The genomic and biological characterization of Citrullus lanatus cryptic virus infecting watermelon in China

文献类型： 外文期刊

第一作者： Liu, Wenwen

作者： Liu, Wenwen;Wang, Xifeng;Cao, Mengji;Ren, Yingdang;Lu, Chuantao

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关键词： Watermelon (Citrullus lanatus);Next generation sequencing (NGS);Citrullus lanatus cryptic virus (CiLCV);Full genome;Seed-borne

期刊名称：VIRUS RESEARCH （ 影响因子：3.303； 五年影响因子：3.445 ）

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收录情况： SCI

摘要： A dsRNA virus was detected in the watermelon (Citrullus lanatus) samples collected from Kaifeng, Henan province, China through the use of next generation sequencing of small RNAs. The complete genome of this virus is comprised of dsRNA-1 (1603 nt) and dsRNA-2 (1466 nt), both of which are single open reading frames and potentially encode a 54.2 kDa RNA-dependent RNA polymerase (RdRp) and a 45.9 kDa coat protein (CP), respectively. The RdRp and CP share the highest amino acid identities 85.3% and 75.4% with a previously reported Israeli strain Citrullus lanatus cryptic virus (CiLCV), respectively. Genome comparisons indicate that this virus is the same species with CiLCV, whereas the reported sequences of the Israeli strain of CiLCV are partial, and our newly identified sequences can represent the complete genome of CiLCV. Futhermore, phylogenetic tree analyses based on the RdRp sequences suggest that CiLCV is one member in the genus Deltapartitivirus, family Partitiviridae. In addition, field investigation and seed-borne bioassays show that CiLCV commonly occurs in many varieties and is transmitted though seeds at a very high rate. (C) 2017 Elsevier B.V. All rights reserved.

分类号： R37

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