LetR is a TetR family transcription factor from Lysobacter controlling antifungal antibiotic biosynthesis

文献类型: 外文期刊

第一作者: Wang, Ping

作者: Wang, Ping;Chen, Hongfu;Qian, Guoliang;Liu, Fengquan;Liu, Fengquan

作者机构:

关键词: Lysobacter;HSAF;TetR;Regulation;Antibiotics

期刊名称:APPLIED MICROBIOLOGY AND BIOTECHNOLOGY ( 影响因子:4.813; 五年影响因子:4.697 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Heat-stable antifungal factor (HSAF) is a newly identified and broad-spectrum antifungal antibiotic from Lysobacter enzymogenes, a ubiquitous environmental proteobacterium. Yet, the regulatory mechanism for HSAF biosynthesis in L. enzymogenes remains poorly understood. Here, we report the identification of a TetR-family protein Le1552 (LetR) from L. enzymogenes strain OH11 that is involved in transcriptional repression of HSAF production. Bacterial one-hybrid and gel mobility shift assays show that LetR directly binds to PHSAF (the promoter region of the HSAF biosynthesis operon). A DNA truncation assay further reveals a core region in PHSAF that is responsible for LetR binding. In-frame deletion of letR in wild-type OH11 is found to significantly increase HSAF levels and key biosynthetic gene transcription, while overexpression of letR in the wild-type background remarkably reduces HSAF levels as well as related gene expression instead. Together, we have identified not only a new regulator for the HSAF biosynthesis but also constructed a higher HSAF-producing deletion strain (Delta letR) of L. enzymogenes, which shall be of great value in promoting HSAF production for pharmaceutical and biological control purposes.

分类号: Q939.9`Q81

  • 相关文献

[1]Lysobacter PilR, the Regulator of Type IV Pilus Synthesis, Controls Antifungal Antibiotic Production via a Cyclic di-GMP Pathway. Chen, Yuan,Xia, Jing,Su, Zhenhe,Xu, Gaoge,Qian, Guoliang,Liu, Fengquan,Liu, Fengquan,Gomelsky, Mark.

[2]Lsp family proteins regulate antibiotic biosynthesis in Lysobacter enzymogenes OH11. Wang, Ruping,Xu, Huiyong,Zhao, Yangyang,Liu, Fengquan,Wang, Ruping,Zhang, Juan,Qian, Guoliang,Liu, Fengquan,Yuen, Gary Y.. 2017

[3]Heat-Stable Antifungal Factor (HSAF) Biosynthesis in Lysobacter enzymogenes Is Controlled by the Interplay of Two Transcription Factors and a Diffusible Molecule. Su, Zhenhe,Liu, Fengquan,Su, Zhenhe,Han, Sen,Qian, Guoliang,Liu, Fengquan,Su, Zhenhe,Han, Sen,Qian, Guoliang,Liu, Fengquan,Fu, Zheng Qing. 2018

[4]Yield Improvement of the Anti-MRSA Antibiotics WAP-8294A by CRISPR/dCas9 Combined with Refactoring Self-Protection Genes in Lysobacter enzymogenes OH11. Yu, Lingjun,Liu, Fengquan,Yu, Lingjun,Su, Wei,Du, Liangcheng,Fey, Paul D.. 2018

[5]Transcriptional and Antagonistic Responses of Biocontrol Strain Lysobacter enzymogenes OH11 to the Plant Pathogenic Oomycete Pythium aphanidermatum. Zhao, Yangyang,Liu, Fengquan,Qian, Guoliang,Chen, Yuan,Du, Liangcheng. 2017

[6]LesR is a novel upstream regulator that controls downstream Clp expression to modulate antibiotic HSAF biosynthesis and cell aggregation in Lysobacter enzymogenes OH11. Xu, Huiyong,Zhao, Yangyang,Liu, Fengquan,Wang, Ruping,Qian, Guoliang,Fu, Zheng Qing. 2017

[7]Transcriptomic analysis reveals new regulatory roles of Clp signaling in secondary metabolite biosynthesis and surface motility in Lysobacter enzymogenes OH11. Wang, Yansheng,Zhao, Yuxin,Zhang, Juan,Zhao, Yangyang,Shen, Yan,Su, Zhenhe,Xu, Gaoge,Qian, Guoliang,Liu, Fengquan,Liu, Fengquan,Du, Liangcheng,Huffman, Justin M.,Venturi, Vittorio. 2014

[8]Type IV pilus biogenesis genes and their roles in biofilm formation in the biological control agent Lysobacter enzymogenes OH11. Xia, Jing,Chen, Jiaojiao,Chen, Yuan,Qian, Guoliang,Liu, Fengquan,Liu, Fengquan. 2018

[9]Production of Antifungal p-Aminobenzoic Acid in Lysobacter antibioticus OH13. Laborda, Pedro,Zhao, Yangyang,Ling, Jun,Hou, Rongxian,Liu, Fengquan. 2018

[10]ChpA Controls Twitching Motility and Broadly Affects Gene Expression in the Biological Control Agent Lysobacter enzymogenes. Zhou, Mimi,Shen, Danyu,Xu, Gaoge,Qian, Guoliang,Liu, Fengquan.

[11]Dietary risk ranking for residual antibiotics in cultured aquatic products around Tai Lake, China. Song, Chao,Zhang, Cong,Qiu, Liping,Fan, Limin,Wu, Wei,Meng, Shunlong,Hu, Gengdong,Chen, Jiazhang,Song, Chao,Zhang, Cong,Qiu, Liping,Fan, Limin,Wu, Wei,Meng, Shunlong,Hu, Gengdong,Chen, Jiazhang,Song, Chao,Li, Le,Zhang, Cong,Qiu, Liping,Fan, Limin,Wu, Wei,Meng, Shunlong,Hu, Gengdong,Chen, Jiazhang,Li, Le,Liu, Ying,Mao, Aimin. 2017

[12]A simple and economic method for simultaneous determination of 11 antibiotics in manure by solid-phase extraction and high-performance liquid chromatography. Feng, Yao,Zhang, Wenjuan,Liu, Yuanwang,Li, Zhaojun,Hu, Haiyan,Wei, Chaojun,Zhang, Wenjuan,Xue, Jianming. 2016

[13]Interactive Effects of Tetracyclines and Copper on Plant Growth and Nutrient Uptake by Eichhornia crassipes. Lu, Xin,Gao, Yan,Luo, Jia,Yan, Shaohua,Wang, Tong,Liu, Lizhu,Zhang, Zhenhua,Lu, Xin,Gao, Yan,Luo, Jia,Yan, Shaohua,Wang, Tong,Liu, Lizhu,Zhang, Zhenhua. 2016

[14]Identification of the streptothricin and tunicamycin biosynthetic gene clusters by genome mining in Streptomyces sp strain fd1-xmd. Yu, Yucong,Tang, Biao,Dai, Ruixue,Zhang, Bo,Chen, Liqiang,Zhao, Guoping,Ding, Xiaoming,Tang, Biao,Yang, Hua,Zhao, Guoping. 2018

[15]Temporal-spatial variation and partitioning prediction of antibiotics in surface water and sediments from the intertidal zones of the Yellow River Delta, China. Zhao, Shengnan,Liu, Xinhui,Liang, Baocui,Cui, Baoshan,Bai, Junhong,Cheng, Dengmiao,Liu, Guannan.

[16]Interspecific differences in growth response and tolerance to the antibiotic sulfadiazine in ten clonal wetland plants in South China. Chen, Jinfeng,Sun, Yingbo,Huang, Lili,Zhang, Peixia,Zou, Chunping,Yu, Bo,Zhu, Genfa,Zhao, Chaoyi,Xu, Huilian.

[17]Occurrence, fate, and ecotoxicity of antibiotics in agro-ecosystems. A review. Liu, Wenke,Du, Lianfeng,Liu, Wenke.

[18]Changes in antimicrobial resistance among Salmonella enterica subspecies enterica serovar Pullorum isolates in China from 1962 to 2007. Pan, Zhiming,Wang, Xiaoquan,Zhang, Xiaoming,Geng, Shizhong,Chen, Xiang,Pan, Weijuan,Cong, Qiuxia,Jiao, Xinan,Liu, Xiufan,Liu, Xuexian.

[19]Comparative proteomic analysis reveals intracellular targets for bacillomycin L to induce Rhizoctonia solani Kuhn hyphal cell death. Zhang, Bao,Qin, Yuxuan,Han, Yuzhu,Li, Pinglan,Qin, Yuxuan,Li, Pinglan,Zhang, Bao,Dong, Chunjuan,Shang, Qingmao.

[20]Antibiotic-induced male-sterility in oilseed flax. Dang, ZH,Zhang, JP,She, X. 2000

作者其他论文 更多>>