Morphological and molecular characterization of the second backcross progenies of Ogu-CMS Chinese kale and rapeseed

文献类型： 外文期刊

第一作者： Yu, Hai-long

作者： Yu, Hai-long;Zhang, Lu-gang;Fang, Zhi-yuan;Yu, Hai-long;Zhang, Lu-gang;Fang, Zhi-yuan;Yu, Hai-long;Li, Zhi-yuan;Yang, Li-mei;Liu, Yu-mei;Zhuang, Mu;Lv, Hong-hao;Li, Zhan-sheng;Han, Feng-qing;Liu, Xiao-ping;Fang, Zhi-yuan;Zhang, Yang-yong;Yu, Hai-long;Li, Zhi-yuan;Yang, Li-mei;Liu, Yu-mei;Zhuang, Mu;Lv, Hong-hao;Li, Zhan-sheng;Han, Feng-qing;Liu, Xiao-ping;Fang, Zhi-yuan;Zhang, Yang-yong

作者机构：

关键词： Backcross;Fertility-restored;Improved seed-setting;Genetic background;Brassica oleracea L. var. alboglabra

期刊名称：EUPHYTICA （ 影响因子：1.895； 五年影响因子：2.181 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Ogura cytoplasmic male sterility (OguCMS) is widely used in the production of commercial hybrids of Brassica oleracea. However, the widespread application of the Ogu-CMS system in B. oleracea has hindered the germplasm innovation of Ogu-CMS resources due to the lack of a natural restorer line. Previously, the Ogu-CMS fertility-restored interspecific hybrids between rapeseed 15Y403 (2n = 38, AACC) and Chinese kale JL1 (2n = 18, CC) have been successfully produced. However, these progenies, which still contained a large proportion of rapeseed genomic components, showed poor fertility and a low seed setting rate under natural pollination. To improve fertility and seed setting, a successive backcross with JL1 was performed to produce BC2 progenies. Screening with the Rfo-specific marker, five individuals harboring the Rfo gene were identified among 98 BC2 progenies. These five individuals underwent background marker screening and an evaluation of agronomic traits and fertility. One individual (code: 15Q23) was identified with higher pollen viability, better seed setting under natural pollination, and a closer genetic background to the parent Chinese kale JL1. Many morphological traits showed no significant differences (P < 0.05) between 15Q23 and the backcross parent JL1. However, the average seed setting of 15Q23 under natural pollination was 0.72 seeds per pod, which was 50 times higher than that of BC1 progenies, and the average pollen viability was 87.07%, which was significantly better than that of the F-1 and BC1 plants (P < 0.01). The genetic background of 15Q23 was more closer to the parent JL1 than that of BC1 plants and another BC2 fertility-restored individual, with 82% of the polymorphic alleles being the same as those of the parent Chinese kale JL1. Thus, the individual 15Q23 could be used as a donor plant for further backcrosses. This study lays the foundation for the development of Ogu-CMS restorer material in B. oleracea.

分类号： S3

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