Preparation of monoclonal antibody and development of an indirect competitive enzyme-linked immunosorbent assay for ornidazole detection

文献类型： 外文期刊

第一作者： Zhao, Liang

作者： Zhao, Liang;Li, Jiaying;Li, Ye;Wang, Tiangang;Rahman, Ebeydulla;Ji, Baoping;Zhou, Feng;Jin, Xiaolu;Wang, Kai;Xing, Yuan;Xing, Yuan

作者机构：

关键词： Ornidazole;Monoclonal antibodies;ELISA

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： A monoclonal antibody (mAb) and an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for ornidazole (ONZ) detection were developed. ONZ was conjugated with cationic bovine serum albumin as a hapten to generate the artificial irnmunogens and coating antigens. BALB/c mice were immunized, and mAbs were obtained. The competitive inhibition curve of ic-ELISA was y = 0.0438x(2) - 0.2101x + 0.2925, with R-2 = 0.9941. The 50% inhibition concentration, the limit of detection, and limit of quality for ONZ were 0.15, 0.01, and 0.05 mu g/kg, respectively. The cross-reactivity of the mAbs to secnidazole was 0.33%. The recoveries were from 89.18% to 101.63% and the coefficient of variation was less than 7.15% in chicken, chicken liver, and honey samples, all of which had ONZ concentrations of 0.05 and 0.1 mu g/kg. Results showed that the ic-ELISA based on mAb could be used for the rapid detection for ONZ. (C) 2017 Elsevier Ltd. All rights reserved.

分类号： TS2`TS201.2

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