Validation of a set of informative simple sequence repeats markers for variety identification in Pak-choi (Brassica rapa L. ssp chinensis var. communis)

文献类型： 外文期刊

第一作者： Su, Tongbing

作者： Su, Tongbing;Zhao, Xiuyun;Sui, Guanglei;Yu, Shuancang;Zhang, Fenglan;Wang, Weihong;Zhang, Deshuang;Yu, Yangjun;Su, Tongbing;Zhao, Xiuyun;Sui, Guanglei;Yu, Shuancang;Zhang, Fenglan;Wang, Weihong;Zhang, Deshuang;Yu, Yangjun;Su, Tongbing;Zhao, Xiuyun;Sui, Guanglei;Yu, Shuancang;Zhang, Fenglan;Wang, Weihong;Zhang, Deshuang;Yu, Yangjun

作者机构：

关键词： Pak-choi (Brassicarapa ssp chinensis var. communis);simple sequence repeat;DNA fingerprinting;variety identification

期刊名称：PLANT BREEDING （ 影响因子：1.832； 五年影响因子：1.956 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A core set of 21 simple sequence repeats (SSR) markers was developed for Pak-choi (Brassicarapa ssp.chinensis var.communis) variety identification. We initially selected 74 SSR markers which exhibited high polymorphism and reproducibility in SSR detection from 2129 SSRs. Using the 74 SSR-based dendrogram for 45 inbred lines as calibration, 21 core SSRs were selected out. The utility of this core set SSRs was firstly tested in 45 inbred lines and finally verified in 102 commercial varieties. We also constructed a molecular ladder for each core SSR as a reference standard. Diversity analysis of this core SSR panel in 102 varieties demonstrated that each marker generates 2-3 alleles (averaged 2.33), with polymorphism information content values ranging from 0.01 to 0.56 (averaged 0.31). The averaged values of Shannon information index, observed heterozygosity, expected heterozygosity and Wright's fixation index were 0.59, 0.43, 0.38 and -0.09, respectively. Furthermore, the 21 SSR-based classifications for 102 varieties were consistent with traditional classification based on morphology. This core SSR panel represents an effective tool for genetic variation analysis in Pak-choi.

分类号： S3

  • 相关文献

[1]Development of maizeSNP3072, a high-throughput compatible SNP array, for DNA fingerprinting identification of Chinese maize varieties. Tian, Hong-Li,Wang, Feng-Ge,Zhao, Jiu-Ran,Yi, Hong-Mei,Wang, Lu,Wang, Rui,Yang, Yang,Song, Wei.

[2]MELON SEED VARIETY IDENTIFICATION BASED ON HYPERSPECTRAL TECHNOLOGY COMBINED WITH DISCRIMINANT ANALYSIS. Li, Cuiling,Fan, Pengfei,Jiang, Kai,Wang, Xiu,Feng, Qingchun,Zhang, Chunfeng,Li, Cuiling,Fan, Pengfei,Jiang, Kai,Wang, Xiu,Feng, Qingchun,Zhang, Chunfeng. 2017

[3]Studies on the DNA Barcoding of Two Newly Discovered Chicken Breeds by mtDNA COI Gene. Gao Yu-Shi,Tu Yun-Jie,Tang Xiu-Jun,Lu Jun-Xian,Zhang Xiao-Yan. 2011

[4]Study on Varieties Identification of Kentucky Bluegrass Using Hyperspectral Imaging and Discriminant Analysis. Xiao Bo,Liang Xiao-hong,Zhang Li-juan,Han Lie-bao,Xiao Bo,Han Lie-bao,Mao Wen-hua. 2012

[5]Spectral identification of melon seeds variety based on k-nearest neighbor and Fisher discriminant analysis. Li, Cuiling,Jiang, Kai,Zhao, Xueguan,Fan, Pengfei,Wang, Xiu,Liu, Chuan,Li, Cuiling,Jiang, Kai,Zhao, Xueguan,Fan, Pengfei,Wang, Xiu,Liu, Chuan. 2017

[6]Melon Seeds Variety Identification Based on Chlorophyll Fluorescence Spectrum and Reflectance Spectrum. Li Cui-ling,Jiang Kai,Feng Qing-chu,Wang Xin,Meng Zhi-jun,Wang Song-lin,Gao Yuan-yuan,Li Cui-ling,Jiang Kai,Feng Qing-chu,Wang Xin,Wang Song-lin,Gao Yuan-yuan. 2018

[7]AFLP fingerprinting of Chinese epidemic strains of Puccinia striiformis f. sp tritici. Liu, F,Kang, ZS,Chen, SY,Li, ZQ,Wu, LR. 2001

[8]ESTABLISHMENT OF DNA FINGERPRINTING IN CLONAL TEA IMPROVED CULTIVARS FROM YUNNAN OF CHINA USING ISSR MARKERS. Liu, B. Y.,Zhao, C. M.,Sun, X. M.,Li, Y. Y.,Jiang, H. B.,Wang, Y. G.,Ma, L.,Duan, Z. F.. 2015

[9]Development and characterization of a core set of SSR markers for fingerprinting analysis of Chinese maize varieties. Wang, Feng-Ge,Tian, Hong-Li,Zhao, Jiu-Ran,Yi, Hong-Mei,Wang, Lu,Song, Wei. 2011

[10]Studies on fish heterosis with DNA fingerprinting. Wang, J,Xia, D.

[11]Analyzing genetic relationships in Bombyx mori using intersimple sequence repeat amplification. Li, Muwang,Hou, Chengxiang,Miao, Xuexia,Xu, Anying,Huang, Yongping.

[12]Molecular Marker Systems for Profiling and Diversity Analysis of Rice Germplasm in Philippine Genebank. Romero, Gabriel O.,Yong, Wan. 2009

[13]Genetic diversity in Puccinia striiformis Westend. f.sp. tritici revealed by pathogen genome-specific repetitive sequence. Chen, SY,Kang, ZS,Wu, LR,Li, ZQ. 1998

[14]Development of a maize 55 K SNP array with improved genome coverage for molecular breeding. Xu, Cheng,Jian, Yinqiao,Guo, Zifeng,Xie, Chuanxiao,Fu, Junjie,Wang, Hongwu,Wang, Guoying,Xu, Yunbi,Zou, Cheng,Ren, Yonghong,Zhang, Yan,Xu, Yunbi,Li, Ping,Li, Ping. 2017

[15]Construction of a high-density linkage map and mapping quantitative trait loci for somatic embryogenesis using leaf petioles as explants in upland cotton (Gossypium hirsutum L.). Zhenzhen Xu,Chaojun Zhang,Xiaoyang Ge,Ni Wang,Kehai Zhou,Xiaojie Yang,Zhixia Wu,Xueyan Zhang,Chuanliang Liu,Zuoren Yang,Changfeng Li,Kun Liu,Zhaoen Yang,Yuyuan Qian,Fuguang Li.

[16]Constructing a high-density linkage map for Gossypium hirsutum x Gossypium barbadense and identifying QTLs for lint percentage. Yuzhen Shi,Wentan Li,Aiguo Li,Ruihua Ge,Baocai Zhang,Junzhi Li,Guangping Liu,Junwen Li,Aiying Liu,Haihong Shang,Juwu Gong,Wankui Gong,Zemao Yang,Feiyü Tang,Zhi Liu,Weiping Zhu,Jianxiong Jiang,Xiaonan Yu,Tao Wang,Wei Wang,Tingting Chen,Kunbo Wang,Zhengsheng Zhang,Youlu Yuan. 2015

[17]Microsatellite Enrichment by Magnetic Beads in Chrysanthemum. Li, Y. H.,Luo, C.,Wu, Z. Y.,Zhang, X. H.,Cheng, X.,Huang, C. L.,Li, Y. H.,Dong, R.. 2013

[18]Development and use of EST-SSR markers for assessing genetic diversity in the brown planthopper (Nilaparvata lugens Stal). Jing, S.,Liu, B.,Peng, L.,Peng, X.,Zhu, L.,He, G.,Fu, Q.. 2012

[19]Molecular characterization of Fusarium head blight resistance from wheat variety Wangshuibai. Zhang, X,Zhou, MP,Ren, LJ,Bai, GH,Ma, HX,Scholten, OE,Guo, PG,Lu, WZ. 2004

[20]Mapping of a wheat resistance gene to yellow mosaic disease by amplified fragment length polymorphism and simple sequence repeat markers. Nie, H,He, ZT,Chen, XL,Han, YP,Wang, JR,Li, X,Han, CG,Yu, JL. 2005

作者其他论文 更多>>

微信小程序