Nutritional functions of the funiculus in Brassica napus seed maturation revealed by transcriptome and dynamic metabolite profile analyses

文献类型： 外文期刊

第一作者： Tan, Helin

作者： Tan, Helin;Xiang, Xiaoe;Tang, Jie;Wang, Xingchun;Wang, Xingchun

作者机构：

关键词： Brassica napus;Funiculus;Nutrient transport;Metabolite profile;Transcriptome

期刊名称：PLANT MOLECULAR BIOLOGY （ 影响因子：4.076； 五年影响因子：4.89 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The funiculus provides the sole channel of communication between the seed and the parent plant; however, little is known about its role in nutrient supply during seed maturation. Here, we investigated the dynamic metabolite profiles of the funiculus during seed maturation in Brassica napus. The funiculus was fully developed at 21 days after flowering (DAF), but the levels of nutrients, including carbohydrates, fatty acids, and amino acids, increased rapidly from 21 to 35 DAF. Orthogonal partial least squares discriminant analysis and correlation analysis identified 37 metabolites that correlated closely with seed fresh weight. To determine the influence of silique wall photosynthesis on the metabolites in the funiculus, we also covered the siliques of intact plants with aluminum foil; in these plants, the funiculus and silique wall had lower metabolite levels, compared with control. RNA-sequencing analysis of the funiculi in the dark-treated and light-exposed siliques showed that the expression of genes encoding nutrient transporters significantly increased in the funiculi in the dark-treated siliques. Furthermore, the transcripts encoding primary metabolic enzymes for amino acid synthesis, fatty acid synthesis and triacylglycerol assembly, and sucrose-starch metabolism, were also markedly up-regulated, despite the decline in metabolite levels of funiculi in the dark-treated silique. These results provide new insights into funiculus function in seed growth and synthesis of storage reserves in seeds, at the metabolic and transcriptional levels. The identification of these metabolites and genes also provides useful information for creating genetically enhanced oilseed crops with improved seed properties.

分类号： Q946

  • 相关文献

[1]Transcriptome Analysis of Stem and Globally Comparison with Other Tissues in Brassica napus. Miao, Liyun,Zhang, Libin,Raboanatahiry, Nadia,Fu, Chunhua,Li, Maoteng,Miao, Liyun,Xiang, Jun,Gan, Jianping,Li, Maoteng,Lu, Guangyuan,Zhang, Xuekun. 2016

[2]Transcriptomic Analysis of Seed Coats in Yellow-Seeded Brassica napus Reveals Novel Genes That Influence Proanthocyanidin Biosynthesis. Hong, Meiyan,Hu, Kaining,Tian, Tiantian,Li, Xia,Chen, Li,Zhang, Yan,Yi, Bin,Wen, Jing,Ma, Chaozhi,Shen, Jinxiong,Fu, Tingdong,Tu, Jinxing,Li, Xia,Chen, Li,Zhang, Yan. 2017

[3]Production of red-flowered oilseed rape via the ectopic expression of Orychophragmus violaceus OvPAP2. Fu, Wenqin,Chen, Daozong,Pan, Qi,Li, Fengfeng,Ge, Xianhong,Li, Zaiyun,Zhao, Zhigang. 2018

[4]Transcriptome analysis of Brassica napus pod using RNA-Seq and identification of lipid-related candidate genes. Xu, Hai-Ming,Kong, Xiang-Dong,Chen, Fei,Huang, Ji-Xiang,Zhao, Jian-Yi,Lou, Xiang-Yang. 2015

[5]Comparative Analysis of the Brassica napus Root and Leaf Transcript Profiling in Response to Drought Stress. Liu, Chunqing,Zhang, Ka,An, Hong,Hu, Kaining,Wen, Jing,Shen, Jinxiong,Ma, Chaozhi,Yi, Bin,Tu, Jinxing,Fu, Tingdong,Zhang, Xuekun. 2015

[6]Analysis of transcriptional and epigenetic changes in hybrid vigor of allopolyploid Brassica napus uncovers key roles for small RNAs. Shen, Yifei,Sun, Shuo,Shen, Enhui,Ye, Chu-Yu,Fan, Longjiang,Shen, Yifei,Sun, Shuo,Shen, Enhui,Ye, Chu-Yu,Fan, Longjiang,Hua, Shuijin,Cai, Daguang,Timko, Michael P.,Zhu, Qian-Hao.

[7]Cello-oligosaccharide influences intestinal microflora, mucosal architecture and nutrient transport in weaned pigs. Jiao, L. F.,Song, Z. H.,Ke, Y. L.,Xiao, K.,Hu, C. H.,Shi, B..

[8]A collection of 10,096 indica rice full-length cDNAs reveals highly expressed sequence divergence between Oryza sativa indica and japonica subspecies. Liu, Xiaohui,Lu, Tingting,Yu, Shuliang,Li, Ying,Huang, Yuchen,Huang, Tao,Zhang, Lei,Zhu, Jingjie,Zhao, Qiang,Fan, Danlin,Mu, Jie,Shangguan, Yingying,Feng, Qi,Guan, Jianping,Ying, Kai,Zhang, Yu,Lin, Zhixin,Sun, Zongxiu,Qian, Qian,Lu, Yuping,Han, Bin.

[9]Expression of Aquaporin BnPIP-like Gene from Rapeseed (Brassica napus) Enhances Salt Resistance in Yeast (Pichia pastoris). Li, Hao-Jie,Zhang, Jin-Fang,Cui, Cheng,Jiang, Jun,Zheng, Ben-Chuan,Jiang, Liang-Cai,Tan, Hao,Zhang, Bi. 2016

[10]Comparative Transcriptomic Analysis of Two Brassica napus Near-Isogenic Lines Reveals a Network of Genes That Influences Seed Oil Accumulation. Wang, Jingxue,Li, Chen,Yuan, Ling,Singh, Sanjay K.,Pattanaik, Sitakanta,Yuan, Ling,Du, Chunfang,Fan, Jianchun. 2016

[11]PHOTOSYNTHESIS AND ANTIOXIDANT RESPONSE TO WINTER RAPESEED (BRASSICA NAPUS L.) AS AFFECTED BY BORON. Hossain, Md Faruque,Pan Shenggang,Duan Meiyang,Mo Zhaowen,Karbo, Maurice Baimba,Tang Xiangru,Hossain, Md Faruque,Pan Shenggang,Duan Meiyang,Mo Zhaowen,Karbo, Maurice Baimba,Tang Xiangru,Hossain, Md Faruque,Bano, Asghari. 2015

[12]Diversity and biocontrol potential of endophytic fungi in Brassica napus. Zhang, Jing,Yang, Long,Jiang, Daohong,Li, Guoqing,Zhang, Qinghua,Zhang, Jing,Yang, Long,Jiang, Daohong,Li, Guoqing,Zhang, Lei,Chen, Weidong. 2014

[13]New insights into the genetic networks affecting seed fatty acid concentrations in Brassica napus. Wang, Xiaodong,Yin, Yongtai,Gan, Lu,Yu, Longjiang,Li, Maoteng,Long, Yan,Zhang, Chunyu,Meng, Jinling,Long, Yan,Wang, Xiaodong,Liu, Liezhao. 2015

[14]A Study on Triacylglycerol Composition and the Structure of High-Oleic Rapeseed Oil. Guan, Mei,Xiong, Xinghua,Li, Xun,Guan, Chunyun,Chen, Hong,Lu, Xin,Huang, Fenghong. 2016

[15]Genetic diversity of Brassica carinata with emphasis on the interspecific crossability with B-rapa. Jiang, Y.,Tian, E.,Li, R.,Chen, L.,Meng, J.. 2007

[16]Morphological Structure and Transcriptome Comparison of the Cytoplasmic Male Sterility Line in Brassica napus (SaNa-1A) Derived from Somatic Hybridization and Its Maintainer Line SaNa-1B. Du, Kun,Liu, Qier,Wu, Xinyue,Jiang, Jinjin,Wu, Jian,Fang, Yujie,Wang, Youping,Li, Aimin. 2016

[17]Quantitative trait loci analysis and genome-wide comparison for silique related traits in Brassica napus. Wang, Xiaodong,Chen, Li,Chao, Hongbo,Li, Maoteng,Wang, Xiaodong,Chen, Li,Xiang, Jun,Gan, Jianping,Wang, Aina,Wang, Hao,Tian, Jianhua,Zhao, Xiaoping,Zhao, Yajun,Zhao, Weiguo. 2016

[18]Genome-Wide Association and Transcriptome Analyses Reveal Candidate Genes Underlying Yield-determining Traits in Brassica napus. Lu, Kun,Peng, Liu,Zhang, Chao,Lu, Junhua,Yang, Bo,Xiao, Zhongchun,Liang, Ying,Xu, Xingfu,Qu, Cunmin,Zhang, Kai,Liu, Liezhao,Li, Jiana,Peng, Liu,Zhang, Chao,Zhu, Qinlong,Fu, Minglian,Yuan, Xiaoyan. 2017

[19]SCAR and RAPD markers associated with 18-carbon fatty acids in rapeseed, Brassica napus. Hu, J,Li, G,Struss, D,Quiros, CF. 1999

[20]Genetic diversity among populations and breeding lines from recurrent selection in Brassica napus as revealed by RAPD markers. Yuan, M,Zhou, Y,Liu, D. 2004

作者其他论文 更多>>

微信小程序