Utility of Thermostable Xylanases of Mycothermus thermophilus in Generating Prebiotic Xylooligosaccharides

文献类型: 外文期刊

第一作者: Ma, Rui

作者: Ma, Rui;Bai, Yingguo;Huang, Huoqing;Luo, Huiying;Yao, Bin;Ma, Rui;Fan, Yunliu;Ma, Rui;Chen, Sanfeng;Cai, Lei

作者机构:

关键词: xylanase;Mycothermus thermophilus;xylooligosaccharides;prebiotic;probiotic Lactobacillus

期刊名称:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ( 影响因子:5.279; 五年影响因子:5.269 )

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收录情况: SCI

摘要: Xylooligosaccharides as emerging prebiotics are able to promote the growth of probiotic bacteria. In the present study, four neutral, thermostable xylanases (MtXyn11A, MtXyn11At, MtXyn11B, and MtXyn11C) from compost fungus Mycothermus thermophilus CGMCC3.18119 were overexpressed in Pichia pastoris GS115 and used to produce xylooligosaccharides from beechwood xylan. The enzymes showed similar enzymatic properties (maximal activities at pH 6.0-6.5 and 65 degrees C) but varied in catalytic efficiency and cleaving actions. MtXyn11A, MtXyn11At, and MtXyn11C mainly produced xylobiose (59-62%), xylose (16-20%), and xylotriose (16-19%), while MtXyn11B released xylobiose (51%), xylotriose (32%), and xylose (12%) as the main products. When using the xylan hydrolysates of different xylanases as the carbon source, four probiotic Lactobacillus strains Lactobacillus brevis 1.2028, Lactobacillus rhamnosus GG, Lactobacillus casei BL23, and Lactobacillus plantarum WCSF1 were confirmed to use the xylooligosaccharides efficiently (83.8-98.2%), with L. brevis 1.2028 as the greatest.

分类号: R15`S

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