Influence of extraction and solubilizing treatments on the molecular structure and functional properties of peanut protein

文献类型： 外文期刊

第一作者： Ma, Tiezheng

作者： Ma, Tiezheng;Zhu, Hongguang;Wang, Jing;Yu, Liangli (Lucy);Sun, Baoguo;Wang, Qiang;Yu, Liangli (Lucy)

作者机构：

关键词： Solubilizing treatment;Peanut protein;Molecular structure;Functional properties

期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY （ 影响因子：4.952； 五年影响因子：5.383 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： As primary functional property of protein in food industry, solubility was enhanced by various solubilization methods. Peanut protein was used to prepare product with high solubility by physical and enzymatic hydrolysis methods. Effects of extraction and solubilizing treatments on protein structure were evaluated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR). Changes of functional properties including emulsifying, water-holding, fat-binding, foaming and viscosity properties were observed and interpreted. Modification treatments which could improve the solubility were almost suitable for the promotion of emulsifying, fat-binding and foaming capacities, but not always suitable for the improvement of viscosity and water-holding properties. SDS-PAGE showed average molecular weight remained unaffected after physical modifications combined by high-speed stirring and ultrasonic treatment while reduced after limited enzymatic hydrolysis and high-pressure homogenization. The microstructure of protein shown by SEM was gradually disbanded after all modification treatments. The regularity of protein secondary structure, which was damaged during alcohol extraction, was reconstructed by combined physical modification and limited enzymatic hydrolysis. Depending on the demand of functional properties on final product, manufacture process could be optimized by the selection of modification treatments based on observing the changes in protein structures. (C) 2017 Elsevier Ltd. All rights reserved.

分类号： TS2

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