Generation and evaluation of a genetically attenuated Newcastle disease virus rGM-VIIm as a genotype-matched vaccine
文献类型: 外文期刊
第一作者: Sun, Minhua
作者: Sun, Minhua;Xiang, Bin;Li, Yaling;Xie, Peng;Gao, Shimin;Kang, Yinfeng;Gao, Pei;Li, Yanling;Wang, Zhaoxiong;Liang, Jianpeng;Yu, Deshui;Ren, Tao;Sun, Minhua;Xiang, Bin;Li, Yaling;Xie, Peng;Gao, Shimin;Kang, Yinfeng;Gao, Pei;Li, Yanling;Wang, Zhaoxiong;Liang, Jianpeng;Yu, Deshui;Ren, Tao;Sun, Minhua;Xiang, Bin;Li, Yaling;Xie, Peng;Gao, Shimin;Kang, Yinfeng;Gao, Pei;Li, Yanling;Wang, Zhaoxiong;Liang, Jianpeng;Yu, Deshui;Ren, Tao;Sun, Minhua;Xiang, Bin;Li, Yaling;Xie, Peng;Gao, Shimin;Kang, Yinfeng;Gao, Pei;Li, Yanling;Wang, Zhaoxiong;Liang, Jianpeng;Yu, Deshui;Ren, Tao;Sun, Minhua;Gao, Shimin;Kang, Yinfeng;Wang, Zhaoxiong
作者机构:
关键词: Newcastle disease virus;Genotype VII;Reverse genetics;Vaccine
期刊名称:VIRUS GENES ( 影响因子:2.332; 五年影响因子:2.0 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Despite intensive vaccination campaigns, outbreaks of Newcastle disease (ND) have been frequently reported in China, especially of genotype VII that first emerged in the late 1990s. Given the dire need for vaccines against the circulating genotype VII virus, we developed an alternative method to recover a highly virulent recombinant GM (rGM) virus that involves a T7 system with a hammerhead ribozyme sequence introduced downstream of the T7 promoter. By changing the F-0 polybasic cleavage site RRQKRa dagger"F to the monobasic GRQGRa dagger"L, we generated a mutant virus (rGM-VIIm) that was found to be highly attenuated in chickens. The rGM-VIIm virus not only produced fourfold higher hemagglutination assay (HA) titers than the parental virus, but also exhibited genetic stability after 15 continuous passages in specific-pathogen-free (SPF) embryonated eggs. Whether live or inactivated, rGM-VIIm and LaSota vaccines can protect vaccinated birds from GM challenge infection. However, live and inactivated rGM-VIIm vaccines reduced virus shedding of the homologous challenge virus significantly better than the LaSota virus vaccine did. Altogether, our results suggest that rGM-VIIm vaccines could aid in the control of ND in China.
分类号: R37
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