Bombyx mori Serpin6 regulates prophenoloxidase activity and the expression of antimicrobial proteins

文献类型： 外文期刊

第一作者： Li, Bing

作者： Li, Bing;Yu, Hai-Zhong;Ye, Chong-Jun;Ma, Yan;Xu, Jia-Ping;Li, Bing;Ye, Chong-Jun;Li, Xing;Fan, Tao;Chen, Fu-Sheng

作者机构：

关键词： Serpin6;Prophenoloxidase;Antimicrobial peptides;Bombyx mori

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN：

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收录情况： SCI

摘要： Serpins are a family of serine protease inhibitors that are found widely in insects. They play an important role in insect physiological responses, such as innate immunity and development. In this study, we obtained the Bombyx mori serpin6 (BmSerpin6) sequence from National Center for Biotechnology Information (NCBI) and the silkworm genome database (SilkDB). Reverse transcription PCR (RT-PCR) results showed that BmSerpin6 was expressed highly in hemocytes, the midgut, and the fat body. After challenging with Micrococcus luteus (Mi) and Serratia marcescens (Sm), the BmSerpin6 expression level was induced significantly. Transcript levels of gloverin2 and prophenoloxidase (PPO) activity were reduced significantly in the fat body and hemocytes after injecting the recombinant BmSerpin6 protein into silkworm larvae. A BmSerpin6 recombinant plasmid (BmSerpin6-pAC 5.1) was constructed successfully and transfected into Drosophila S2 cells, which resulted in significantly reduced expression of the drosomycin protein. These results indicated that BmSerpin6 might regulate silkworm immune responses. (C) 2017 Elsevier B.V. All rights reserved.

分类号： R394

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