Effects of dietary -ketoglutarate supplementation onthe antioxidant defense system and HSP 70 and HSP 90 gene expression of hybrid sturgeon Acipenser schrenckii fxA.baerii o exposed to ammonia-N stress

文献类型： 外文期刊

第一作者： Wang, Liansheng

作者： Wang, Liansheng;Xu, Qiyou;Wang, Chang'an;Li, Jinnan;Chen, Di;Zhao, Zhigang;Luo, Liang;Du, Xue;Chen, Di

作者机构：

关键词： alpha-ketoglutarate;ammonia-N stress;hybrid sturgeon;antioxidant;heat shock protein

期刊名称：AQUACULTURE RESEARCH （ 影响因子：2.082； 五年影响因子：2.415 ）

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收录情况： SCI

摘要： This study was conducted to determine the effects of dietary -ketoglutarate (AKG) supplementation on the antioxidant defense system and gene expression of heat shock protein (HSP) 70 and HSP 90 in hybrid sturgeons Acipenser schrenckii fxA.baerii o exposed to ammonia-N stress. A 2x3 factorial experiment was arranged, in which each diet (0%, 1% AKG) was randomly assigned to 0.25 (control) 5 and 10mgL(-1) ammonia-N groups with three replicate aquaria for each 72h. The 10mgL(-1) ammonia-N significantly increased serum ammonia concentrations and intestinal Gln concentrations and GS activity compared with the 0.25 or 5mgL(-1) ammonia-N groups. The intestinal Gln concentration and GS activity increased, and the serum ammonia concentration decreased, in fish given dietary supplementation of 1.0% AKG compared with fish given diets without AKG. Superoxide dismutase (SOD) and glutathione peroxidase (GPx) activity in serum, gills and intestines decreased when fish were exposed to 5 or 10mgL(-1) ammonia-N, and their activity increased in fish given diets with 1% AKG. Catalase in the serum and gills decreased when fish were exposed to 5 or 10mgL(-1) ammonia-N and increased in fish given diets with 1% AKG. The 10mgL(-1) ammonia-N or 1% AKG supplementation increased HSP 70 and HSP 90 gene expression in the liver. The increased activity of antioxidant enzymes, and increased HSP 70 andHSP 90 gene expression in fish fed diets containing 1% AKG suggested higher tolerance to ammonia-N stress.

分类号： S9

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