Effect of chicken leptin recptor short hairpin RNA on expression of JAK2, STAT3, SOCS3 and CPT1 genes in chicken preadipocytes
文献类型: 外文期刊
第一作者: Ban, Qian
作者: Ban, Qian;Cheng, Fei;Liu, Dahai;Hui, Wenqiao;Liu, Xiaojun;Ban, Qian;Hui, Wenqiao;Liu, Xiaojun
作者机构:
关键词: chicken;LEPR;preadipocytes;RNAi
期刊名称:ANIMAL SCIENCE JOURNAL ( 影响因子:1.749; 五年影响因子:1.909 )
ISSN:
年卷期:
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收录情况: SCI
摘要: In this study, we detect depressive effect on leptin receptor (LEPR) by LEPR-specific short hairpin RNA (shRNA) expression plasmids in chicken preadipocytes, and effect on messenger RNA (mRNA) expression levels of genes related to signal transduction, including JAK2, STAT3, SOCS3 as well as CPT1, which is associated with fatty acid metabolism. shRNA expression vectors targeting LEPR were constructed and transfected into chicken preadipocytes. The transfection efficiency was evaluated by fluorescence microscopy. Real-time PCR was used to detect its effect on mRNA expression levels of JAK2, STAT3, SOCS3 and CPT1. Results showed that LEPR mRNA was knocked down by 99% (P<0.01) after transfection for 72h. In the knockdown preadipocytes, the mRNA levels of JAK2 and CPT1 were down-regulated by 47.56% (P<0.01) and 42.26% (P<0.05), respectively; while expression of STAT3 and SOCS3 increased 7.72-fold (P<0.01), 1.71-fold (P<0.01), respectively. It is concluded that knockdown of LEPR influences mRNA expression of its down-stream genes, suggesting that chicken LEPR play a certain role in regulating genes in the complicated gene network of preadipocytes.
分类号: S8
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