Newly identified CSP41b gene localized in chloroplasts affects leaf color in rice

文献类型： 外文期刊

第一作者： Mei, Jiasong

作者： Mei, Jiasong;Liu, Xuri;Hu, Guocheng;Fu, Yaping;Liu, Wenzhen;Li, Feifei

作者机构：

关键词： CSP4Ib;Rice;Map -based cloning;LGL1;Leaf color;Chloroplast

期刊名称：PLANT SCIENCE （ 影响因子：4.729； 五年影响因子：5.132 ）

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收录情况： SCI

摘要： A rice mutant with light -green leaves was discovered from a transgenic line of Oryza sativa. The mutant has reduced chlorophyll content and abnormal chloroplast morphology throughout its life cycle. Genetic analysis revealed that a single nuclear -encoded recessive gene is responsible for the mutation, here designated as Ig11. To isolate the lgll gene, a high -resolution physical map of the chromosomal region around the lgll gene was made using a mapping population consisting of 1984 mutant individuals. The Ig11 gene was mapped in the 76.5 kb region between marker YG4 and marker YG5 on chromosome 12. Sequence analysis revealed that there was a 39 bp deletion within the fourth exon of the candidate gene 0s12g0420200 (TIGR locus Os12g23180) encoding a chloroplast stem -loop -binding protein of 41 kDa b (CSP41b). The lgll mutation was rescued by transformation with the wild type CSP41b gene. Accordingly, the CSP41b gene is identified as the LGL1 gene. CSP41b was transcribed in various tissues and was mainly expressed in leaves. Expression of CSP41b-GFP fusion protein indicated that CSP4lb is localized in chloroplasts. The expression levels of some key genes involved in chlorophyll biosynthesis and photosynthesis, such as ChID, Chll, Hemel, Ygll, POR, CabiR, Cab2R, PsaA, and rbcL, was significantly changed in the lgll mutant. Our results demonstrate that CSP41b is a novel gene required for normal leaf color and chloroplast morphology in rice. (c) 2016 Elsevier Ireland Ltd. All rights reserved.

分类号： Q94

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