Effects of the Transforming Growth Factor Beta Signaling Pathway on the Differentiation of Chicken Embryonic Stem Cells into Male Germ Cells

文献类型： 外文期刊

第一作者： Zhang, Yani

作者： Zhang, Yani;Wang, Yingjie;Zuo, Qisheng;Li, Dong;Zhang, Wenhui;Lian, Chao;Tang, Beibei;Xiao, Tianrong;Wang, Man;Li, Bichun;Zhang, Yani;Wang, Yingjie;Zuo, Qisheng;Li, Dong;Zhang, Wenhui;Lian, Chao;Tang, Beibei;Xiao, Tianrong;Wang, Man;Li, Bichun;Wang, Kehua

作者机构：

期刊名称：Cellular Reprogramming （ 影响因子：1.987； 五年影响因子：1.887 ）

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收录情况： SCI

摘要： The objectives of the present study were to screen for key gene and signaling pathways involved in the production of male germ cells in poultry and to investigate the effects of the transforming growth factor beta (TGF-beta) signaling pathway on the differentiation of chicken embryonic stem cells (ESCs) into male germ cells. The ESCs, primordial germ cells, and spermatogonial stem cells (SSCs) were sorted using flow cytometry for RNA sequencing (RNA-seq) technology. Male chicken ESCs were induced using 40ng/mL of bone morpho-genetic protein 4 (BMP4). The effects of the TGF-beta signaling pathway on the production of chicken SSCs were confirmed by morphology, quantitative real-time polymerase chain reaction, and immunocytochemistry. One hundred seventy-three key genes relevant to development, differentiation, and metabolism and 20 signaling pathways involved in cell reproduction, differentiation, and signal transduction were identified by RNA-seq. The germ cells formed agglomerates and increased innumber 14 days after induction by BMP4. During the induction process, the ESCs, Nanog, and Sox2 marker gene expression levels decreased, whereas expression of the germ cell-specific genes Stra8, Dazl, integrin-u.6, and c-kit increased. The results indicated that the TGF-beta signaling pathway participated in the differentiation of chicken ESCs into male germ cells.

分类号： Q7

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