Variable responses of ammonia oxidizers across soil particle-size fractions affect nitrification in a long-term fertilizer experiment
文献类型: 外文期刊
第一作者: Zhang, Qian
作者: Zhang, Qian;Liang, Guoqing;Zhou, Wei;Myrold, David D.
作者机构:
关键词: Particle-size fraction;Fertilization;Ammonia-oxidizers;qPCR;T-RFLP;454 Pyrosequencing
期刊名称:SOIL BIOLOGY & BIOCHEMISTRY ( 影响因子:7.609; 五年影响因子:8.312 )
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年卷期:
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收录情况: SCI
摘要: We used soils from a 33-year fertilizer experiment and performed high-throughput pyrosequencing analyses targeting the ammonia monooxygenase alpha subunit (amoA) gene (also qPCR and terminal restricted fragment length polymorphism (T-RFLP)) to determine whether and how the composition and structure of ammonia-oxidizing microbes in different particle-size fractions (>2000, 2000-200, 200-63, 63-2 and 2-0.1 mu m) have changed in response to chronic inorganic (NPK) and organic (NPKM) fertilizer additions under a rice-wheat rotation. We found fertilization and particle-size fractions had strong effects on abundance and structure of ammonia-oxidizers and potential nitrification activity (PNA) (P < 0.05) which was significantly enhanced under NPKM across all soil fractions. PNA was higher in clay fraction and lowest in silt fraction which was largely correlated to total N and C:N ratio. Aggregated boosted tree emphasized the role that total N and C:N ratio play on ammonia oxidizer abundance. Pyrosequencing data revealed that the Nitrososphaera and Nitrosospira were the dominant clusters of ammonia-oxidizing archaea (AOA) and bacteria (AOB), respectively. Even though AOA population was hundreds of times greater than AOB, it seemed AOB responded more sensitively to ambient shifts which was convinced by NMDS analyses based on Bray-Curtis distance of TRFs and also of OTUs from 454 pyrosequencing. (C) 2016 Elsevier Ltd. All rights reserved.
分类号: S1
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