Pig BVDV-2 non-structural protein (N-pro) links to cellular antiviral response in vitro

文献类型: 外文期刊

第一作者: Tao, Jie

作者: Tao, Jie;Liao, Jinhu;Wang, Jianye;Zhang, Xinjun;Zhang, Qian;Zhu, Liqian;Zhu, Guoqiang;Tao, Jie;Liao, Jinhu;Wang, Jianye;Zhang, Xinjun;Zhang, Qian;Zhu, Liqian;Zhu, Guoqiang;Tao, Jie;Liu, Huili;Zhang, Weiping

作者机构:

关键词: Pig;BVDV-2;Infectious cDNA clone;Non-structural protein;Antiviral response

期刊名称:VIRUS GENES ( 影响因子:2.332; 五年影响因子:2.0 )

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收录情况: SCI

摘要: In this study, we constructed for the first time a full-length cDNA clone of pig-original bovine viral diarrhea virus 2 (BVDV-2) strain SH-28, modified the cDNA clone (pASH28) for mutant pASH Delta N-pro and derived virus strain vASH Delta N-pro by deleting the genomic region encoding the N-pro polypeptide, and examined significance of protein N-pro for antiviral responses in vitro. Data showed that N-pro-deletion mutant virus vASH Delta N-pro led to significant overexpression of oligo adenylate synthetase (OAS), myxovirus-resistant protein 1 (Mx1), and ubiquitin-like protein 15 (ISG15). Data also revealed that overexpression of N-pro, but not NS2 and NS3 proteins, resulted in significant down-regulation of OAS, Mx1, and ISG15 production (p <= \0.05) in bovine cells as well as porcine cells transfected with N-pro recombinant eukaryotic expression plasmids. N-pro (but not NS2 and NS3) was also found to inhibit poly(IC) from inducing production of type I interferon (IFN-I). These results indicated that protein N-pro may play multiple roles in regulating antiviral response in host cells interfered by pig BVDV-2 strain, and provided useful information to understand better the mechanism of BVDV-2 persistent infection in pigs.

分类号: R37

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