Flower-like ZnO nanostructure assisted loop-mediated isothermal amplification assay for detection of Japanese encephalitis virus

文献类型： 外文期刊

第一作者： Ma, Yonghua

作者： Ma, Yonghua;Guan, Guiquan;Luo, Jianxun;Niu, Qingli;Liu, Junlong;Yin, Hong;Liu, Guangyuan;Ma, Yonghua;Lu, Yan

作者机构：

关键词： ZnO nanoflower;Hydrothermal;Japanese encephalitis virus (JEV);Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP)

期刊名称：VIRUS RESEARCH （ 影响因子：3.303； 五年影响因子：3.445 ）

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收录情况： SCI

摘要： In this study, we described a novel and effective flower-like ZnO nanostructure assisted Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) method to detect Japanese Encephalitis Virus (JEV). The effects of different concentrations of ZnO nanoflower on the RT-LAMP reaction were investigated. With the increase of concentration of ZnO nanoflower, RT-LAMP reaction obtained optimization, until the concentration exceeded 1.5 nM, RT-LAMP reaction was inhibited. Made 1 nM as optimum concentration of ZnO nanoflower, we found that optimum RT-LAMP reaction temperature and time were 60 degrees C and 30 min, respectively. The optimization might be connected with good adsorption to DNA and thermal conductivity of ZnO nanoflower, but mechanism of the RT-LAMP reaction affected by ZnO nanoflower needs to be explored further. (C) 2017 Elsevier B.V. All rights reserved.

分类号： R37

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