A coagulase-negative and non-haemolytic strain of Staphylococcus aureus for investigating the roles of SrtA in a murine model of bloodstream infection

文献类型： 外文期刊

第一作者： Wang, Lin

作者： Wang, Lin;Deng, Xuming;Bi, Chongwei;Wang, Tiedong;Hu, Jinping;Liu, Bingrun;Zhong, Xiaobo;Wang, Dacheng;Xiang, Hua;Cai, Hongjun;Chen, Fuguang

作者机构：

关键词： Sortase A;Staphylococcus aureus;renal abscess;mouse model

期刊名称：PATHOGENS AND DISEASE （ 影响因子：3.166； 五年影响因子：3.081 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Sortase A (SrtA) is a cysteine transpeptidase and virulence factor from Staphylococcus aureus (S. aureus) that catalyses the attachment and display of surface proteins on the cell wall, thereby mediating bacterial adhesion to host tissues, host-cell entry and evasion of the immune response. As a result, SrtA has become an important target in the development of therapies for S. aureus infections. In this study, we used the new reference strain S. aureus Newman D2C to investigate the role of SrtA in a murine model of bloodstream infection, when the impact of coagulase and haemolysin is excluded. The results suggested that deletion of SrtA reduced the bacterial burden on the heart, liver and kidneys by blunting the host proinflammatory cytokine response at an early point in infection. Kidneys, but not heart or liver, formed abscesses on the sixth day following non-lethal infection, and this effect was diminished by SrtA mutation. These findings indicate that SrtA is a determining virulence factor in lethality and formation of renal abscesses in mice followed by S. aureus bloodstream infection. We have thus established a convenient in vitro and mouse model for developing SrtA-targeted therapeutic strategies.

分类号： R37

  • 相关文献

[1]Quercitrin, an Inhibitor of Sortase A, Interferes with the Adhesion of Staphylococcal aureus. Liu, Bingrun,Chen, Fuguang,Bi, Chongwei,Zhong, Xiaobo,Cai, Hongjun,Wang, Dacheng,Chen, Fuguang,Wang, Lin,Deng, Xuming,Niu, Xiaodi.

[2]Serum Metabolic Profiling of Oocyst-Induced Toxoplasma gondii Acute and Chronic Infections in Mice Using Mass-Spectrometry. Zhou, Chun-Xue,Chen, Xiao-Qing,Zhu, Xing-Quan,Zhou, Chun-Xue,He, Shen-Yi,Cong, Wei,Chen, Xiao-Qing,Elsheikha, Hany M.. 2018

[3]Characterization of a highly pathogenic H5N1 influenza virus derived from bar-headed geese in China. Zhou, JY,Shen, HG,Chen, HX,Tong, GZ,Liao, M,Yang, HC,Liu, JX.

[4]A PB1 T296R substitution enhance polymerase activity and confer a virulent phenotype to a 2009 pandemic H1N1 influenza virus in mice. Yu, Zhijun,Qin, Chuan,Xia, Xianzhu,Yu, Zhijun,Qin, Chuan,Xia, Xianzhu,Yu, Zhijun,Sun, Weiyang,Zhang, Xinghai,Li, Yuanguo,Wang, Tiecheng,Wang, Hualei,Xin, Yue,Li, Xue,Zhang, Kun,Huang, Jing,Yang, Songtao,Gao, Yuwei,Xia, Xianzhu,Cheng, Kaihui,Zhang, Qianyi,Chen, Hualan,Wilker, Peter R.,Yue, Donghui,Gao, Yuwei,Xia, Xianzhu,Xia, Xianzhu.

[5]An adapted duck Tembusu virus induces systemic infection and mediates antibody-dependent disease severity in mice. Liu, Zongliang,Ji, Yanhong,Huang, Xiaohui,Fu, Yuguang,Cai, Xuepeng,Zhu, Qiyun,Wei, Jianzhong.

[6]Assessment of antibiotic susceptibilities, genotypic characteristics and biofilm-forming abilities of Staphylococcus aureus and Salmonella Typhimurium. Xu, Hua,Ahn, Juhee,Xu, Hua,He, Xinlong,Ahn, Juhee. 2011

[7]Penicillin-resistant characterization of Staphylococcus aureus isolated from bovine mastitis in Gansu, China. Yang Feng,Liu Long-Hai,Wang Ling,Wang Xu-rong,Li Xin-Pu,Jin-yin, Luo,Zhang Zhe,Zhang Shi-dong,Yan Zuo-ting,Li Hong-sheng. 2017

[8]Design and pharmacodynamics of recombinant NZ2114 histidine mutants with improved activity against methicillin-resistant Staphylococcus aureus. Chen, Huixian,Feng, Xingjun,Chen, Huixian,Mao, Ruoyu,Teng, Da,Wang, Xiumin,Hao, Ya,Wang, Jianhua,Chen, Huixian,Mao, Ruoyu,Teng, Da,Wang, Xiumin,Hao, Ya,Wang, Jianhua. 2017

[9]Study on the Hemolysin Phenotype and the Genetype Distribution of Staphyloccocus aureus Caused Bovine Mastitis in Shandong Dairy Farms. Wang Fei,Yang Hongjun,He Hong-bin,Wang Changfa,Gao Yundong,Zhong Qifeng,Wang Fei,Wang Xiaohong,Zeng Yanjun. 2011

[10]Selective Cultivation and Rapid Detection of Staphylococcus aureus by Computer Vision. Wang, Yong,Yin, Yongguang,Zhang, Chaonan,Wang, Yong. 2014

[11]Prevalence of blaZ gene and other virulence genes in penicillin-resistant Staphylococcus aureus isolated from bovine mastitis cases in Gansu, China. Yang, Feng,Wang, Xurong,Wang, Ling,Xiao, Min,Li, Xinpu,Luo, Jinyin,Zhang, Shidong,Li, Hongsheng,Wang, Qi.

[12]Design and recombination expression of a novel plectasin-derived peptide MP1106 and its properties against Staphylococcus aureus. Cao, Xintao,Zhang, Yong,Mao, Ruoyu,Teng, Da,Wang, Xiumin,Wang, Jianhua,Cao, Xintao,Zhang, Yong,Mao, Ruoyu,Teng, Da,Wang, Xiumin,Wang, Jianhua.

[13]Combination of microbiological, spectroscopic and molecular docking techniques to study the antibacterial mechanism of thymol against Staphylococcus aureus: membrane damage and genomic DNA binding. Wang, Lang-Hong,Zhang, Zhi-Hong,Zeng, Xin-An,Wang, Lang-Hong,Zhang, Zhi-Hong,Zeng, Xin-An,Gong, De-Ming,Wang, Man-Sheng.

[14]Morin hydrate attenuates Staphylococcus aureus virulence by inhibiting the self-assembly of alpha-hemolysin. Wang, J.,Zhou, X.,Liu, S.,Li, G.,Shi, L.,Dong, J.,Li, W.,Deng, X.,Niu, X.,Dong, J..

[15]Effective Antimicrobial Activity of Plectasin-Derived Antimicrobial Peptides against Staphylococcus aureus Infection in Mammary Glands. Li, Lianbin,Gao, Yuqi,Zhao, Xin,Wang, Liangliang,Wang, Jianhua,Zhao, Xin. 2017

[16]Genetic characterization of antimicrobial resistance in Staphylococcus aureus isolated from bovine mastitis cases in Northwest China. Yang Feng,Wang Xu-rong,Wang Ling,Li Xin-pu,Luo Jin-yin,Zhang Shi-dong,Li Hong-sheng,Wang Qi. 2016

[17]Staphylococcus aureus infection of intestinal epithelial cells induces human umbilical cord-derived mesenchymal stem cell migration. Li, Yang,Liu, Ming-yuan,Wang, Xue-lin,Han, Wen-yu,Liu, Ya-hui,Suo, Jian,Li, Zhi-jie,Li, Ya-gang,Jin, Hu. 2013

[18]Antimicrobial Activity Investigation on Wuyiencin Fractions of Different Polarity. Cui, Zengjie,Zhang, Kecheng,Sun, Lei,Cheng, Yan,Tan, Beibei,She, Gaimei,Lin, Yinni. 2010

[19]Prevalence, antimicrobial susceptibility, and molecular characterization of Staphylococcus aureus isolated from dairy herds in northern China. Liu, Huimin,Li, Songli,Meng, Lu,Dong, Lei,Zhao, Shengguo,Lan, Xinyi,Wang, Jiaqi,Zheng, Nan,Liu, Huimin,Li, Songli,Meng, Lu,Dong, Lei,Zhao, Shengguo,Lan, Xinyi,Wang, Jiaqi,Zheng, Nan. 2017

[20]Bovine mastitis Staphylococcus aureus: Antibiotic susceptibility profile, resistance genes and molecular typing of methicillin-resistant and methicillin-sensitive strains in China. Wang, Dengfeng,Ali, Tariq,Lv, Yanli,Han, Bo,Wang, Dengfeng,Wang, Zhicai,Wu, Jianyong,Li, Jianjun,Yan, Zuoting.

作者其他论文 更多>>

微信小程序