An in vitro larval migration assay for assessing anthelmintic activity of different drug classes against Ascaris suum

文献类型： 外文期刊

第一作者： Zhao, Jianguo

作者： Zhao, Jianguo;Song, Shuaibao;Han, Qian;Zhao, Jianguo;Song, Shuaibao;Han, Qian;Zhao, Jianguo;Han, Qian;Zhao, Jianguo;Cai, Jianping;Han, Qian;Williams, Andrew R.;Hansen, Tina Vicky Alstrup;Thamsborg, Stig M.;Chen, Gang;Kang, Ming;Zhang, Zhuangzhi;Liu, Qun

作者机构：

关键词： Parasitic nematode;Ascaris suum;Anthelmintic;Larval migration assay;Benzimidazole;Agar

期刊名称：VETERINARY PARASITOLOGY （ 影响因子：2.738； 五年影响因子：2.951 ）

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收录情况： SCI

摘要： In vitro methods have been developed for the detection of anthelmintic resistance in a range of nematode species. However, the life cycle of Ascaris suum renders the commonly used egg hatch assay and larval development assay unusable. In this study we developed a combined multi-well culture and agar gel larval migration assay to test the effect of benzimidazole and tetrahydropyrimidinfimidazothiazole anthelmintics against nine isolates of A. suum collected from locations in China and Denmark. Drugs tested were thiabendazole, fenbendazole, mebendazole, levamisole, and pyrantel. The percentages of larvae that migrated to the surface of each treated and control well were used to calculate the drug concentration which inhibits 50% of the larvae migration (EC50). The values of EC50 of thiabendazole, fenbendazole, mebendazole, levamisole, and pyrantel against A. suum isolates ranged 74-150, 4.9-13.9, 2.3-4.3, 358-1150 and 1100-4000 nM, respectively. This combined multi-well culture and agar gel larval migration assay was a sensitive bioassay for anthelmintic activity and could serve as an in vitro method to detect for lowered drug efficacy against A. suum or possibly to screen for anthelmintic drug candidates. (C) 2017 Elsevier B.V. All rights reserved.

分类号： S85

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