Organ- and Growing Stage-Specific Expression of Solanesol Biosynthesis Genes in Nicotiana tabacum Reveals Their Association with Solanesol Content

文献类型： 外文期刊

第一作者： Yan, Ning

作者： Yan, Ning;Zhang, Hongbo;Zhang, Zhongfeng;Du, Yongmei;Liu, Xinmin;Liu, Yanhua;Shi, John;Timko, Michael P.

作者机构：

关键词： Nicotiana tabacum;solanesol;RNA-seq;solanesyl diphosphate synthase;gene expression;chlorophyll

期刊名称：MOLECULES （ 影响因子：4.411； 五年影响因子：4.587 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Solanesol is a noncyclic terpene alcohol that is composed of nine isoprene units and mainly accumulates in solanaceous plants, especially tobacco (Nicotiana tabacum L.). In the present study, RNA-seq analyses of tobacco leaves, stems, and roots were used to identify putative solanesol biosynthesis genes. Six 1-deoxy-D-xylulose 5-phosphate synthase (DXS), two 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR), two 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (IspD), four 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (IspE), two 2-C-methyl-D-erythritol 2,4-cyclo-diphosphate synthase (IspF), four 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase (IspG), two 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase (IspH), six isopentenyl diphosphate isomerase (IPI), and two solanesyl diphosphate synthase (SPS) candidate genes were identified in the solanesol biosynthetic pathway. Furthermore, the two N. tabacum SPS proteins (NtSPS1 and NtSPS2), which possessed two conserved aspartate-rich DDxxD domains, were highly homologous with SPS enzymes from other solanaceous plant species. In addition, the solanesol contents of three organs and of leaves from four growing stages of tobacco plants corresponded with the distribution of chlorophyll. Our findings provide a comprehensive evaluation of the correlation between the expression of different biosynthesis genes and the accumulation of solanesol, thus providing valuable insight into the regulation of solanesol biosynthesis in tobacco.

分类号： O62

  • 相关文献

[1]Exogenous auxin regulates multi-metabolic network and embryo development, controlling seed secondary dormancy and germination in Nicotiana tabacum L.. Li, Zhenhua,Wang, Jianhua,Li, Zhenhua,Zhang, Jie,Zhao, Jiehong,Ren, Xueliang,Liu, Yiling,Fu, Junjie,Wang, Guoying. 2016

[2]ECTOPIC EXPRESSION OF SUBUNIT A OF VACUOLAR H+-ATPASE FROM APPLE ENHANCES SALT TOLERANCE IN TOBACCO PLANTS. Dong, Q. L.,Liu, D. D.,Wang, Q. J.,Fang, M. J.,Hao, Y. J.,Yao, Y. X.,Dong, Q. L.,Liu, D. D..

[3]Construction of vector with IMT1 and its gene expression in transgenic tobacco leaf cells associated with salt tolerance. Dong, YZ. 1999

[4]Transcriptome Analysis of Sucrose Metabolism during Bulb Swelling and Development in Onion (Allium cepa L.). Zhang, Chunsha,Zhang, Hongwei,Liang, Yi,Zhan, Zongxiang,Liu, Bingjiang,Chen, Zhentai. 2016

[5]De novo Transcriptome Assembly of Chinese Kale and Global Expression Analysis of Genes Involved in Glucosinolate Metabolism in Multiple Tissue. Wu, Shuanghua,Lei, Jianjun,Chen, Guoju,Cao, Bihao,Chen, Changming,Chen, Hancai. 2017

[6]Gene expression changes leading extreme alkaline tolerance in Amur ide (Leuciscus waleckii) inhabiting soda lake. Xu, Jian,Li, Qiang,Xu, Liming,Jiang, Yanliang,Zhao, Zixia,Zhang, Yan,Li, Jiongtang,Dong, Chuanju,Xu, Peng,Sun, Xiaowen,Wang, Shaolin,Dong, Chuanju. 2013

[7]Global gene expression responses to waterlogging in roots of sesame (Sesamum indicum L.). Wang, Linhai,Zhang, Yanxin,Qi, Xiaoqiong,Li, Donghua,Wei, Wenliang,Zhang, Xiurong. 2012

[8]Systemic analysis of gene expression profiles in porcine granulosa cells during aging. Li Hui,Guo Shuangshuang,Yu Jianning,Shi Zhendan. 2017

[9]DE NOVO TRANSCRIPTOME ANALYSIS OF MULBERRY (MORUS L.) UNDER DROUGHT STRESS USING RNA-SEQ TECHNOLOGY. Wang, Heng,Tong, Wei,Feng, Li,Jiao, Qian,Long, Li,Fang, Rongjun,Zhao, Weiguo,Long, Li,Zhao, Weiguo,Fang, Rongjun,Zhao, Weiguo.

[10]Development of a 44 K custom oligo microarray using 454 pyrosequencing data for large-scale gene expression analysis of Camellia sinensis. Wang, Lu,Wang, Xinchao,Yue, Chuan,Cao, Hongli,Zhou, Yanhua,Yang, Yajun,Wang, Lu,Wang, Xinchao,Zhou, Yanhua,Wang, Xinchao,Yue, Chuan,Cao, Hongli,Yang, Yajun.

[11]Genome-Wide Identification and Expression Analysis of the WRKY Gene Family in Cassava. Wei, Yunxie,Shi, Haitao,Xia, Zhiqiang,Tie, Weiwei,Ding, Zehong,Yan, Yan,Wang, Wenquan,Hu, Wei,Li, Kaimian. 2016

[12]Comparative Transcriptome Analysis Reveals Differential Transcription in Heat-susceptible and Heat-tolerant Pepper (Capsicum annum L.) Cultivars under Heat Stress. Li, Tao,Xu, Xiaowan,Li, Ying,Wang, Hengming,Li, Zhiliang,Li, Zhenxing,Li, Tao,Xu, Xiaowan.

[13]RNA-seq based gene expression analysis of ovarian granulosa cells exposed to zearalenone in vitro: significance to steroidogenesis. Zhang, Guo-Liang,Zhang, Rui-Qian,Sun, Xiao-Feng,Cheng, Shun-Feng,Wang, Yu-Feng,Ge, Wei,Zhao, Yong,Shen, Wei,Li, Lan,Zhang, Guo-Liang,Ji, Chuan-Liang,Yu, Jie,Zhang, Guo-Liang,Sun, Shi-Duo,Feng, Yan-Zhong. 2017

[14]Transciptomic and histological analysis of hepatopancreas, muscle and gill tissues of oriental river prawn (Macrobrachium nipponense) in response to chronic hypoxia. Sun, Shengming,Fu, Hongtuo,Zhu, Jian,Ge, Xianping,Xuan, Fujun,Gu, Zhimin. 2015

[15]Transcriptome comparison in the pituitary-adrenal axis between Beagle and Chinese Field dogs after chronic stress exposure. Luo, Wei,Xu, Haiping,Nie, Qinghua,Luo, Wei,Xu, Haiping,Nie, Qinghua,Luo, Wei,Xu, Haiping,Nie, Qinghua,Fang, Meixia,Xing, Huijie.

[16]De novo assembly and characterization of Muscovy duck liver transcriptome and analysis of differentially regulated genes in response to heat stress. Zeng, Tao,Zhang, Liping,Li, Jinjun,Wang, Deqian,Tian, Yong,Lu, Lizhi,Zhang, Liping.

[17]Selection of endogenous reference genes for gene expression analysis in the mediterranean species of the Bemisia tabaci (Hemiptera: Aleyrodidae) complex. Su, Yun-Lin,He, Wen-Bo,Liu, Shu-Sheng,Wang, Xiao-Wei,Wang, Jia,Li, Jun-Min.

[18]Solanesol: a review of its resources, derivatives, bioactivities, medicinal applications, and biosynthesis. Yan, Ning,Liu, Yanhua,Gong, Daping,Du, Yongmei,Zhang, Huaibao,Zhang, Zhongfeng. 2015

[19]Solanesol Biosynthesis in Plants. Yan, Ning,Liu, Yanhua,Zhang, Hongbo,Du, Yongmei,Liu, Xinmin,Zhang, Zhongfeng.

[20]Virus resistance obtained in transgenic tobacco and rice by RNA interference using promoters with distinct activity. Zhang, C.,Song, Y.,Jiang, F.,Jiang, Y.,Zhu, C.,Wen, F.,Li, G..

作者其他论文 更多>>

微信小程序