A sensitive duplex nanoparticle-assisted PCR assay for identifying porcine epidemic diarrhea virus and porcine transmissible gastroenteritis virus from clinical specimens

文献类型： 外文期刊

第一作者： Zhu, Yu

作者： Zhu, Yu;Wang, Chunren;Cui, Yudong;Cui, Shangjin;Zhu, Yu;Liang, Lin;Luo, Yakun;Cui, Shangjin;Wang, Guihua;Ai, Xia

作者机构：

关键词： Duplex nanoPCR;N gene;PEDV;TGEV;Clinical diagnosis

期刊名称：VIRUS GENES （ 影响因子：2.332； 五年影响因子：2.0 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： In this study, a novel duplex nanoparticle-assisted polymerase chain reaction (nanoPCR) assay was developed to detect porcine epidemic diarrhea virus (PEDV) and porcine transmissible gastroenteritis virus (TGEV). Two pairs of primers were designed based on the conserved region within the N gene of PEDV and TGEV. In a screening of 114 clinical samples from four provinces in China for PEDV and TGEV, 48.2 and 3.5 % of the samples, respectively, tested positive. Under optimized conditions, the duplex nanoPCR assay had a detection limit of 7.6 x 10(1) and 8.5 x 10(1) copies mu L-1 for PEDV and TGEV, respectively. The sensitivity of the duplex nanoPCR assay was ten times higher than that of a conventional PCR assay. Moreover, no fragments were amplified when the duplex nanoPCR assay was used to test samples containing other porcine viruses. Our results indicate that the duplex nanoPCR assay described here is useful for the rapid detection of PEDV and TGEV and can be applied in clinical diagnosis.

分类号： R37

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