Molecular Characterization of Chinese Grape Landraces (Vitis L.) Using Microsatellite DNA Markers
文献类型: 外文期刊
第一作者: Li, Beibei
作者: Li, Beibei;Jiang, Jianfu;Fan, Xiucai;Zhang, Ying;Sun, Haisheng;Liu, Chonghuai;Li, Beibei;Zhang, Guohai
作者机构:
关键词: grapevine cultivars;SSR;microsatellite characterization;cultivar identification;Vitis
期刊名称:HORTSCIENCE ( 影响因子:1.455; 五年影响因子:1.617 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: In this study, we present the molecular characterization of 61 Chinese grape landraces and 33 foreign cultivars by using nine microsatellite DNA markers. A total of 115 distinct alleles were amplified, and the average allele number was 12.78. The average observed and expected heterozygosity values were 0.797 and 0.839, respectively. The effective allele numbers ranged from 5.011 to 8.575. The average polymorphism information content (PIC) was 0.816. Eighty distinct genotypes were detected, and new synonyms and homonyms were found. The clustering dendrogram indicated that 94 Vitis materials could be divided into five major groups, and the cluster analysis showed that part of the Chinese grape landraces had a close relationship with the foreign cultivars. Assessment of the true cultivar identity, and the identification of synonyms and homonyms will be a contribution to improve the grape germplasm management and protect breeders' intellectual rights.
分类号: S6
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