Development of a reverse transcription loop-mediated isothermal amplification assay for the rapid detection of Pepper mottle virus

文献类型： 外文期刊

第一作者： Zhang, Deyong

作者： Zhang, Deyong;Zheng, Limin;Peng, Jing;Zhang, Songbai;Liu, Yong;Li, Fan

作者机构：

关键词： field disease diagnosis;PepMoV;Potyvirus;RT-LAMP assay;RT-PCR

期刊名称：CANADIAN JOURNAL OF PLANT PATHOLOGY （ 影响因子：2.442； 五年影响因子：1.993 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Pepper mottle virus (PepMoV) is a widespread threat to vegetable crop production in the USA and south-east Asia. We describe the development of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay to detect PepMoV. The RT-LAMP assay was based on a set of four primers that match a specific region of the coat protein gene in the PepMoV genome. The detection limit of conventional RT-PCR detection was 1.47 x 10(-4) mu g mu L-1 of cDNA, whereas RT-LAMP was 10 times more sensitive. Using RT-LAMP, PepMoV detection was also highly specific, showing no cross-activity with four other potyviruses. Sixty-nine field samples collected from symptomatic pepper plants growing in five South China provinces were tested for the presence of PepMoV infection by performing an RT-LAMP assay as well as a conventional RT-PCR. Both methods detected PepMoV in 18 samples, demonstrating that the PepMoV-specific RT-LAMP assay could be a useful alternative tool for the diagnosis and epidemiological surveillance of PepMoV infections. The RT-LAMP assay also has the advantages that it can be performed in a low-tech environment and is quicker and cheaper to perform than conventional RT-PCR.

分类号： S4

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