FIP-sch2, a new fungal immunomodulatory protein from Stachybotrys chlorohalonata, suppresses proliferation and migration in lung cancer cells

文献类型： 外文期刊

第一作者： Li, Shuying

作者： Li, Shuying;Jiang, Zhonghao;Xu, Wenyi;Xie, Yingying;Zhao, Leiming;Tang, Xuanming;Wang, Fengzhong;Xin, Fengjiao

作者机构：

关键词： Fungal immunomodulatory protein;Proliferation;Apoptosis;Migration

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Fungal immunomodulatory protein (FIP)-sch2, an immunomodulatory protein identified in the ascomycete Stachybotrys chlorohalonata by a sequence similarity search, is a novel member of the FIP family. FIP-sch2 shares high sequence identity, structure, and evolutionary conservation with previously reported FIPs. It was satisfactorily expressed in Escherichia coli with a glutathione S-transferase (GST) tag and purified by GST-affinity magnetic beads. To characterize the direct antitumor effects, human lung adenocarcinoma A549 cells were treated with different concentrations of recombinant FIP (rFIP)-sch2 in vitro, and the results showed that rFIP-sch2 could reduce cell viability dose-dependently with a half-maximal inhibitory concentration (IC50) of 9.48 mu g/mL. Furthermore, rFIP-sch2 at 8 mu g/mL could significantly induce apoptosis and interrupt migration in A549 cells. Notably, the antitumor effect of rFIP-sch2 was equivalent to that of rLZ-8 but was obviously increased compared to rFIP-fve. In addition, the exploration of the antitumor mechanism suggested that rFIP-sch2 induced lung cancer cell death by activating apoptosis and inhibiting migration. Our results indicated that rFIP-sch2 was a promising candidate for use in future cancer therapy.

分类号： Q939.9`Q81

  • 相关文献

[1]Characterization of a new fungal immunomodulatory protein, FIP-dsq2 from Dichomitus squalens. Li, Shuying,Jiang, Zhonghao,Sun, Lichao,Liu, Xin,Huang, Ying,Wang, Fengzhong,Xin, Fengjiao.

[2]Up-regulation of miR-182-5p predicts poor prognosis in patients with lung cancer and associates with tumor cell growth and migration. Shi, Yuanyuan,Liu, Hao,Du, Xiang,Liu, Xuedong,Liu, Jing. 2017

[3]Epidermal Growth Factor Promotes Proliferation and Migration of Follicular Outer Root Sheath Cells via Wnt/beta-Catenin Signaling. Zhang, Haihua,Wang, Shiyong,Zhang, Tietao,Yang, Fuhe,Li, Guangyu,Nan, Weixiao,Si, Huazhe. 2016

[4]Knockdown of HIF-1 alpha inhibits the proliferation and migration of outer root sheath cells exposed to hypoxia in vitro: An involvement of Shh pathway. Zhang, Haihua,Song, Xingchao,Wang, Shiyong,Si, Huazhe,Li, Guangyu,Nan, Weixiao. 2017

[5]In vitro Study of Nucleostemin as a Potential Therapeutic Target in Human Breast Carcinoma SKBR-3 Cells. Guo, Yu,Liao, Ya-Ping,Zhang, Ding,Xu, Li-Sha,Li, Na,Liu, Chang-Qing,Guan, Wei-Jun,Liu, Chang-Qing. 2014

[6]Over-expression of miR-146b and its regulatory role in intestinal epithelial cell viability, proliferation, and apoptosis in piglets. Tao, Xin,Liu, Shujie,Men, Xiaoming,Xu, Ziwei. 2017

[7]The injury of serotonin on intestinal epithelium cell renewal of weaned diarrhoea mice. Dong, Y.,Yang, C.,Wang, Z.,Cao, J.,Chen, Y.,Qin, Z..

[8]Antimony release from contaminated mine soils and its migration in four typical soils using lysimeter experiments. Zhao, Long,Hou, Hong,Shangguan, Yu-xian,Qin, Yusheng,Zhang, Naiming.

[9]Molecular cloning, sequence analysis, and function of the intestinal epithelial stem cell marker Bmi1 in pig intestinal epithelial cells. Li, C. -M.,Yan, H. -C.,Fu, H. -L.,Xu, G. -F.,Wang, X. -Q.. 2014

[10]The Influence of Thidiazuron on Proliferation of Acer x freemanii In Vitro. Zhao, X. Q.,Yuan, Z. H.,Yin, Y. L.,Feng, L. J.,Yin, Y. L.,Xu, R.. 2012

[11]Effect of Auxins on In Vitro Propagation of Sour Jujube Infected by Witches' Broom Phytoplasma. Sun, Q.,Zhou, G.,Sun, H.,Giang, G.. 2009

[12]Effects of linoleic acid and eicosapentaenoic acid on cell proliferation and lipid-metabolism gene expression in primary duck hepatocytes. Liu, W. M.,Lu, L. Z.,Zhang, C.,Liu, Y. L.,Zhang, J.,Tao, Z. R.,Shen, J. D.,Li, G. Q.,Wang, D. Q.,Li, J. J.,Tian, Y.,Liu, W. M.,Shi, F. X.,Liu, Y. L.,Zhang, J.. 2011

[13]Relationship of flight and reproduction in beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae), a migrant lacking the oogenesis-flight syndrome. Jiang, Xing Fu,Luo, Li Zhi,Sappington, Thomas W.. 2010

[14]Spring migration and summer dispersal of Loxostege sticticalis (Lepidoptera : Pyralidae) and other insects observed with radar in Northern China. Wu, KM,Cheng, DF,Guo, YY. 2004

[15]Variation of pre-ovipositional period in the brown planthopper, Nilaparvata lugens, collected in tropical, subtropical and temperate Asia. Wada, T.,Ito, K.,Takahashi, A.,Tang, J.. 2007

[16]The anti-inflammatory effect of the SOCC blocker SK&F 96365 on mouse lymphocytes after stimulation by Con A or PMA/ionomycin. Ye, Yanxia,Zhang, Yaxing,Lu, Xiaoyu,Huang, Xiuyan,Zeng, Xiangfeng,Zeng, Yaoying,Lu, Xiaoyu,Lai, Xinqiang. 2011

[17]Klf7 modulates the differentiation and proliferation of chicken preadipocyte. Zhang, Zhiwei,Wang, Haixia,Sun, Yingning,Li, Hui,Wang, Ning,Zhang, Zhiwei,Wang, Haixia,Sun, Yingning,Li, Hui,Wang, Ning,Zhang, Zhiwei,Wang, Haixia,Sun, Yingning,Li, Hui,Wang, Ning,Sun, Yingning. 2013

[18]Do second generation Asia corn borer (Lepidoptera: Crambidae) immigrate to corn fields from alternate habitats?. Chen, Ri-Zhao,Li, Lian-Bing,Li, Peng-Pei,Klein, Michael G.,Li, Qi-Yun,Sheng, Cheng-Fa. 2015

[19]Different genetic structures revealed resident populations of a specialist parasitoid wasp in contrast to its migratory host. Wei, Shu-Jun,Zhou, Yuan,Fan, Xu-Lei,Cao, Li-Jun,Zhou, Yuan,Xu, Zai-Fu,Hoffmann, Ary A.,Chen, Xue-Xin. 2017

[20]Migration and population structure characteristics of the small yellow croaker Larimichthys polyactis in the southern Yellow Sea. Xiong Ying,Yang Jian,Xiong Ying,Zhong Xiaming,Tang Jianhua,Yang Jian. 2016

作者其他论文 更多>>

微信小程序