Elucidating Cultivar Differences in Plant Regeneration Ability in an Apple Anther Culture

文献类型： 外文期刊

第一作者： Zhang, Chunfen

作者： Zhang, Chunfen;Zhang, Chunfen;Cao, Qiufen;Sato, Sota;Tsukuni, Tatsuro;Yoshikawa, Nobuyuki;Mimida, Naozumi;Takagishi, Kaori;Komori, Sadao;Sato, Mamoru;Okada, Hatsuhiko;Yamamoto, Toshiya;Wada, Masato;Matsumoto, Shogo;Watanabe, Manabu;Sato, Sota;Tsukuni, Tatsuro;Mimida, Naozumi;Takagishi, Kaori

作者机构：

关键词： acclimatization;callus formation;embryo formation;Malus x domestica Borkh;shoot formation

期刊名称：HORTICULTURE JOURNAL （ 影响因子：0.959； 五年影响因子：1.115 ）

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收录情况： SCI

摘要： To select cultivars to produce doubled haploids (DHs) efficiently in an anther culture, a total of 28 apple cultivars (25 cultivars and 3 rootstock cultivars) were investigated to assess their callus formation rate, embryo formation rate, shoot formation rate from embryo, and efficiency of shoot multiplication and acclimatization. The callus formation rates of 'Red Astrachan', 'Kinser, 'Tsugaru', 'Golden Delicious' ('GD'), 'American Summer Pearmain' ('ASP'), 'Gala', 'Rome Beauty', and 'Jonathan' were high, but 'JM2' and 'King of Tompkins' formed no callus. Regarding the embryo formation rates, 'ASP', 'Rome Beauty', 'M.9', and 'Starking Delicious' ('SD') were high, but 'G-D', 'JM2', 'King of Tompkins', 'Rails Janet', and 'Smith's Cider' formed no embryo. Regarding the shoot formation rate from embryos, 'Senshu', 'Tsugaru', and 'Sansa' showed high values, but 'Fuji', 'Jonathan', and 'Wijcik' did not form shoots at all. The rate of shoot formation from anthers showed a high- low order of 'Senshu', 'SD', 'ASP', and 'Tsugaru'. Multiplication and acclimatization of the shoots were not easy. Many individuals died. Eventually, only 'Senshu' and 'SD' acclimatized. Therefore, cultivars that produced DHs efficiently were 'Senshu' and 'SD'. The results indicated that all processes of "embryo formation", "shoot induction", and "shoot multiplication and acclimatization" are important for obtaining DHs in apple anther cultures.

分类号： S6

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