Development of one-tube multiplex polymerase chain reaction (PCR) for detecting Mycobacterium bovis

文献类型: 外文期刊

第一作者: Quan, Zhang

作者: Quan, Zhang;Haiming, Tan;Xiaoya, Cai;Weifeng, Yuan;Hong, Jia;Hongfei, Zhu

作者机构:

关键词: differential diagnosis;multiplex PCR;Mycobacterium bovis;Mycobacterium tuberculosis

期刊名称:JOURNAL OF VETERINARY MEDICAL SCIENCE ( 影响因子:1.267; 五年影响因子:1.348 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: A multiplex PCR (m-PCR) with primers targeting the 16S rRNA, Rv3873 and a 12.7-kb fragment in the genomes of a Mycobacterium tuberculosis complex was designed for the differential diagnosis of M. tuberculosis, M bovis, M bovis BCG and non-tuberculosis Mycobacterium (NTM). The specificity of this assay was 100%, and the detection limit was 15 pg of genomic DNA. Of the 206 blinded clinical samples, the detection rate of M. bovis infection by m-PCR was lower than that of the interferon gamma (IFN-gamma) release assay; however, the false-positive rate by the tuberculin skin test and false-negative samples in the IFN-gamma release assay were reduced. Our findings indicated that our m-PCR method is a useful tool for complementation to differentiate M bovis from M tuberculosis and NTM species. KEY WORDS: differential diagnosis, multiplex PCR, Mycobacterium bovis, Mycobacterium tuberculosis

分类号: S85

  • 相关文献

[1]Recombinant TB9.8 of Mycobacterium bovis Triggers the Production of IL-12 p40 and IL-6 in RAW264.7 Macrophages via Activation of the p38, ERK, and NF-kappa B Signaling Pathways. Jia, Hong,Liu, Shuqing,Wu, Jing,Hou, Shaohua,Xin, Ting,Guo, Xiaoyu,Yuan, Weifeng,Zhang, Gaimei,Li, Ming,Zhu, Hongfei,Gao, Xintao,Qu, Hongfei,Zhu, Hongfei.

[2]Recombinant TB10.4 of Mycobacterium bovis induces cytokine production in RAW264.7 macrophages through activation of the MAPK and NF-kappa B pathways via TLR2. Liu, Shuqing,Jia, Hong,Hou, Shaohua,Zhang, Gaimei,Xin, Ting,Yuan, Weifeng,Guo, Xiaoyu,Li, Ming,Wu, Jing,Zhu, Hongfei,Li, Hegang,Li, Hegang,Gao, Xintao. 2014

[3]Marek's disease virus VP22 enhances potentially the immune response of ESAT-6/CFP-10 against Mycobacterium bovis infection. Chen, Hongjun,Wang, Wei,Song, Cuiping,Yu, Shengqing,Ding, Chan. 2010

[4]Rapid and simultaneous detection of Mycobacterium bovis and Mycobacterium avium subsp paratuberculosis in invasive flies by duplex PCR. Zhang, Jihong,Zheng, Shijun,Zhang, Jihong,Li, Rusong,Huang, Suwen,Wang, Jianfeng,Ni, Jianbo,Zhao, Xiuling,Huang, Shaotang,Zhu, Tangming,Liu, Siguo,Zhu, Ting,Sun, Jing. 2013

[5]A novel DNA vaccine for protective immunity against virulent Mycobacterium bovis in mice. Liu, Siguo,Gong, Qiang,Wang, Chunlai,Liu, Huifang,Wang, Yong,Guo, Sheping,Liu, Jiandong,Shao, Meili,Chi, Lei,Zhao, Kun,Kong, Xiangang,Gong, Qiang,Wang, Yong,Shao, Meili,Wang, Weili,Wang, Zhenguo,Shi, Yuanxiang,Huang, Ying,guli, Aman,Zhang, Chunsheng,Wang, Chunlai,Wang, Chunlai. 2008

[6]Development of a combined canine distemper virus specific RT-PCR protocol for the differentiation of infected and vaccinated animals (DIVA) and genetic characterization of the hemagglutinin gene of seven Chinese strains demonstrated in dogs. Yi, Li,Cheng, Shipeng,Xu, Hongli,Wang, Jianke,Cheng, Yuening,Yang, Shen,Luo, Bin. 2012

[7]A multiplex nested RT-PCR for the detection and differentiation of wild-type viruses from C-strain vaccine of classical swine fever virus. Li, Yan,Zhao, Jian-Jun,Li, Na,Shi, Zixue,Cheng, Dan,Zhu, Qing-Hu,Tu, Changchun,Tong, Guang-Zhi,Qiu, Hua-Ji. 2007

[8]A safe and molecular-tagged Brucella canis ghosts confer protection against virulent challenge in mice. Qian, Jing,Bu, Zhaoyang,Lang, Xulong,Wang, Xinglong,Qian, Jing,Qian, Jing,Yan, Guangmou,Yang, Yanling,Wang, Xiuran.

[9]Differential Diagnosis of Antibody to Classical Swine Fever Virus Field Strain by ELISA with Recombinant E2 Proteins of Various Group CSFV. Qiu Chang-Qing,Hu Hui,Cao Xiao-An,Zhou Ji-Zhang,Lin Guo-Zhen,Hu Hui. 2008

[10]Establishment of reverse transcription loop-mediated isothermal amplification for rapid detection and differentiation of canine distemper virus infected and vaccinated animals. Liu, Da-Fei,Chai, Hong-Liang,Yin, Xiu-Chen,Hua, Yu-Ping,Liu, Da-Fei,Liu, Chun-Guo,Tian, Jin,Zhang, Xiao-Zhan,Yang, Tian-Kuo,Zhang, Hong-Ying,Liu, Ming,Qu, Lian-Dong,Jiang, Yi-Tong.

[11]Evaluation of a real-time RT-PCR assay using minor groove binding probe for specific detection of Chinese wild-type classical swine fever virus. Wen, Guoyuan,Yang, Jun,Luo, Qingping,Liao, Yonghong,Zhang, Rongrong,Wang, Hongling,Ai, Diyun,Luo, Ling,Shao, Huabin,Zhang, Tao,Wen, Guoyuan,Hu, Zhibin,Song, Nianhua,Zhang, Tao. 2011

[12]Identification of a virus-specific and conserved B-cell epitope on NS1 protein of Japanese encephalitis virus. Tong, Guang-Zhi,Bin Wang,Hua, Rong-Hong,Tian, Zhi-Jun,Chen, Na-Sha,Zhao, Fu-Rong,Liu, Tian-Qiang,Wang, Yun-Feng,Tong, Guang-Zhi. 2009

[13]Effects of Mycobacterium tuberculosis ESAT6-CFP10 Protein on Cell Viability and Production of Nitric Oxide in Alveolar Macrophages. Xie, Xiaoli,Han, Meng,Zhang, Liang,Liu, Laixing,Gu, Zuye,Yang, Mei,Yang, Hongjun. 2016

[14]Crystallization and preliminary X-ray analysis of a novel esterase Rv0045c from Mycobacterium tuberculosis. Xu, Lipeng,Liu, Siguo,Xu, Lipeng,Guo, Jiubiao,Zheng, Xiangdong,Pang, Hai,Wen, Tingyi,Sun, Fei. 2010

[15]Combined recombinant DNA vaccine results in significant protection against Mycobacterium tuberculosis. Pan, Y,Cai, H,Li, SX,Tian, X,Li, T,Zhu, YX. 2003

[16]Efficient tuberculosis treatment in mice using chemotherapy and immunotherapy with the combined DNA vaccine encoding Ag85B, MPT-64 and MPT-83. Yu, D-H,Cai, H.,Hu, X-D. 2008

[17]Characterization of Rv0394c gene encoding hyaluronidase and chondrosulfatase from Mycobacterium tuberculosis. Yuan, Xianglian,Chen, Liping,Cao, Jun,Yu, Shenye,Liu, Siguo,Yuan, Xianglian,Deng, Xiaoxia,Pang, Hai,Wang Quankai. 2013

[18]Characterization of the putative tryptophan synthase beta-subunit from Mycobacterium tuberculosis. Shen, Hongbo,Yang, Yanping,Wang, Feifei,Xu, Shengfeng,Wang, Honghai,Zhang, Ying,Ye, Naihao. 2009

[19]Characterization of mutations in streptomycin-resistant Mycobacterium tuberculosis isolates in Sichuan, China and the association between Beijing-lineage and dual-mutation in gidB. Sun, Honghu,Xiang, Ling,Pi, Rui,Guo, Zhen,Zheng, Chao,Li, Song,Sun, Qun,Zhang, Congcong,Zhao, Yuding,Tang, Ke,Luo, Mei,Rastogi, Nalin,Li, Yuqing. 2016

[20]Characterization of a novel exported esterase Rv3036c from Mycobacterium tuberculosis. Chen, Liping,Dang, Guanghui,Cao, Jun,Yu, Shenye,Liu, Siguo,Dang, Guanghui,Deng, Xiaoxia,Pang, Hai,Dang, Guanghui,Wu, Defeng.

作者其他论文 更多>>