Identification and characterization of expressed TIR- and non-TIR-NBS-LRR resistance gene analogous sequences from radish (Raphanus sativus L.) de novo transcriptome

文献类型： 外文期刊

第一作者： Wang, Yan

作者： Wang, Yan;Song, Zhaohui;Zhang, Wei;Xu, Liang;Muleke, Everlyne M'mbone;Liu, Liwang;Su, Xiaojun

作者机构：

关键词： Radish (Raphanus sativus L.);Resistance gene analogues (RGAs);Nucleotide-binding site leucine-rich repeat (NBS-LRR);Transcriptome sequencing

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Radish is an important root vegetable crop worldwide. However, it is susceptible to a variety of diseases leading to decline in yield and quality. The plant nucleotide-binding site (NBS)-leucine-rich repeat (LRR) gene superfamily is effective against a big range of pathogens. Therefore, the isolation and identification of such R gene analogue (RGA) is a critical foundation for improving disease resistance in plants. So far, however, no comprehensive isolation of NBS-LRR RGAs has been reported in radish. In this study, a total of 36,613 unigenes representing 81,481 unique transcripts with an average length of 1253.22 nt were obtained from radish leaf transcriptome. In total, 38 NBS-encoding sequences were successfully identified via bioinformatics analysis. Multiple sequence alignment analysis classified these sequences into toll and interleukin-1 receptor (TIR)-NBS-LRR and non-TIR-NBS-LRR subclasses based on the presence of a TIR domain at the N-terminus. Phylogenetic tree analysis of the deduced amino acid sequences grouped these sequences into six classes. Non-synonymous to synonymous substitution ratio suggested that NBS-encoding sequences of RGAs in radish were subject to purification or negative selection. Additionally, RT-qPCR analysis revealed that several RsRGAs expressed differentially under three tested abiotic stimuli (SA, MeJA and ABA), suggesting that they might be involved in defense responses by activating signaling pathways. These results could provide valuable information for further identification of plant RGAs and accelerate genetic improvement of disease resistances in radish breeding programs. (C) 2016 Elsevier B.V. All rights reserved.

分类号： S6

  • 相关文献

[1]Identification of novel and salt-responsive miRNAs to explore miRNA-mediated regulatory network of salt stress response in radish (Raphanus sativus L.). Sun, Xiaochuan,Xu, Liang,Wang, Yan,Yu, Rugang,Luo, Xiaobo,Gong, Yiqin,Wang, Ronghua,Limera, Cecilia,Liu, Liwang,Sun, Xiaochuan,Xu, Liang,Luo, Xiaobo,Zhu, Xianwen,Zhang, Keyun. 2015

[2]A comparative light and electron microscopic analysis of microspore and tapetum development in fertile and cytoplasmic male sterile radish. Shi, Shengli,Ding, Ding,Wang, Jianbo,Mei, Shiyong.

[3]Comparative analysis of pre- and post-parasitic transcriptomes and mining pioneer effectors of Heterodera avenae. Yang, Dan,Chen, Changlong,Liu, Qian,Jian, Heng,Chen, Changlong. 2017

[4]Genome-Wide Association and Transcriptome Analyses Reveal Candidate Genes Underlying Yield-determining Traits in Brassica napus. Lu, Kun,Peng, Liu,Zhang, Chao,Lu, Junhua,Yang, Bo,Xiao, Zhongchun,Liang, Ying,Xu, Xingfu,Qu, Cunmin,Zhang, Kai,Liu, Liezhao,Li, Jiana,Peng, Liu,Zhang, Chao,Zhu, Qinlong,Fu, Minglian,Yuan, Xiaoyan. 2017

[5]High-throughput sequencing of highbush blueberry transcriptome and analysis of basic helix-loop-helix transcription factors. Song Yang,Liu Hong-di,Zhang Hong-jun,Wang Hai-bo,Liu Feng-zhi,Zhou Qiang,Zhang Zhi-dong,Li Ya-dong. 2017

[6]Comparative transcriptome analysis of soybean response to bean pyralid larvae. Sun, Zudong,Cai, Zhaoyan,Chen, Huaizhu,Lai, Zhenguang,Yang, Shouzhen,Tang, Xiangmin. 2017

[7]Comparative transcriptome analysis of Ziziphus jujuba infected by jujube witches' broom phytoplasmas. Fan, Xin-Ping,Liu, Wei,Qiao, Yong-Sheng,Shang, Yong-Jin,Wang, Guo-Ping,Tian, Xin,Fan, Xin-Ping,Liu, Wei,Shang, Yong-Jin,Wang, Guo-Ping,Han, Yuan-Huai,Bertaccini, Assunta. 2017

[8]Comparative transcriptome profiling of a rice line carrying Xa39 and its parents triggered by Xanthomonas oryzae pv. oryzae provides novel insights into the broad-spectrum hypersensitive response. Zhang, Fan,Huang, Li-Yu,Zhang, Fan,Zhuo, Da-Long,Li, Zhi-kang,Zhou, Yong-Li,Huang, Li-Yu,Ali, Jauhar,Cruz, Casiana Vera,Du, Zheng-Lin. 2015

[9]Transcriptome Analysis and Discovery of Genes Involved in Immune Pathways from Coelomocytes of Sea Cucumber (Apostichopus japonicus) after Vibrio splendidus Challenge. Gao, Qiong,Gao, Qiong,Liao, Meijie,Wang, Yingeng,Li, Bin,Zhang, Zheng,Rong, Xiaojun,Chen, Guiping,Wang, Lan. 2015

[10]Gonadal transcriptomic analysis of yellow catfish (Pelteobagrus fulvidraco): identification of sex-related genes and genetic markers. Lu, Jianguo,Luan, Peixian,Zhang, Xiaofeng,Xue, Shuqun,Peng, Lina,Sun, Xiaowen,Lu, Jianguo,Lu, Jianguo,Luan, Peixian,Zhang, Xiaofeng,Xue, Shuqun,Sun, Xiaowen,Peng, Lina,Mahbooband, Shahid.

[11]Transcriptome analysis of nitrogen-starvation-responsive genes in rice. Yang, Wenzhu,Yoon, Jinmi,Choi, Heebak,An, Gynheung,Yang, Wenzhu,Yoon, Jinmi,Choi, Heebak,An, Gynheung,Yang, Wenzhu,Fan, Yunliu,Chen, Rumei,Yang, Wenzhu,Fan, Yunliu,Chen, Rumei. 2015

[12]Embryonal Control of Yellow Seed Coat Locus ECY1 Is Related to Alanine and Phenylalanine Metabolism in the Seed Embryo of Brassica napus. Wang, Fulin,Shi, Jianghua,Zheng, Tao,Wu, Guanting,Liu, Renhu,He, Jiewang,Xu, Fei,Liu, Renhu,Liu, Shengyi. 2016

[13]ThPP1 gene, encodes an inorganic pyrophosphatase in Thellungiella halophila, enhanced the tolerance of the transgenic rice to alkali stress. He, Rui,Han, Xiaori,He, Rui,Yu, Guohong,Han, Jiao,Li, Wei,Wang, Bing,Huang, Shengcai,Cheng, Xianguo,Han, Jiao.

[14]PeanutDB: an integrated bioinformatics web portal for Arachis hypogaea transcriptomics. Shu, Changlong,Zhang, Jie,Schmidt, Emily,Li, Pei,Lenox, Douglas,Liu, Lin,Liang, Chun,Schmidt, Emily,Lenox, Douglas,Liang, Chun. 2012

[15]Gene Expression Variations of Red-White Skin Coloration in Common Carp (Cyprinus carpio). Li, Xiao-Min,Song, Ying-Nan,Xiao, Gui-Bao,Zhu, Bai-Han,Xu, Gui-Cai,Sun, Ming-Yuan,Xiao, Jun,Sun, Xiao-Wen,Li, Jiong-Tang,Li, Xiao-Min,Song, Ying-Nan,Xiao, Gui-Bao,Zhu, Bai-Han,Xu, Gui-Cai,Sun, Ming-Yuan,Xiao, Jun,Sun, Xiao-Wen,Li, Jiong-Tang,Song, Ying-Nan,Zhu, Bai-Han,Sun, Ming-Yuan,Xiao, Jun,Mahboob, Shahid,Al-Ghanim, Khalid A.. 2015

[16]Analysis on differentially expressed genes in watermelon rind color based on RNA-Seq. Wu Cai-jun,Yang Kan-kan,Liang Zhi-huai. 2016

[17]Transcriptome analysis revealed the drought-responsive genes in Tibetan hulless barley. Zeng, Xingquan,Wei, Zexiu,Yuan, Hongjun,Wang, Yulin,Xu, Qijun,Tang, Yawei,Nyima, Tashi,Yuan, Hongjun,Wang, Yulin,Xu, Qijun,Tang, Yawei,Nyima, Tashi,Bai, Lijun. 2016

[18]De novo assembly of the transcriptome of Neottopteris nidus using Illumina paired-end sequencing and development of EST-SSR markers. Jia, Xinping,Deng, Yanming,Sun, Xiaobo,Liang, Lijian,Su, Jiale.

[19]Comparative analysis of the liver transcriptome of Pelteobagrus vachellii with an alternative feeding time. Wen, Zhengyong,Zou, Yuanchao,Yuan, Dengyue,Shao, Ting,Li, Huatao,Gong, Quan.

[20]Differential gene expression in banana roots in response to Fusarium wilt. Wang, Yuguang,Xia, Qiyu,Lu, Xuehua,Sun, Jianbo,Wang, Guihua,Zhang, He,Zhang, Xin.

作者其他论文 更多>>

微信小程序