Fluorescence resonance energy transfer biosensor between upconverting nanoparticles and palladium nanoparticles for ultrasensitive CEA detection

文献类型： 外文期刊

第一作者： Li, Hui

作者： Li, Hui;Li, Peiwu;Li, Hui;Li, Peiwu;Li, Hui;Li, Peiwu;Li, Hui;Li, Peiwu;Li, Hui;Li, Peiwu;Shi, Liang;Sun, De-en;Liu, Zhihong

作者机构：

关键词： Palladium nanoparticles;Fluorescence quenching ability;Upconverting nanoparticles;Carcinoembryonic antigen;Fluorescence resonance energy transfer

期刊名称：BIOSENSORS & BIOELECTRONICS （ 影响因子：10.618； 五年影响因子：9.323 ）

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页码：

收录情况： SCI

摘要： An ultrasensitive biosensor for carcinoembryonic antigen (CEA) was constructed based on fluorescence resonance energy transfer (FRET) between upconverting nanoparticles (UCPs) and palladium nano particles (PdNPs). PdNPs was synthesized by the addition of a solution of Na2PdCl4 into a mixture of N2H4 center dot H2O as the reducing agent and 11-mercaptoundecanoic acid (MUDA) as the stabilizer. The CEA aptamer (5'-NH2-ATACCAGCTFATTCAATT-3') was conjugated to hexanedioic acid (HDA) modified UCPs (HDA-UCPs) through an EDC-NHS coupling protocol. The coordination interaction between nitrogen functional groups of the CEA aptamer and PdNPs brought UCPs and PdNPs in close proximity, which resulted in the fluorescence quenching of UCPs to an extent of 85%. And the non-specific fluorescence quenching caused by PdNPs towards HDA-UCPs was negligible. After the introduction of CEA into the UCPs-CEA aptamer-PdNPs fluorescence quenching system, the CEA aptamer preferentially combined with CEA accompanied by the conformational change which weakened the coordination interaction between the CEA aptamer and PdNPs. So fluorescence recovery of UCPs was observed and a linear relationship between the fluorescence recovery of UCPs and the concentration of CEA was obtained in the range from 2 pg/mL to 100 pg/mL in the aqueous buffer with the detection limit of 0.8 pg/mL. The ultrasensitive detection of CEA was also realized in diluted human serum with a linear range from 4 pg/mL to 100 pg/mL and a detection limit of 1.7 pg/mL. This biosensor makes the most of the high quenching ability of PdNPs towards UCPs with negligible non-specific fluorescence quenching and has broad application prospects in biochemistry. (C) 2016 Elsevier B.V. All rights reserved.

分类号： Q

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