Prophenoloxidase encoding genes in Spodoptera exigua: molecular cloning and immune response to the challenge of nucleopolyhedrovirus and/or Microplitis pallidipes

文献类型： 外文期刊

第一作者： Zhang, Hao

作者： Zhang, Hao;Ji, Xiang-Yun;Wan, Nian-Feng;Jiang, Jie-Xian

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关键词： Insect immunity;Microplitis pallidipes;nucleopolyhedrovirus;prophenoloxidase;Spodoptera exigua

期刊名称：BIOCONTROL SCIENCE AND TECHNOLOGY （ 影响因子：1.665； 五年影响因子：1.616 ）

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收录情况： SCI

摘要： Phenoloxidase (PO) catalyses the synthesis of o-quinones, which plays critical roles in the innate immunity of insects. The un-activated form of PO is prophenoloxidase (PPO), which has been widely reported, especially in connection with the immune response of insects to invading organisms or chemicals. However, connection between PPO and challenge by combinations of two biocontrol agents (parasitoids and virus) is not well known. We cloned the cDNA encoding an insect PPO (designated as SePPO1) from the beet armyworm, Spodoptera exigua (Hubner), and studied the response of SePPO to the challenge of Microplitis pallidipes Szepligeti and Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV). The results indicated that SePPO1 had an open reading frame of 2055 bp. Sequence alignment and phylogenetic analysis showed that SePPO1 shared 51% identity with SePPO2, and the two SePPO genes were positively correlated in mRNA levels, which indicated that they act synergistically. The two SePPOs were mainly expressed in older instar larvae, especially in haemolymph. Parasitism by M. pallidipes inhibited PPO transcription and PO activity, and SeMNPV infection induced the upregulation of PPO transcription and increased PO activity 72 h after treatment. And in the combined treatment, PO activity and PPO transcription were downregulated, which indicates that parasitism suppressed the upregulation induced by SeMNPV infection.

分类号： S476

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