Reduced ascorbate and reduced glutathione improve embryogenesis in broccoli microspore culture

文献类型： 外文期刊

第一作者： Zeng, A.

作者： Zeng, A.;Song, L.;Yan, J.;Cui, Y.

作者机构：

关键词： Broccoli (Brassica oleracea L. Var. italica);Microspore culture;Embryogenesis;Cell death;Reduced ascorbate;Reduced glutathione

期刊名称：SOUTH AFRICAN JOURNAL OF BOTANY （ 影响因子：2.315； 五年影响因子：2.71 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Isolated microspore culture (IMC) is an effective alternative technique for the production of doubled haploid (DH) homozygous lines in plant breeding. However, death of most of the cultured cells during culture is a critical obstacle that affects embryo production in the practical application of IMC technology. In this present paper, we evaluated the effects of two antioxidants of both reduced ascorbate (ASC) and reduced glutathione (GSH) onmicrospore viability and the induction of embryogenesis in the IMC of broccoli (Brassica oleracea L. var. italica). The results showed that cell death occurred soon after stress treatment (at 32.5 degrees C for 24 h), and the number of dead cells reached about 80% after 2 d. The addition of 10 mg l(-1) ASC and 20 mg l(-1) GSH significantly decreased microspore mortality, and had a strong effect on the number of embryos produced. In the genotype 'B415', embryo yields increased approximately 1.2-fold and 2.5-fold after the addition of the 10 mg l(-1) ASC and 20 mg l(-1) GSH, respectively. Moreover, the addition of ASC and GSH also influenced positively the rate of plant regeneration, which respectively increased by 4.2% and 9.7% for 'B415'. Taken together, our results show that the induction of embryogenesis in broccoli is strongly affected by the use of ASC or GSH. The effects on embryogenesis induction could confirm the importance of the redox environmental regulation in IMC, specifically at the early phase of IMC. The obtained knowledge may accelerate and guide the application of microspore-derived DH plants as a breeding tool in broccoli. (C) 2017 SAAB. Published by Elsevier B.V. All rights reserved.

分类号： Q94

  • 相关文献

[1]High frequency spontaneous production of doubled haploid plants in microspore cultures of Brassica rapa ssp chinensis. Gu, HH,Zhou, WJ,Hagberg, P. 2003

[2]Effects of pH, MES, arabinogalactan-proteins on microspore cultures in white cabbage. Yuan, Su-xia,Su, Yan-bin,Liu, Yu-mei,Fang, Zhi-yuan,Yang, Li-mei,Zhuang, Mu,Zhang, Yang-yong,Sun, Pei-tian.

[3]Microspore embryogenesis and plant regeneration in Brussels sprouts (Brassica oleracea L. var. gemmifera). Zeng, Aisong,Yan, Yuanyuan,Song, Lixiao,Hou, Xilin,Li, Ying,Zeng, Aisong,Yan, Yuanyuan,Yan, Jiyong,Song, Lixiao,Gao, Bing,Li, Jianqi.

[4]Colorimetric Sensing of Cysteine in serum by the competitive adsorption toward AuNPs between Cysteine and ATP. Zhao, Lingzhi,Miao, Yanqing,Liu, Chunye,Zhao, Liu,Zhao, Lingzhi. 2015

[5]Reduced Glutathione Mediates Phone-Ultrastrcture, Kinome and Transportome in Chromium-Induced Brassica napus L.. Gill, Rafaqat A.,Ali, Basharat,Yang, Su,Islam, Faisal,Gill, Muhammad Bilal,Mwamba, Theodore M.,Ali, Skhawat,Zhou, Weijun,Gill, Rafaqat A.,Ali, Basharat,Yang, Su,Islam, Faisal,Gill, Muhammad Bilal,Mwamba, Theodore M.,Ali, Skhawat,Zhou, Weijun,Gill, Rafaqat A.,Mao, Bizeng,Ali, Basharat,Tong, Chaobo,Liu, Shengyi. 2017

[6]Impact of genotype, plant growth regulators and activated charcoal on embryogenesis induction in microspore culture of pepper (Capsicum annuum L.). Cheng, Yan,Ma, Rong-li,Jiao, Yan-sheng,Qiao, Ning. 2013

[7]Efficient doubled haploid production in microspore culture of loose-curd cauliflower (Brassica oleracea var. botrytis). Gu, Honghui,Zhao, Zhengqing,Sheng, Xiaoguang,Yu, Huifang,Wang, Jiansheng. 2014

[8]Microspore embryogenesis and production of haploid and doubled haploid plants in carrot (Daucus carota L.). Li, Jin-Rong,Zhuang, Fei-Yun,Ou, Cheng-Gang,Hu, Hong,Zhao, Zhi-Wei,Mao, Ji-Hua.

[9]Initiation and development of microspore embryogenesis and plant regeneration of Brassica nigra. Gu, Honghui,Sheng, Xiaoguang,Zhao, Zhenqing,Yu, Huifang,Wang, Jiansheng.

[10]Studying the Mechanism of Plasmopara viticola RxLR Effectors on Suppressing Plant Immunity. Li, Xinlong,Wu, Jiao,Zhang, Yali,Lu, Jiang,Yin, Ling. 2016

[11]Somatic embryogenesis receptor-like kinase 5 in the ecotype Landsberg erecta of Arabidopsis is a functional RD LRR-RLK in regulating brassinosteroid signaling and cell death control. Wu, Wangze,Wu, Yujun,Gao, Yang,Li, Meizhen,Yin, Hongju,Lv, Minghui,Zhao, Jianxin,Li, Jia,He, Kai,Wu, Wangze. 2015

[12]Effect of nerve injury on the number of dorsal root ganglion neurons and autotomy behavior in adult Bax-deficient mice. Lyu, Chuang,Lyu, Chuang,Lyu, Gong-Wei,Martinez, Aurora,Shi, Tie-Jun Sten. 2017

[13]Semi-dominant mutations in the CC-NB-LRR-type R gene, NLS1, lead to constitutive activation of defense responses in rice. Tang, Jiuyou,Wang, Yiqin,Liu, Linchuan,Xu, Bo,Li, Feng,Fang, Jun,Chu, Chengcai,Tang, Jiuyou,Wang, Yiqin,Liu, Linchuan,Xu, Bo,Li, Feng,Fang, Jun,Chu, Chengcai,Tang, Jiuyou,Liu, Linchuan,Xu, Bo,Li, Feng,Zhu, Xudong.

[14]The roles of ascorbic acid and glutathione in symptom alleviation to SA-deficient plants infected with RNA viruses. Xu, Fei,Shang, Jing,Xu, Mo-Yun,Wang, Jian-Hui,Xi, De-Hui,Zhu, Feng,Yuan, Shu,Yang, Hui,Jia, Shu-Dan,Zhang, Zhong-Wei,Lin, Hong-Hui,Wang, Jian-Hui.

[15]Arabidopsis FHY3 and FAR1 Regulate Light-Induced myo-Inositol Biosynthesis and Oxidative Stress Responses by Transcriptional Activation of MIPS1. Ma, Lin,Tian, Tian,Li, Gang,Lin, Rongcheng,Deng, Xing-Wang,Wang, Haiyang. 2016

[16]The effects of ultrasound and arsenic trioxide on neurogliocytoma cells and secondary activation of macrophages. Xu, Yonggang,Liu, Xiaoqian,Wang, Zhi,Yue, Wu,Wang, Jie,Zhang, Yafang,Ma, Jing. 2009

[17]Thymol Mitigates Cadmium Stress by Regulating Glutathione Levels and Reactive Oxygen Species Homeostasis in Tobacco Seedlings. Ye, Xiefeng,Ling, Tianxiao,Xue, Yanfeng,Xu, Cunfa,Zhou, Wei,Hu, Liangbin,Chen, Jian,Shi, Zhiqi,Chen, Jian,Shi, Zhiqi.

[18]Cinnamaldehyde Ameliorates Cadmium-Inhibited Root Elongation in Tobacco Seedlings via Decreasing Endogenous Hydrogen Sulfide Production. Ye, Xie-Feng,Ling, Tianxiao,Yu, Xiao-Na,Xue, Yanfeng,Wang, Yong,Cheng, Changxin,Feng, Guosheng,Hu, Liangbin,Shi, Zhiqi,Chen, Jian,Shi, Zhiqi,Chen, Jian.

[19]The phytotoxic effects of selenium-mercury interactions on root growth in Brassica rapa (LvLing). Bian, Zhi-Wei,Li, Hui,Liu, Dan-Dan,Yang, Li-Fei,Zhu, Yue-Lin,Zhu, Wen-Li,Liu, Wei,Ying, Zheng-Zheng,Chen, Jian.

[20]Response of vacuolar processing enzyme in Malus hupehensis and MhVPE gamma-overexpressing Arabidopsis to high temperature stress. Su, Qian,Men, Xiu-Jin,Zhang, Wei-Wei,Fan, Shu-Lei,Yan, Li-Juan,Yang, Hong-Qiang,Ran, Kun.

作者其他论文 更多>>

微信小程序