Lipopolysaccharide and heat stress impair the estradiol biosynthesis in granulosa cells via increase of HSP70 and inhibition of smad3 phosphorylation and nuclear translocation

文献类型： 外文期刊

第一作者： Li, Hui

作者： Li, Hui;Cai, Liuping;Shi, Zhendan;Guo, Shuangshuang;Ma, Weiming

作者机构：

关键词： Lipopolysaccharide;Heat stress;Granulosa cell;Estradiol;HSP70;Smad3

期刊名称：CELLULAR SIGNALLING （ 影响因子：4.315； 五年影响因子：4.206 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： LPS and heat stress have been shown to exert various toxic effects in animals, as they induce estradiol biosynthesis dysfunction in granulosa cells (GCs) and result in low reproductive performance. However, there is limited information regarding their detailed mechanisms. In the present study, primary cultured porcine GCs were treated with LPS (1000 ng/mL for 48 h), or heat stress (41 degrees C for 3 h), in vitro, with or without the HSP70 inhibitor VER155008 (10 mu M), to investigate their potential mechanisms. To mimic the spike in HSP70 from LPS and heat stress, treatments with only the HSP70 activator STA-4783 (10 mu M for 3 h or 48 h) were also performed. We found that LPS and heat stress treatments could significantly reduce the expressions of FSHR and CYP19A1; associated with a reduction in estradiol concentrations; and increased in HSP70 expression both at mRNA and protein levels. While, VER155008 attenuation of LPS and heat stress induced HSP70 upregulation can restore the expressions of FSHR and CYP19A1. Furthermore, STA-4783 treatment alone can mimic the effects of LPS and heat stress treatments. Following immunofluorescence staining and western blot analysis showed that Smad3 phosphorylation and nuclear translocation were also inhibited by LPS, heat stress and STA-4783 treatments. We also examined the interactions between HSP70 and Smad3 by yeast two-hybrid screening, the results revealed that HSP70 indirectly interacted with Smad3. Thus, our results suggested that LPS and heat stress could impair estradiol biosynthesis in GCs via increased HSP70 and indirect inhibition of Smad3 phosphorylation and nuclear translocation. (C) 2016 Elsevier Inc. All rights reserved.

分类号： Q24

  • 相关文献

[1]Association analysis of HSP70A1A haplotypes with heat tolerance in Chinese Holstein cattle. Xiong, Qi,Liu, Yang,Suo, Xiaojun,Zhang, Nian,Li, Xiaofeng,Chen, Mingxin,Xiong, Haiqian,Li, Wengong,Huang, Tao,Chai, Jin,Jiang, Siwen,Chai, Jin,Jiang, Siwen.

[2]Effects of N-carbamylglutamate and L-arginine on steroidogenesis and gene expression in bovine granulosa cells. Feng, T.,Schutz, L. F.,Morrell, B. C.,Perego, M. C.,Spicer, L. J.. 2018

[3]Systemic analysis of gene expression profiles in porcine granulosa cells during aging. Li Hui,Guo Shuangshuang,Yu Jianning,Shi Zhendan. 2017

[4]Expression, Regulation, and Functional Characterization of FST Gene in Porcine Granulosa Cells. Zhou, QuanYong,Wan, MingChun,Wei, QiPeng,Song, QiongLi,Xiong, LiGen,Huo, JunHong,Huang, JiangNan.

[5]Efficiency of in vitro embryo production of yak (Bos grunniens) cultured in different maturation and culture conditions. Guo, Xian,Ding, Xuezhi,Pei, Jie,Bao, Pengjia,Liang, Chunnian,Chu, Min,Yan, Ping,Guo, Xian,Ding, Xuezhi,Pei, Jie,Bao, Pengjia,Liang, Chunnian,Chu, Min,Yan, Ping. 2012

[6]Transcriptome sequencing analysis of porcine granulosa cells treated with an anti-inhibin antibody. Lei, Mingming,Cai, Liuping,Li, Hui,Chen, Zhen,Shi, Zhendan. 2017

[7]Fas-Associated Death Domain (FADD) Mediated Activation of a Apoptosis Program in Bovine Follicular Granulosa Cells. Yang, Run-Jun,Li, Jun-Ya,Gao, Xue,Zhang, Lu-Pei,Gao, Hui-Jiang,Xu, Shang-Zhong,Yang, Run-Jun,Zhao, Zhi-Hui. 2011

[8]Regulation of FoxO1 transcription factor by nitric oxide and cyclic GMP in cultured rat granulosa cells (Retracted article. See vol 24, pg 281, 2007). Li, Xuebin,Jiang, Yongqing,Wang, Zhengchao,Liu, Gentao,Hutz, Reinhold J.,Liu, Wenbin,Xie, Zhuang,Shi, Fangxiong. 2005

[9]NPR1-dependent salicylic acid signaling is not involved in elevated CO2-induced heat stress tolerance in Arabidopsis thaliana. Li, Xin,Ahammed, Golam Jalal,Li, Xin,Yu, Jingquan,Shi, Kai. 2015

[10]Dietary -arginine supplementation enhances placental growth and reproductive performance in sows. Gao, Kaiguo,Jiang, Zongyong,Lin, Yingcai,Zheng, Chuntian,Zhou, Guilian,Chen, Fang,Gao, Kaiguo,Yang, Lin,Wu, Guoyao. 2012

[11]Comparative responses in rare minnow exposed to 17 beta-estradiol during different life stages. Liao, T.,Guo, Q. L.,Xu, Y.,Liao, T.,Cheng, W.,Jin, S. W.. 2009

[12]Bidirectional Estrogen-Like Effects of Genistein on Murine Experimental Autoimmune Ovarian Disease. Ding, Qiao,Wang, Yuxiao,Li, Na,Hu, Jielun,Nie, Shaoping,Zhu, Kexue,Wang, Sunan,Zhu, Fan. 2016

[13]Effect of SIRT1 on cellular apoptosis and steroidogenesis in bovine ovarian granulosa cells in vitro. Tan, X. W.,You, W.,Song, E. L.,Zhao, H. B.,Liu, X. M.,Wang, H. Z.,Liu, G. F.,Cheng, H. J.,Liu, Y. F.,Wan, F. C.,Tan, X. W.,You, W.,Song, E. L.,Zhao, H. B.,Liu, X. M.,Wang, H. Z.,Liu, G. F.,Cheng, H. J.,Liu, Y. F.,Wan, F. C..

[14]Establishment of a lateral flow colloidal gold immunoassay strip for the rapid detection of estradiol in milk samples. Yang, Xing-dong,Wang, Fang-yu,Zhang, Gai-ping,Yang, Xing-dong,Zeng, Xian-yin,Song, Chun-mei,Wu, Shu-yan,Zhang, Gai-ping.

[15]miR-15b negatively correlates with lipid metabolism in mammary epithelial cells. Chu, Meiqiang,Zhao, Yong,Yu, Shuai,Hao, Yanan,Zhang, Pengfei,Feng, Yanni,Ma, Dongxue,Li, Lan,Shen, Wei,Min, Lingjiang,Zhang, Hongfu,Liu, Jing,Cheng, Ming,Cao, Hongfang,Li, Qiang. 2018

[16]MicroRNA-126 participates in lipid metabolism in mammary epithelial cells. Chu, Meiqiang,Zhao, Yong,Feng, Yanni,Li, Lan,Shen, Wei,Min, Lingjiang,Zhang, Hongfu,Liu, Jing,Cheng, Ming,Cao, Hongfang,Li, Qiang. 2017

[17]Mammalian steroid hormones can reduce abundance and affect the sex ratio in a soil nematode community. Hu, Cheng,Hermann, Galit,Pen-Mouratov, Stanislav,Steinberger, Yosef,Hu, Cheng,Shore, Laurence. 2011

[18]Butyric acid regulates progesterone and estradiol secretion via cAMP signaling pathway in porcine granulosa cells. Lu, Naisheng,Li, Mengjiao,Lei, Hulong,Jiang, Xueyuan,Tu, Weilong,Lu, Yang,Xia, Dong.

[19]Accumulation and subcellular localization of heat shock proteins in young grape leaves during cross-adaptation to temperature stresses. Zhang, Jun-Huan,Pan, Qiu-Hong,Zhan, Ji-Cheng,Huang, Wei-Dong,Zhang, Jun-Huan,Wang, Yu-Zhu,Wang, Li-Jun. 2008

[20]Effect of High Dietary Carbohydrate on Growth, Serum Physiological Response, and Hepatic Heat Shock Protein 70 Expression of Wuchang Bream (Megalobrama amblycephala) at Two Temperatures. Zhou, Chuanpeng,Liu, Bo,Xie, Jun,Ge, Xianping,Liu, Bo,Xie, Jun,Ge, Xianping. 2013

作者其他论文 更多>>

微信小程序