Physiology and pathogenicity of cpdB deleted mutant of avian pathogenic Escherichia coli

文献类型： 外文期刊

第一作者： Liu, Huifang

作者： Liu, Huifang;Chen, Liping;Si, Wei;Wang, Chunlai;Liu, Siguo;Liu, Huifang;Zhu, Fangna;Liu, Huifang;Li, Guangxing

作者机构：

关键词： Avian pathogenic Escherichia coli (APEC);cpdB gene;Deletion;Physiology;Pathogenicity

期刊名称：RESEARCH IN VETERINARY SCIENCE （ 影响因子：2.534； 五年影响因子：2.382 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Avian colibacillosis is one of the most common infectious diseases caused partially or entirely by avian pathogenic Escherichia coli (APEC) in birds. In addition to spontaneous infection, APEC can also cause secondary infections that result in greater severity of illness and greater losses to the poultry industry. In order to assess the role of 2', 3'-cyclic phosphodiesterase (cpdB) in APEC on disease physiology and pathogenicity, an avian pathogenic Escherichia coli-34 (APEC-34) cpdB mutant was obtained using the Red system. The cpdB mutant grew at a slower rate than the natural strain APEC-34. Scanning electron microscopy (SEM) indicated that the bacteria of the cpdB mutant were significantly longer than the bacteria observed in the natural strain (P < 0.01), and that the width of the cpdB mutant was significantly smaller than its natural counterpart (P < 0.01). In order to evaluate the role of cpdB in APEC in the colonization of internal organs (lung, liver and spleen) in poultry, seven-day-old SPF chicks were infected with 109 CFU/chick of the cpdB mutant or the natural strain. No colonizations of cpdB mutants were observed in the internal organs 10 days following the infection, though numerous natural strains were observed at 20 days following infection. Additionally, the relative expression of division protein ftsZ, outer membrane protein A ompA, ferric uptake regulator fur and tryptophanase tnaA genes in the mutant strain were all significantly lower than in the natural strain (P < 0.05 or P < 0.01). These results suggested that cpdB is involved in the long-term colonization of APEC in the internal organs of the test subjects. The deletion of the cpdB gene also significantly affected the APEC growth and morphology. (C) 2016 Published by Elsevier Ltd.

分类号： S85

  • 相关文献

[1]Decrease of colonization in the chicks' cecum and internal organs of Salmonella enterica serovar Pullorum by deletion of cpdB by Red system. Liu, Huifang,Chen, Liping,Wang, Xiumei,Si, Wei,Wang, Huanan,Wang, Chunlai,Liu, Siguo,Liu, Huifang,Liu, Huifang,Li, Guangxing.

[2]A polyphasic approach for studying Colletotrichum. Cai, L.,Hyde, K. D.,Hyde, K. D.,Phoulivong, S.,Prihastuti, H.,Taylor, P. W. J.,Weir, B. S.,McKenzie, E. H. C.,Johnston, P. R.,Waller, J. M.,Abang, M. M.,Zhang, J. Z.,Yang, Y. L.,Liu, Z. Y.,Shivas, R. G.. 2009

[3]Insertions/Deletions-Associated Nucleotide Polymorphism in Arabidopsis thaliana. Guo, Changjiang,Wang, Long,Yang, Sihai,Tian, Dacheng,Du, Jianchang,Mauricio, Rodney,Gu, Tingting,Gu, Tingting. 2016

[4]Molecular characterization of a novel defective DNA isolated from tobacco tissues infected with tobacco leaf curl virus. Zhou, XP,Xie, Y,Zhang, ZK,Qi, YJ,Wu, JJ. 2001

[5]Mutations in the genome of the highly pathogenic porcine reproductive and respiratory syndrome virus potentially related to attenuation. Leng, Xue,Li, Zhenguang,Wang, Fengxue,Wang, Wei,Zhang, Xiuhua,Wu, Hua,Xia, Mingqi,Li, Xiyu,Wu, Hua.

[6]The characterization of GmTIP, a root-specific gene from soybean, and the expression analysis of its promoter. Chen, Li,Jiang, Bingjun,Wu, Cunxiang,Sun, Shi,Hou, Wensheng,Han, Tianfu.

[7]The ecdysteroid UDP-glucosyltransferase gene promoter from Autographa californica multicapsid nucleopolyhedrovirus. Shen, XJ,Yi, YZ,Tang, SM,Zhang, ZF,Li, YR,He, JL.

[8]ORF3a deletion in field strains of porcine-transmissible gastroenteritis virus in China: A hint of association with porcine respiratory coronavirus. Zhang, X.,Zhu, Y.,Zhu, X.,Chen, J.,Shi, H.,Shi, D.,Dong, H.,Feng, L.,Dong, H..

[9]Genome Editing in Cotton with the CRISPR/Cas9 System. Wei Gao,Lu Long,Song, Chunpeng,Xinquan Tian,Ji Liu,Prashant K. Singh,Jose R. Botella,Chunpeng Song. 2017

[10]GmPRP2 promoter drives root-preferential expression in transgenic Arabidopsis and soybean hairy roots. Chen, Li,Jiang, Bingjun,Wu, Cunxiang,Sun, Shi,Hou, Wensheng,Han, Tianfu. 2014

[11]Mapping the morphogenetic potential of antler fields through deleting and transplanting subregions of antlerogenic periosteum in sika deer (Cervus nippon). McMahon, Chris,Li, Chunyi,Gao, Zhiguang,Yang, Fuhe,Gao, Zhiguang,Gao, Zhiguang,Yang, Fuhe,Li, Chunyi. 2012

[12]The Fate of Arabidopsis thaliana Homeologous CNSs and Their Motifs in the Paleohexaploid Brassica rapa. Subramaniam, Sabarinath,Freeling, Michael,Wang, Xiaowu,Pires, J. Chris. 2013

[13]Molecular characterization and functional analysis of barley semi-dwarf mutant Riso no. 9265. Jia, Qiaojun,Zhang, Guoping,Jia, Qiaojun,Shang, Yi,Zhu, Jinghuan,Hua, Wei,Wang, Junmei,Yang, Jianming,Li, Chengdao. 2015

[14]Effects of spaceflight on polysaccharides of Saccharomyces cerevisiae cell wall. Liu, Hong-Zhi,Wang, Qiang,Liu, Xiao-Yong,Tan, Sze-Sze.

[15]Influence of minor displacements in loops of the porcine parvovirus VP2 capsid on virus-like particles assembly and the induction of antibody responses. Pan, Qunxing,He, Kongwang,Wang, Yongshan,Wang, Xiaoli,Ouyang, Wei.

[16]Effects of salt stress on rice growth, development characteristics, and the regulating ways: A review. Hussain, Sajid,Zhang Jun-hua,Zhong Chu,Zhu Lian-feng,Cao Xiao-chuang,Yu Sheng-miao,James, Allen Bohr,Hu Ji-jie,Jin Qian-yu. 2017

[17]Effects of waterlogging at different growth stages on physiological characteristics and seed yield of winter rape (Brassica napus L). Zhou, WJ,Lin, XQ. 1995

[18]Oxidative Stress and Antioxidant Defenses after Long-term Fasting in Blood of Chinese Sturgeon (Acipenser sinensis). Feng, Guangpeng,Huang, Xiaorong,Zhuang, Ping,Shi, Xiaotao. 2011

[19]Effect of Feeding Rate on Growth Performance, Feed Utilization, and Blood-Chemistry Indicators of Nutritional Status in Juvenile Gift Strain Tilapia (Oreochromis Niloticus L.). Didlyn, Kpundeh Mathew,Pao, Xu,Didlyn, Kpundeh Mathew,Pao, Xu,Jun, Qiang,Hong, Yang,Jie, He,Didlyn, Kpundeh Mathew. 2015

[20]Effects of Temperature on Oxidative Stress Biomarkers in Juvenile Chinese Sturgeon (Acipenser Sinensis) under Laboratory Conditions. Feng, Guangpeng,Zhuang, Ping,Zhang, Longzhen,Duan, Ming,Liu, Jianyi. 2012

作者其他论文 更多>>

微信小程序