MicroRNA expression profiling of primary sheep testicular cells in response to bluetongue virus infection

文献类型： 外文期刊

第一作者： Du, Junzheng

作者： Du, Junzheng;Gao, Shandian;Tian, Zhancheng;Xing, Shanshan;Huang, Dexuan;Zhang, Guorui;Zheng, Yadong;Liu, Guangyuan;Luo, Jianxun;Chang, Huiyun;Yin, Hong;Yin, Hong

作者机构：

关键词： Primary sheep testicular cells;Bluetongue virus;RNA sequencing;microRNAs

期刊名称：INFECTION GENETICS AND EVOLUTION （ 影响因子：3.342； 五年影响因子：3.188 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Bluetongue virus (BTV) is a member of the genus Orbivirus within the family Reoviridae and causes a non-contagious, insect-transmitted disease in domestic and wild ruminants, mainly in sheep and occasionally in cattle and some species of deer. Virus infection can trigger the changes of the cellular microRNA (miRNA) expression profile, which play important post-transcriptional regulatory roles in gene expression and can greatly influence viral replication and pathogenesis. Here, we employed deep sequencing technology to determine which cellular miRNAs were differentially expressed in primary sheep testicular (ST) cells infected with BTV. A total of 25 known miRNAs and 240 novel miRNA candidates that were differentially expressed in BTV-infected and uninfected ST cells were identified, and 251 and 8428 predicted target genes were annotated, respectively. Nine differentially expressed miRNAs and their mRNA targets were validated by quantitative reverse transcription-polymerase chain reaction. Targets prediction and functional analysis of these regulated miRNAs revealed significant enrichment for several signaling pathways including MAPK, PI3K-Akt, endocytosis, Hippo, NF-kB, viral carcinogenesis, FoxO, and JAK-STAT signaling pathways. This study provides a valuable basis for further investigation on the roles of miRNAs in BTV replication and pathogenesis. (C) 2017 Elsevier B.V. All rights reserved.

分类号： R1

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