Developing insect resistance with fusion gene transformation of chitinase and scorpion toxin gene in maize (Zea mays L)

文献类型: 外文期刊

第一作者: Liu, Ming

作者: Liu, Ming;Wang, Jingxue;Hao, Yaoshan;Sun, Yi

作者机构:

关键词: maize;insect-resistant gene combination;chitinase gene;scorpion insect toxin gene;transgenic plant;calibrated mortality of larvae

期刊名称:MAYDICA ( 影响因子:0.542; 五年影响因子:0.605 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Transgenic plants with introduced pest-resistant genes provide an efficient alternative insect control. A binary insect-resistant gene combination, containing an insect-specific chitinase gene (chi) and a scorpion insect toxin gene (Bmk), was introduced into a maize cultivar via pollen-mediated transformation. Thirty-eight putative transgenic plantlets with kanamycin-resistance were obtained. Transgenic statuses of plants were confirmed by Southern blot analysis. Bioassay by inoculation of Asian corn borer (Ostrinia furnacalis Guenee; ACB) larvae indicated that the degree of ACB resistance varied among the transgenic plants. The highest average calibrated mortality of larvae was approximately 67%. The genetic analysis of T1 progeny confirmed that the inheritance of introduced genes followed the Mendelian's rules.

分类号: TB

  • 相关文献

[1]Genetic transformation of the tropical forage legume Stylosanthes guianensis with a rice-chitinase gene confers resistance to Rhizoctonia foliar blight disease. Kelemu, S,Jiang, CS,Huang, GX,Segura, G. 2005

[2]A maize stress-responsive NAC transcription factor, ZmSNAC1, confers enhanced tolerance to dehydration in transgenic Arabidopsis. Lu, Min,Ying, Sheng,Zhang, Deng-Feng,Shi, Yun-Su,Song, Yan-Chun,Wang, Tian-Yu,Li, Yu. 2012

[3]Two alternative splicing variants of maize HKT1;1 confer salt tolerance in transgenic tobacco plants. Ren, Zhenjing,Kang, Dan,Fan, Kaijian,Wang, Cuiyun,Wang, Guoying,Liu, Yunjun,Liu, Yan.

[4]Transgenic maize plants expressing a fungal phytase gene. Chen, Rumei,Chen, Ping,Yang, Wenzhu,Ma, Qianli,Fan, Yunliu,Xue, Guangxing,Yao, Bin,Zhao, Zuoyu,Tarczynski, Mitchell C.,Shi, Jinrui. 2008

[5]Effects of Bt transgenic cotton lines on the cotton bollworm parasitoid Microplitis mediator in the laboratory. Liu, XX,Zhang, QW,Zhao, JZ,Li, HC,Xu, BL,Ma, XM. 2005

[6]Genetic transformation of watercress with a gene encoding for betaine-aldehyde dehydrogenase (BADH). Li, YX,Chang, FQ,Du, LQ,Guo, BH,Li, HJ,Zhang, JS,Chen, SY,Zhu, ZQ. 2000

[7]Over-expression of Oryza sativa Xrn4 confers plant resistance to virus infection. Jiang, Shanshan,Jiang, Liangliang,Jiang, Shanshan,Yang, Jian,Peng, Jiejun,Lu, Yuwen,Zheng, Hongying,Lin, Lin,Chen, Jianping,Yan, Fei,Yang, Jian,Peng, Jiejun,Lu, Yuwen,Zheng, Hongying,Lin, Lin,Chen, Jianping,Yan, Fei. 2018

[8]Expression of rabbit defensin NP-1 gene in transgenic tobacco plants and its activity against bacterial wilt. Peng, YF,Cao, GC,Ma, JS,Chen, CX,Zhang, LM,Li, WB,Sun, YR. 1998

[9]Arabidopsis LOS5 Gene Enhances Chilling and Salt Stress Tolerance in Cucumber. Liu Li-ying,Duan Liu-sheng,Zhang Jia-chang,Zhang Xiao-lan,Zhang Zhen-xian,Ren Hua-zhong,Mi Guo-quan. 2013

[10]Efficient Sugarcane Transformation via bar Gene Selection. Wang, W. Z.,Yang, B. P.,Feng, C. L.,Wang, J. G.,Xiong, G. R.,Zhao, T. T.,Zhang, S. Z.. 2017

[11]NAC transcription factors in plant multiple abiotic stress responses: progress and prospects. Shao, Hongbo,Shao, Hongbo,Shao, Hongbo,Wang, Hongyan,Tang, Xiaoli,Wang, Hongyan. 2015

[12]Detection of unintended effects in genetically modified herbicide-tolerant (GMHT) rice in comparison with non-target phenotypic characteristics. Xiao, Guoying,Jiang, Xianbin,Jiang, Xianbin. 2010

[13]Improved phosphorus acquisition by tobacco through transgenic expression of mitochondrial malate dehydrogenase from Penicillium oxalicum. Lu, Jun,Gao, Xiaorong,Yi, Jun,An, Lijia,Dong, Zhimin. 2012

[14]Efficacy of transgenic Bt cotton for resistance to the Asian corn borer (Lepidoptera : Crambidae). He, KL,Wang, ZY,Bai, SX,Zheng, L,Wang, YB,Cui, HY. 2006

[15]Influences of the disease resistance conferred by the individual transgenes, Pi-d2, Pi-d3 and Xa21, on the transgenic rice plants in yield and grain quality. Hao, Z. N.,Wang, L. P.,Tao, R. X.,Wang, J.,Wang, J.. 2009

[16]Recent Advances in Utilizing Transcription Factors to Improve Plant Abiotic Stress Tolerance by Transgenic Technology. Wang, Hongyan,Wang, Honglei,Shao, Hongbo,Shao, Hongbo,Tang, Xiaoli. 2016

[17]Development of monoclonal antibody-based sensitive ELISA for the determination of Cry1Ie protein in transgenic plant. Zhang, Yuwen,Wang, Guoying,Liu, Yunjun,Zhang, Yuwen,Zhang, Wei,Liu, Yan,Wang, Jianhua.

[18]Phytodegradation of organophosphorus compounds by transgenic plants expressing a bacterial organophosphorus hydrolase. Wang, Xiaoxue,Wu, Ningfeng,Guo, Jun,Chu, Xiaoyu,Tian, Jian,Fan, Yunliu,Yao, Bin.

[19]Over-expression of the Gr5(aroA) gene from glyphosate-contaminated soil confers high tolerance to glyphosate in tobacco. Wang, Jin,Lu, Wei,Zhu, Yu,Lin, Min,Zuo, Kaijing,Tang, Kexuan,Ye, Chun. 2014

[20]Comparative proteomics of Bt-transgenic and non-transgenic cotton leaves. Wang, Limin,Wang, Xuchu,Jin, Xiang,Jia, Ruizong,Huang, Qixing,Tan, Yanhua,Guo, Anping,Wang, Limin. 2015

作者其他论文 更多>>