Characterization of miRNAs responsive to exogenous ethylene in grapevine berries at whole genome level

文献类型： 外文期刊

第一作者： Zhao, Fanggui

作者： Zhao, Fanggui;Wang, Chen;Han, Jian;Zhu, Xudong;Li, Xiaopeng;Fang, Jinggui;Zhao, Fanggui;Wang, Xicheng

作者机构：

关键词： Grapevine;Berry;MicroRNAs;Exogenous ethylene;High-throughput sequencing;qRT-PCR

期刊名称：FUNCTIONAL & INTEGRATIVE GENOMICS （ 影响因子：3.41； 五年影响因子：3.616 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： MicroRNAs (miRNAs) are critical regulators of various biological and metabolic processes of plants. Numerous miRNAs and their functions have been identified and analyzed in many plants. However, till now, the involvement of miRNAs in the response of grapevine berries to ethylene has not been reported yet. Here, Solexa technology was employed to deeply sequence small RNA libraries constructed from grapevine berries treated with and without ethylene. A total of 124 known and 78 novel miRNAs were identified. Among these miRNAs, 162 miRNAs were clearly responsive to ethylene, with 55 downregulated, 59 upregulated, and 14 unchanged miRNAs detected only in the control. The other 35 miRNAs responsive to ethylene were induced by ethylene and detected only in the ethylene-treated grapevine materials. Expression analysis of 27 conserved and 26 novel miRNAs revealed that 13 conserved and 18 novel ones were regulated by ethylene during the whole development of grapevine berries. High-throughput sequencing and qRT-PCR assays revealed consistent results on the expression results of ethylene-responsive miRNAs. Moreover, 90 target genes for 34 novel miRNAs were predicted, most of which were involved in responses to various stresses, especially like exogenous ethylene treatment. The identified miRNAs may be mainly involved in grapevine berry development and response to various environmental conditions.

分类号： Q78`Q3

  • 相关文献

[1]Parsing the Regulatory Network between Small RNAs and Target Genesin Ethylene Pathway in Tomato. Wang, Yunxiang,Wang, Qing,Gao, Lipu,Zuo, Jinhua,Wang, Yunxiang,Wang, Qing,Gao, Lipu,Zuo, Jinhua,Wang, Yunxiang,Wang, Qing,Gao, Lipu,Zuo, Jinhua,Wang, Yunxiang,Wang, Qing,Gao, Lipu,Zuo, Jinhua,Zhu, Benzhong,Ju, Zheng,Luo, Yunbo. 2017

[2]Computational identification of microRNAs in peach expressed sequence tags and validation of their precise sequences by miR-RACE. Zhang, Yanping,Yu, Huaping,Han, Jian,Song, Changnian,Fang, Jinggui,Yu, Mingliang,Ma, Ruijuan.

[3]Microwave Energy Absorption Behavior of Foamed Berry Puree Under Microwave Drying Conditions. Zheng, Xianzhe,Liu, Chenghai,Sun, Jingkun,Liu, Bingxin,Zhang, Baohui,Lin, Zhen,Sun, Yu,Liu, Haijun,Wang, Yingkuan,Lin, Zhen,Liu, Haijun.

[4]Studying the Mechanism of Plasmopara viticola RxLR Effectors on Suppressing Plant Immunity. Li, Xinlong,Wu, Jiao,Zhang, Yali,Lu, Jiang,Yin, Ling. 2016

[5]Effects of root restriction on nitrogen and gene expression levels in nitrogen metabolism in Jumeigui grapevines (Vitis vinifera L.xVitis labrusca L). Li Jie-fa,Wang Bo,Wang Lei,Zhang Cai-xi,Xu Wen-ping,Wang Shi-ping,Zhu Li-na,Bai Yang. 2015

[6]Mycosphere Notes 102-168: Saprotrophic fungi on Vitis in China, Italy, Russia and Thailand. Jayawardena, R. S.,Chethana, K. W. T.,Dissanayake, A. J.,Manawasinghe, I. S.,Li, X. H.,Liu, M.,Zhang, W.,Yan, J. Y.,Jayawardena, R. S.,Hyde, K. D.,Chethana, K. W. T.,Daranagama, D. A.,Dissanayake, A. J.,Goonasekara, I. D.,Manawasinghe, I. S.,Mapook, A.,Jayasiri, S. C.,Karunarathna, A.,Li, C. G.,Phukhamsakda, C.,Senanayake, I. C.,Wanasinghe, D. N.,Mapook, A.,Karunarathna, A.,Camporesi, E.,Camporesi, E.,Bulgakov, T. S.. 2018

[7]A candidate RxLR effector from Plasmopara viticola can elicit immune responses in Nicotiana benthamiana. Li, Xinlong,Liu, Yunxiao,Zhang, Yali,Lu, Jiang,Xiang, Jiang,Lu, Jiang,Yin, Ling,Qu, Junjie,Lu, Jiang. 2017

[8]Characteristics of New Triploid Seedless Table Grape Cultivar 'Champion Seedless' Released in China. Zhao, S. J.,Zhang, X. Z.,Guo, Z. J.,Ma, A. H.. 2009

[9]Species of Botryosphaeriaceae involved in grapevine dieback in China. Zhang, Wei,Yao, Sheng-Wei,Dissanayake, Asha J.,Li, Xing-Hong,Yan, Ji-Ye,Xie, Yue,Zhang, Guo-Zhen,Peng, You-Liang,Wang, Yong,Liu, Jian-Kui,Hyde, Kevin D.,Seem, Robert C.,Wang, Zhong-Yue,Bai, Xian-Jin. 2013

[10]Breeding of disease-resistant seedless grapes using Chinese wild Vitis spp. I. In vitro embryo rescue and plant development. Tian, Lili,Wang, Yuejin,Tang, Dongmei,Tian, Lili,Wang, Yuejin,Tang, Dongmei,Tian, Lili,Wang, Yuejin,Tang, Dongmei,Niu, Liang. 2008

[11]Endophytic Bacterium Pseudomonas fluorescens RG11 May Transform Tryptophan to Melatonin and Promote Endogenous Melatonin Levels in the Roots of Four Grape Cultivars. Ma, Yaner,Jiao, Jian,Fan, Xiucai,Sun, Haisheng,Zhang, Ying,Jiang, Jianfu,Liu, Chonghuai,Jiao, Jian. 2017

[12]Improvement of Agrobacterium-mediated transformation efficiency and transgenic plant regeneration of Vitis vinifera L. by optimizing selection regimes and utilizing cryopreserved cell suspensions. Li, P,Hanania, U,Sahar, N,Mawassi, M,Gafny, R,Sela, I,Tanne, E,Perl, A.

[13]Attractiveness of host volatiles combined with background visual cues to the tea leafhopper, Empoasca vitis. Cai, Xiao-Ming,Xu, Xiu-Xiu,Bian, Lei,Luo, Zong-Xiu,Xin, Zhao-Jun,Chen, Zong-Mao,Cai, Xiao-Ming.

[14]Expression and tissue and subcellular localization of anthocyanidin synthase (ANS) in grapevine. Wang, Huiling,Wang, Wei,Li, Hui,Zhang, Ping,Zhan, Jicheng,Huang, Weidong,Wang, Wei.

[15]Genome-wide assessment of population structure, linkage disequilibrium and resistant QTLs in Chinese wild grapevine. Zhang, Ying,Fan, Xiucai,Jiang, Jianfu,Sun, Haisheng,Liu, Chonghuai,Feng, Li,Zheng, Xian-bo.

[16]Comprehensive diversity analysis of viroids infecting grapevine in China and Japan. Jiang, Dongmei,Zhang, Zhixiang,Guo, Rui,Li, Shifang,Jiang, Dongmei,Xie, Lianhui,Wu, Zujian,Sano, Teruo,Tsuji, Masaharu,Araki, Hiroyuki,Sagawa, Kyota,Purushothama, Charith Raj Adkar,Wang, Hongqing.

[17]Effect of root restriction on nitrogen levels and glutamine synthetase activity in 'Kyoho' grapevines. Yu, Xiuming,Wang, Bo,Zhang, Caixi,Xu, Wenping,He, Jianjun,Wang, Shiping,Zhu, Lina. 2012

[18]Molecular characterization of Chinese Hop stunt viroid isolates reveals a new phylogenetic group and possible cross transmission between grapevine and stone fruits. Guo, Rui,Mu, Lingxiao,Yang, Yuanai,Li, Shifang,Zhou, Ying,Wang, Hongqing. 2012

[19]VvMJE1 of the grapevine (Vitis vinifera) VvMES methylesterase family encodes for methyl jasmonate esterase and has a role in stress response. Zhao, Nan,Chen, Xinlu,Chen, Feng,Lin, Hong,Lan, Suque,Lan, Suque,Jia, Qidong,Chen, Feng,Guo, Hong.

[20]The phenotype of grape leaves caused by acetochlor or fluoroglycofen, and effects of latter herbicide on grape leaves. Tan, Wei,Liang, Ting,Du, Yuanpeng,Zhai, Heng,Tan, Wei,Li, Qingliang.

作者其他论文 更多>>

微信小程序