pear (Pyrus bretschneideri Rehd cv. Huangguan) was purified 24.36-fold in specific activity with a recovery of 6.77% by sequential use of precipitation with 70% saturated ammonium sulfate and chromatography of DEAE-Sepharose Fast Flow column. The purified pear PPO, found to be a dimer of identical subunits of molecular mass 35 kDa, was then successfully immobilized onto Fe3O4/chitosan nanoparticles via glutaraldehyde coupling reaction. Finally, TF3 was effectively synthesized by applying the immobilized PPO as biocatalyst with epicatechin gallate (ECG) and epigallocatechin-3-O-gallate (EGCG) as substrates, and the maximum yield of TF3 was 42.23% based on the amount of ECG added. In addition, the immobilized enzyme still remained 85% of its initial ability after successive usage of 8 cycles and could be stored for 30 days with less loss of activity. All the results suggest that the developed procedure is quite useful and has great potential for synthesis of TF3 and other theaflavins. (C) 2016 Elsevier B.V. All rights reserved.

分类号： Q71

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