WSL3, a component of the plastid-encoded plastid RNA polymerase, is essential for early chloroplast development in rice

文献类型： 外文期刊

第一作者： Wang, Liwei

作者： Wang, Liwei;Wang, Chunming;Wang, Yihua;Niu, Mei;Zhou, Kunneng;Zhang, Huan;Jiang, Ling;Wan, Jianmin;Ren, Yulong;Lin, Qibing;Wu, Fuqing;Cheng, Zhijun;Wang, Jiulin;Zhang, Xin;Guo, Xiuping;Lei, Cailin;Wang, Jie;Zhu, Shanshan;Zhao, Zhichao;Wan, Jianmin

作者机构：

关键词： Nuclear chloroplast interaction;Oryza sativa;PEP;pTAC3;WSL3

期刊名称：PLANT MOLECULAR BIOLOGY （ 影响因子：4.076； 五年影响因子：4.89 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Plastid-encoded plastid RNA polymerase (PEP), a dominant RNA polymerase in mature chloroplasts, consists of core subunits and peripheral subunits. Despite the importance of the peripheral subunits in control of PEP activity it is unclear how they interact with one another to exert physiological effects on chloroplast development and plant growth, especially in rice. Here, we report a mutant, designated wsl3 that lacks a peripheral subunit in rice. We isolated the WSL3 gene encoding an essential peripheral subunit of rice PEP complex, OsPAP1/OspTAC3 by map-based cloning, and verified its function by complementation analysis. The wsl3 mutant showed a typical expression pattern of plastid-encoded genes, suggesting that PEP activity was impaired. Using immunofluorescent labeling and immunoblotting, we found that WSL3 was localized to the chloroplast and associated with the nucleoid. In addition, we demonstrated that WSL3 interacted with PEP subunits in Y2H, BiFC and pull-down experiments. Furthermore, a cpDNA IP assay revealed that WSL3 was associated with the PEP complex during the entire transcription process. We provide evidence suggesting that WSL3 is essential for early chloroplast development by interacting with subunits of the PEP complex.

分类号： Q946

  • 相关文献

[1]Comparison of Five Endogenous Reference Genes for Specific PCR Detection and Quantification of Brassica napus. Wu, Gang,Zhang, Li,Wu, Yuhua,Cao, Yinglong,Lu, Changming.

[2]Effects of elevated air temperature on physiological characteristics of flag leaves and grain yield in rice. Liu, Qi-Hua,Wu, Xiu,Ma, Jia-Qing,Zhou, Xue-Biao,Li, Tian. 2013

[3]Detection of epistatic interactions of three QTLs for heading date in rice using single segment substitution lines. Ding, Han-Feng,Liu, Xu,Li, Run-Fang,Wang, Wen-Ying,Zhang, Y.,Zhang, Xiao-Dong,Yao, Fang-Yin,Li, Guang-Xian,Jiang, Ming-Song,Ding, Han-Feng.

[4]Virus resistance obtained in transgenic tobacco and rice by RNA interference using promoters with distinct activity. Zhang, C.,Song, Y.,Jiang, F.,Jiang, Y.,Zhu, C.,Wen, F.,Li, G..

[5]Application of silicon fertilizer affects nutritional quality of rice. Liu, Qihua,Zhou, Xuebiao,Sun, Zhaowen. 2017

[6]Frequency distribution of sensitivity of Ustilaginoidea virens to four EBI fungicides, prochloraz, difenoconazole, propiconazole and tebuconazole, and their efficacy in controlling rice false smut in Anhui Province of China. Chen, Yu,Zhang, Yong,Yang, Xue,Wang, Wen-Xiang,Zhang, Ai-Fang,Gao, Tong-Chun,Chen, Yu,Zhang, Yong,Yang, Xue,Wang, Wen-Xiang,Zhang, Ai-Fang,Gao, Tong-Chun,Yao, Jian,Li, Yun-Fei,Chen, Yu,Zhang, Yong,Yang, Xue,Wang, Wen-Xiang,Zhang, Ai-Fang,Gao, Tong-Chun. 2013

[7]Cultivar group of rice cultivated in Caoxieshan site (B.P.6000 similar to present) determined by the morphology of plant opals and its historical change. Wang, CL,Udatsu, T,Tang, LH,Zhou, JS,Zheng, YF,Sasaki, A,Yanagisawa, K,Fujiwara, H. 1998

[8]A Young Seedling Stripe2 phenotype in rice is caused by mutation of a chloroplast-localized nucleoside diphosphate kinase 2 required for chloroplast biogenesis. Zhou, Kunneng,Xia, Jiafa,Wang, Yuanlei,Ma, Tingchen,Li, Zefu. 2017

[9]Characterization and identification of cold tolerant near-isogenic lines in rice. Zhou, Lei,Hu, Guanglong,Pan, Yinghua,Zhang, Hongliang,Li, Jinjie,Li, Zichao,Zhou, Lei,Hu, Guanglong,Pan, Yinghua,Zhang, Hongliang,Li, Jinjie,Li, Zichao,Zeng, Yawen,Yang, Shuming,Zhou, Lei,You, Aiqing,Hu, Guanglong. 2012

[10]Molecular mapping of a pollen killer gene S29(t) in Oryza Glaberrima and co-linear analysis with S22 in O-Glumaepatula. Hu, Fengyi,Xu, Peng,Deng, Xianneng,Zhou, Jiawu,Li, Jing,Tao, Dayun. 2006

[11]Involvement of Polyamine Oxidase-Produced Hydrogen Peroxide during Coleorhiza-Limited Germination of Rice Seeds. Chen, Bing-Xian,Li, Wen-Yan,Gao, Yin-Tao,Chen, Zhong-Jian,Zhang, Wei-Na,Liu, Qin-Jian,Chen, Zhuang,Liu, Jun. 2016

[12]Reactive Oxygen Species Generated by NADPH Oxidases Promote Radicle Protrusion and Root Elongation during Rice Seed Germination. Li, Wen-Yan,Chen, Bing-Xian,Chen, Zhong-Jian,Gao, Yin-Tao,Chen, Zhuang,Liu, Jun. 2017

[13]Comparison of high-yield rice in tropical and subtropical environments - I. Determinants of grain and dry matter yields. Ying, JF,Peng, SB,He, QR,Yang, H,Yang, CD,Visperas, RM,Cassman, KG. 1998

[14]Genetic Diversity and Structure of New Inbred Rice Cultivars in China. Xu Qun,Wang Cai-hong,Yu Han-yong,Yuan Xiao-ping,Wang Yi-ping,Feng Yue,Tang Sheng-xiang,Wei Xing-hua,Chen Hong. 2012

[15]Microarray-based gene expression analysis of strong seed dormancy in rice cv. N22 and less dormant mutant derivatives. Wu, Tao,Yang, Chunyan,Ding, Baoxu,Feng, Zhiming,Wang, Qian,He, Jun,Jiang, Ling,Wan, Jianmin,Wan, Jianmin,Tong, Jianhua,Xiao, Langtao. 2016

[16]Driving the expression of RAA1 with a drought-responsive promoter enhances root growth in rice, its accumulation of potassium and its tolerance to moisture stress. Chen, Guang,Li, Chaolei,Gao, Zhenyu,Zhang, Yu,Zhu, Li,Hu, Jiang,Ren, Deyong,Qian, Qian,Chen, Guang,Xu, Guohua. 2018

[17]Molecular dissection of the primary sink size and its related traits in rice. Xu, JL,Yu, SB,Luo, LJ,Zhong, DB,Mei, HW,Li, ZK. 2004

[18]Dissection of component QTL expression in yield formation in rice. Guo, LB,Xing, YZ,Mei, HW,Xu, CG,Shi, CH,Wu, P,Luo, LJ. 2005

[19]OPEN GLUME1: a key enzyme reducing the precursor of JA, participates in carbohydrate transport of lodicules during anthesis in rice. Li, Xiaohui,Wang, Yihua,Duan, Erchao,Zhou, Kunneng,Lin, Qiuyun,Wang, Di,Wang, Yunlong,Long, Wuhua,Zhao, Zhigang,Jiang, Ling,Wang, Chunming,Wan, Jianmin,Cheng, Zhijun,Lei, Cailin,Zhang, Xin,Guo, Xiuping,Wang, Jiulin,Wu, Chuanyin,Wan, Jianmin,Qi, Qi. 2018

[20]Single base substitution in OsCDC48 is responsible for premature senescence and death phenotype in rice. Huang, Qi-Na,Shi, Yong-Feng,Zhang, Xiao-Bo,Song, Li-Xin,Feng, Bao-Hua,Wang, Hui-Mei,Xu, Xia,Li, Xiao-Hong,Guo, Dan,Wu, Jian-Li,Song, Li-Xin. 2016

作者其他论文 更多>>

微信小程序