Cloning, expression, and purification of an antiviral protein from Pseudomonas fluorescens CZ and its antagonistic activity against tobacco mosaic virus

文献类型: 外文期刊

第一作者: Shen, Lili

作者: Shen, Lili;Jiao, Yubing;Wu, Yuanhua;Shen, Lili;Li, Fangfang;Sun, Hangjun;Wang, Fenglong

作者机构:

关键词: Pseudomonas fluorescens;antiviral protein;expression and purification;antagonistic activity

期刊名称:BIOCONTROL SCIENCE AND TECHNOLOGY ( 影响因子:1.665; 五年影响因子:1.616 )

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收录情况: SCI

摘要: The antiviral alkaline phosphatase L (AapL) protein of Pseudomonas fluorescens CZ was expressed in Escherichia coli, purified using the Ni-NTA column, and its antiviral activity against tobacco mosaic virus (TMV)-infected Nicotiana was tested. The recombinant protein (360 mu g/mL) showed stability and most effective suppression in vitro (94.85%) against TMV.

分类号: S476

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