ROS accumulation and antiviral defence control by microRNA528 in rice

文献类型： 外文期刊

第一作者： Wu, Jianguo

作者： Wu, Jianguo;Yang, Zhirui;Yao, Shengze;Zhao, Shanshan;Wang, Yu;Jin, Lian;Li, Yi;Wu, Jianguo;Xie, Lianhui;Yang, Rongxin;Song, Xianwei;Chu, Chengcai;Cao, Xiaofeng;Yang, Rongxin;Song, Xianwei;Chu, Chengcai;Cao, Xiaofeng;Li, Pingchuan;Zhou, Tong;Lan, Ying;Zhou, Xueping;Qi, Yijun;Cao, Xiaofeng

作者机构：

期刊名称：NATURE PLANTS （ 影响因子：15.793； 五年影响因子：17.349 ）

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收录情况： SCI

摘要： MicroRNAs (miRNAs) are key regulators of plant-pathogen interactions. Modulating miRNA function has emerged as a new strategy to produce virus resistance traits(1-5). However, the miRNAs involved in antiviral defence and the underlying mechanisms remain largely elusive. We previously demonstrated that sequestration by Argonaute (AGO) proteins plays an important role in regulating miRNA function in antiviral defence pathways(6). Here we reveal that cleavage-defective AGO18 complexes sequester microRNA528 (miR528) upon viral infection. We show that miR528 negatively regulates viral resistance in rice by cleaving L-ascorbate oxidase (AO) messenger RNA, thereby reducing AO-mediated accumulation of reactive oxygen species. Upon viral infection, miR528 becomes preferentially associated with AGO18, leading to elevated AO activity, higher basal reactive oxygen species accumulation and enhanced antiviral defence. Our findings reveal a mechanism in which antiviral defence is boosted through suppression of an miRNA that negatively regulates viral resistance. This mechanism could be manipulated to engineer virus-resistant crop plants.

分类号： Q94

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